Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.

Volume: 67, Issue: 1, Pages: 6 - 20
Published: Jan 1, 2009
Abstract
Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in microbial ecology to quantify the abundance and expression of taxonomic and functional gene markers within the environment. Q-PCR-based analyses combine 'traditional' end-point detection PCR with fluorescent detection technologies to record the accumulation of amplicons in 'real time' during each cycle of the PCR amplification. By detection of amplicons during the...
Paper Details
Title
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.
Published Date
Jan 1, 2009
Volume
67
Issue
1
Pages
6 - 20
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