DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Abstract
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or...
Paper Details
Title
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Published Date
Nov 1, 2015
Journal
Volume
33
Issue
11
Pages
1162 - 1164
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