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Antithrombotic effect of L-arginine in hypertensive rats.

Published on Jan 1, 2004in Journal of Physiology and Pharmacology 2.54
Dorota Cylwik3
Estimated H-index: 3
,
Andrzej Mogielnicki14
Estimated H-index: 14
+ 2 AuthorsBuczko W11
Estimated H-index: 11
Abstract
The aim of the study was to evaluate the effect of L-arginine (L-Arg) on haemostasis in stasis model of venous thrombosis in renal hypertensive rats. The effect of the single dose (i.v.300 mg/kg bolus+300 mg/kg/h) and of the 10-day application (p.o. 1 g/kg, once daily) of L-Arg was determined. L-Arg reduced the blood pressure both in the acute and long-term application. The single dose of L-Arg decreased the occurrence rate of the thrombus whereas long-term administration reduced significantly the thrombus weight. There were no differences in prothrombin time and activated partial thromboplastin time while the fibrinogen concentration decreased both in the acute and the long-term experiment. L-Arg shortened euglobulin clot lysis time and bleeding time in the long-term application. The chronic L-Arg treatment also inhibited significantly collagen-induced platelet aggregation. The overall haemostasis and coagulation potentials were inhibited and the fibrinolysis potential was higher in the group receiving this amino-acid. The results show that L-Arg, in a complex way, evokes the antithrombotic effect in the model of venous thrombosis in hypertensive rats.
  • References (31)
  • Citations (14)
References31
Newest
Published on Jan 28, 2003in Circulation 23.05
PierMarco Piatti23
Estimated H-index: 23
,
Gabriele Fragasso16
Estimated H-index: 16
+ 8 AuthorsSergio Luigi Chierchia2
Estimated H-index: 2
Background— We tested the hypothesis that asymmetric dimethylarginine (ADMA) levels could be elevated and influence endothelin-1 and nitric oxide release and action in patients with cardiac syndrome X (CSX). In addition, we evaluated whether an intravenous infusion of l-arginine would improve endothelial function in these subjects. Methods and Results— Nine patients with CSX and 14 control subjects underwent a continuous infusion of l-arginine (0.125 g/min) or saline for 120 minutes. Sixty minut...
Published on Jan 1, 2003in Arteriosclerosis, Thrombosis, and Vascular Biology 6.62
Jiqiu Chen7
Estimated H-index: 7
(Harvard University),
Peter Kuhlencordt21
Estimated H-index: 21
(Harvard University)
+ 3 AuthorsPaul L. Huang81
Estimated H-index: 81
(Harvard University)
Objective— l-Arginine serves as a substrate for the formation of NO by the NO synthase (NOS) enzymes. In some studies, dietary supplementation of l-arginine reduces atherosclerosis through the restoration of NO release and improvement in endothelial function. In the present study, we investigate the effect of l-arginine supplementation on the development of atherosclerosis in a mouse model. Methods and Results— Apolipoprotein E (apoE) knockout (ko) and apoE/inducible NOS (iNOS) double-ko mice we...
Published on May 1, 2002in Atherosclerosis 4.25
Robert B. Preli2
Estimated H-index: 2
(Wake Forest University),
Karen Potvin Klein11
Estimated H-index: 11
(Wake Forest University),
David M. Herrington71
Estimated H-index: 71
(Wake Forest University)
Abstract The vascular endothelium is acknowledged to play an important role in vascular physiology. Attention has focused on endothelial production of nitric oxide as a key element in many of the processes associated with the development of atherosclerosis. l -arginine is the substrate for the enzyme nitric oxide synthase (NOS), which is responsible for the endothelial production of nitric oxide. Therefore, many investigators have been interested in whether dietary l -arginine supplementation ca...
Published on Nov 15, 2001in Thrombosis Research 3.27
Thomas W. Stief9
Estimated H-index: 9
,
Monika Weippert1
Estimated H-index: 1
+ 1 AuthorsHarald Renz4
Estimated H-index: 4
Abstract Background: The diagnosis and the therapy of in vivo hemostasis activation is of great clinical importance. Artefactual changes of the hemostasis (i.e., coagulation or fibrinolysis) in vitro have to be prevented. Usual in vitro anticoagulation by sodium citrate does not fully inhibit coagulation—or fibrinolysis—activation. Therefore, there is need for a simple physiologic inhibitor of hemostasis activation both in diagnosis and therapy of hemostasis activation. Methods: Whole blood clot...
