Characterization of the bilin attachment sites in R-phycoerythrin.

The amino acid sequence around the sites of attachment of all the bilin prosthetic groups of Gastroclonium coulteri R-phycoerythrin, (&)gy, have been determined.The sequences of tryptic peptides derived from the a and fl subunits are a-1 PEB Cys*-Tyr-Arg a-2 PEB Leu-Cys*-Val-Pro-Arg 8-1 PEB Met-Ala-Ala-Cys*-Leu-Arg 8-2 PEB Ile-Asp-Ile-Ala-Ala-Gly-Asp-Cys*-(Ser)-Ala-Leu-Ser-Ser-Glu-Val-Ala-Ser-Tyr-Cys-Asp-Arg 8-3 PUB...