Match!

Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing.

Published on Dec 1, 1994in Journal of Clinical Microbiology4.959
Timothy Barrett53
Estimated H-index: 53
,
H. Lior2
Estimated H-index: 2
+ 6 AuthorsPatricia M. Griffin70
Estimated H-index: 70
Abstract
Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the outbreak had the same phage type as the outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing outbreak and non-outbreak-related strains. The PFGE patterns of seven preoutbreak sporadic isolates and five sporadic isolates from the outbreak period differed from that of the outbreak strain by a single band, making it difficult to identify these isolates as outbreak or non-outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates. Images
  • References (0)
  • Citations (321)
📖 Papers frequently viewed together
1,549 Citations
7,145 Citations
816 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References0
Newest
Cited By321
Newest
Background Urinary tract infections caused by extended-spectrum beta-lactamase-producing bacteria are increasing worldwide. At our hospital, the number of pediatric patients hospitalized because of an upper urinary tract infection has dramatically increased since 2016. In total, 60.5% of urinary tract infections are caused by extended-spectrum beta-lactamase-producing Escherichia coli. Such a high prevalence of extended-spectrum beta-lactamase-producing E. coli has not been detected previously i...
Source
Last. Burton W. BlaisH-Index: 16
view all 4 authors...
1 CitationsSource
#1H. StrydomH-Index: 1
#2Jing WangH-Index: 8
Last. J. WrightH-Index: 1
view all 6 authors...
: Incidence of human yersiniosis in New Zealand has increased between 2013 and 2017. For surveillance and outbreak investigations it is essential that an appropriate level of discrimination between pathogenic Yersinia enterocolitica isolates is provided, in order to support epidemiological linking of connected cases. Subtyping of 227 Y. enterocolitica isolates was performed using a range of different typing methods, including biotyping, serotyping and seven loci multiple-locus variable-number ta...
4 CitationsSource
#1Margaret D. Weinroth (CSU: Colorado State University)H-Index: 5
#2Brianna C. Britton (CSU: Colorado State University)H-Index: 1
Last. Keith E. Belk (CSU: Colorado State University)H-Index: 46
view all 3 authors...
Abstract Detection, reduction, and public health monitoring of foodborne pathogens has advanced in precision and efficiency as technology has progressed. Here, we look back on the evolution of food safety management and public health, and attempt to provide a view of how the technology and tools have changed, and how emerging technologies and tools may impact how we manage food safety and public health in the future. With the revolution of gene editing techniques (e.g. CRISPR-Cas9, etc.), Next G...
1 CitationsSource
#1Rafaela Fernandes Zancan (USP: University of São Paulo)H-Index: 4
#2Lyz Cristina Furquim Canali (USP: University of São Paulo)H-Index: 2
Last. Marco Antonio Hungaro Duarte (USP: University of São Paulo)H-Index: 34
view all 6 authors...
: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the f...
2 CitationsSource
#1Jun TerajimaH-Index: 28
#2Hidemasa Izumiya (NIH: National Institutes of Health)H-Index: 25
Last. Makoto Ohnishi (NIH: National Institutes of Health)H-Index: 41
view all 4 authors...
: Shiga toxin (verotoxin)-producing Escherichia coli (STEC) is an important cause of foodborne disease. Since outcomes of the infections with STEC have a broad range of manifestation from asymptomatic infection or mild intestinal discomfort, to bloody diarrhea, hemolytic uremic syndrome (HUS), end-stage renal disease (ESRD), and death, the disease is a serious burden in public health and classified as a notifiable infectious disease in many countries. Cattle and other ruminants are considered to...
3 CitationsSource
#1Sinem Acar (METU: Middle East Technical University)H-Index: 4
#2Yeşim Soyer (METU: Middle East Technical University)H-Index: 12
Due to health benefits of the fresh fruits and vegetables, the consumption of the minimally processed refrigerated (MPR) and packaged produce has been increased in the USA and EU countries. For MPR and packaged product production, fresh produces and vegetables are first examined for quality purposes and then washed, cut, dried, sanitized, and packaged. When these processes are performed under insufficient sanitary conditions, foodborne outbreaks become inevitable. The most common foodborne agent...
Source
: In 2013, two outbreaks of enterohemorrhagic Escherichia coli (EHEC) occurred in Saitama city. According to reports from each of the medical institutions that detected the EHEC isolates, the isolates seemed to differ in their production of Vero Toxin (VT / Shiga Toxin: Stx) since one isolate produced only Stx1 and the other produced both Stx1 and Stx2. However, a patient survey conducted by a public health center revealed that common foodstuffs had been consumed in both outbreaks. Because, the ...
Source
#1Jennifer Ronholm (Health Canada)H-Index: 10
#1Jennifer Ronholm (Health Canada)H-Index: 4
Last. Franco Pagotto (Health Canada)H-Index: 21
view all 4 authors...
SUMMARY The epidemiological investigation of a foodborne outbreak, including identification of related cases, source attribution, and development of intervention strategies, relies heavily on the ability to subtype the etiological agent at a high enough resolution to differentiate related from nonrelated cases. Historically, several different molecular subtyping methods have been used for this purpose; however, emerging techniques, such as single nucleotide polymorphism (SNP)-based techniques, t...
63 CitationsSource