Published on Sep 1, 2001in Thrombosis Research 3.27
Shu He18
Estimated H-index: 18
(KI: Karolinska Institutet),
Aleksandra Antovic15
Estimated H-index: 15
(KI: Karolinska Institutet),
Margareta Blombäck52
Estimated H-index: 52
(KI: Karolinska Institutet)
To offer a suitable method for use in routine laboratories and research work, some modifications were made in the assay of overall haemostatsis potential (OHP) we developed earlier. Thrombin in a decreased dose with or without tissue-type plasminogen activator was added to plasma for initiation of fibrinogen clotting. Areas under two fibrin-aggregation curves i.e., abovementioned OHP and overall coagulation potential (OCP) were thus created. A difference between the two parameters reflects the o...
Published on May 1, 2001in Diabetes Care 15.27
PierMarco Piatti23
Estimated H-index: 23
(UniSR: Vita-Salute San Raffaele University),
Lucilla D. Monti26
Estimated H-index: 26
(UniSR: Vita-Salute San Raffaele University)
+ 6 AuthorsK. George M.M. Alberti33
Estimated H-index: 33
(Newcastle University)
OBJECTIVE —The aim of this study was to evaluate whether long-term administration of l-arginine acting through a normalization of NO/cyclic-guanosine-3′,5′-cyclic monophosphate (cGMP) pathway was able to ameliorate peripheral and hepatic insulin sensitivity in 12 lean type 2 diabetic patients. RESEARCH DESIGN AND METHODS —A double-blind study was performed for 3 months. In the first month, patients were treated with their usual diet. Then they were randomly allocated into two groups. In group 1,...
Published on Jan 1, 2001in Polish Journal of Pharmacology
Gruszecki M1
Estimated H-index: 1
,
Rółkowski R5
Estimated H-index: 5
+ 1 AuthorsWlodzimierz Buczko22
Estimated H-index: 22
To clarify if one of the most common antihypertensive drugs, propranolol, can prevent venous thrombotic process, rats were treated with propranolol (PRO; 5 mg/kg ip) in an acute or chronic (14 days) manner. Both regimens resulted in a marked reduction of the systolic blood pressure (p < 0.001) and, probably as a consequence, in the shortening of the bleeding time (p < 0.01). After ligation of the vena cava, the incidence of the venous thrombosis and the thrombus weight decreased significantly in...
Published on May 9, 2000in Circulation 23.05
Arnon Blum22
Estimated H-index: 22
,
Londa Hathaway9
Estimated H-index: 9
+ 6 AuthorsRichard O. Cannon17
Estimated H-index: 17
Background—Vascular nitric oxide (NO) bioavailability is reduced in patients with coronary artery disease (CAD). We investigated whether oral l-arginine, the substrate for NO synthesis, improves homeostatic functions of the vascular endothelium in patients maintained on appropriate medical therapy and thus might be useful as adjunctive therapy. Methods and Results—Thirty CAD patients (29 men; age, 67±8 years) on appropriate medical management were randomly assigned to l-arginine (9 g) or placebo...
Published on May 9, 2000in Circulation 23.05
Joseph Loscalzo106
Estimated H-index: 106
(BU: Boston University)
In the latter half of the nineteenth century, amyl nitrate, sodium nitrite, and nitroglycerin were each shown to relieve angina pectoris. These early observations, coupled with the rapid growth of the chemical and pharmaceutical industries, led to the development of nitrovasodilator drugs as the cornerstone of therapy for ischemic heart disease throughout most of the next century. Despite a rather thorough understanding of the pharmacology of these agents, only recently have the vascular actions...
Published on Oct 1, 1999in Thrombosis Research 3.27
Shu He18
Estimated H-index: 18
,
Katarina Bremme36
Estimated H-index: 36
(KI: Karolinska Institutet),
Margareta Blombäck52
Estimated H-index: 52
Abstract The aim of this study was to design a simple laboratory method that can screen the overall haemostatic potential in plasma (OHPP) when a hyper- or hypocoagulable state is present. A fibrin time curve was made via spectrophotometric registration of fibrin generation and lysis in plasma, to which exogenous thrombin and tissue type plasminogen activator was added. The area under the curve, calculated by the sum of absorbance (ABS-sum), varied in correlation to the concentrations of platele...
Cited By14
Newest
Published on Aug 2, 2016in Expert Opinion on Drug Metabolism & Toxicology 3.49
Emilia Sokolowska4
Estimated H-index: 4
(UMB: Medical University of Białystok),
Bartlomiej Kalaska6
Estimated H-index: 6
(UMB: Medical University of Białystok)
+ 1 AuthorsAndrzej Mogielnicki14
Estimated H-index: 14
(UMB: Medical University of Białystok)
ABSTRACTIntroduction: Unfractionated heparin is a strongly anionic anticoagulant used extensively in medicine to prevent blood clotting. In the case of an emergency bleeding in response to heparin, the protamine sulfate is administered. Despite its extensive clinical use, protamine may produce life-threatening side effects such as systemic hypotension, catastrophic pulmonary vasoconstriction or allergic reactions. Recent studies have demonstrated new organ-specific complications of the heparin r...
Published on Nov 1, 2015in Carcinogenesis 4.00
Simonetta Astigiano21
Estimated H-index: 21
,
Monica Morini26
Estimated H-index: 26
(IIT: Istituto Italiano di Tecnologia)
+ 6 AuthorsOttavia Barbieri25
Estimated H-index: 25
Arginase (ARG) is a metabolic enzyme present in two isoforms that hydrolyze l-arginine to urea and ornithine. In humans, ARG isoform 1 is also expressed in cells of the myeloid lineage. ARG activity promotes tumour growth and inhibits T lymphocyte activation. However, the two ARG transgenic mouse lines produced so far failed to show such effects. We have generated, in two different genetic backgrounds, transgenic mice constitutively expressing ARG1 under the control of the CD68 promoter in macro...
Published on Mar 1, 2015in Biotechnology Letters 2.15
Oveis Jamialahmadi2
Estimated H-index: 2
(TMU: Tarbiat Modares University),
Ahmad Fazeli6
Estimated H-index: 6
+ 1 AuthorsMohammad Reza Fazeli18
Estimated H-index: 18
(Tehran University of Medical Sciences)
Recombinant plasminogen activator (r-PA, reteplase) is an engineered variant of alteplase. When expressed in E. coli, it appears as inclusion bodies that require refolding to recover its biological activity. An important step following refolding is to determine the activity of refolded protein. Current methods for enzymatic activity of thrombolytic drugs are costly and complex. Here a straightforward and low-cost clot lysis assay was developed. It quantitatively measures the activity of the comm...
Published on Jul 21, 2014in PLOS ONE 2.78
Seiji Komatsu5
Estimated H-index: 5
,
Yusuke Nagai5
Estimated H-index: 5
(Okayama University)
+ 1 AuthorsYoshihiro Kimata21
Estimated H-index: 21
(Okayama University)
Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (n = 12/group). Secon...
Published on Nov 19, 2012in International Journal of Molecular Sciences 4.18
Ting Wang1
Estimated H-index: 1
,
Xiaozhong Zhong1
Estimated H-index: 1
+ 2 AuthorsXiaojun Zhao3
Estimated H-index: 3
(MIT: Massachusetts Institute of Technology)
Ionic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multiple tissues and in a variety of different wounds. In the present study, the nanofiber scaffolds of RADA16-1 peptide were sonicated into smaller fragments to identify possible molecular mechanisms underl...
Published on Oct 1, 2011in Journal of Food Biochemistry 1.36
A. Prathapan1
Estimated H-index: 1
(UoK: University of Kerala),
T. Rajamohan1
Estimated H-index: 1
(UoK: University of Kerala)
ABSTRACT Tender coconut water (TCW) is the sterile nutritious beverage that nature has provided. Due to the presence of biologically active nutrients, TCW possess beneficial effects in health and diseases. In the present study, effects of TCW on isoproterenol (ISO)-induced myocardial infarction in rats with respect to their antioxidant and antithrombotic effects have been investigated and were compared with that of streptokinase. Administration of ISO significantly decreased the activity of anti...
Alaaeldin I. Saleh1
Estimated H-index: 1
(German University in Cairo),
Sahar M. Abdel Maksoud1
Estimated H-index: 1
(German University in Cairo)
+ 1 AuthorsMohamed Z. Gad19
Estimated H-index: 19
(German University in Cairo)
Objective: Coronary artery diseases including myocardial ischemia (MI) remain one of the leading causes of death worldwide. This study was designed to compare the protective effect of L-arginine versus aspirin from the biochemical changes associated with MI injury. Experimental design: Four groups of male New Zealand white rabbits were investigated. Normal group (n ¼ 8) rabbits were fed standard chow pellets, untreated MI group (n ¼ 16), where hypercholesterolemia was induced by feeding the anim...
K.G. Nevin1
Estimated H-index: 1
(UoK: University of Kerala),
T. Rajamohan1
Estimated H-index: 1
(UoK: University of Kerala)
Summary Background aims Experimental and epidemiological studies indicate an association between dietary saturated fatty acids and thrombosis, but the effects of individual fatty acids on haemostasis are still controversial. The purpose of this research is to evaluate the effect of feeding virgin coconut oil (VCO) on blood coagulation factors, lipid levels and in vitro oxidation of LDL in comparison with copra oil (CO) and sunflower oil (SFO) in cholesterol (1%) and oil (10% w/w) fed rats. Metho...
Published on Sep 1, 2007in British Journal of Pharmacology 6.58
Stefan Chlopicki27
Estimated H-index: 27
(Jagiellonian University Medical College),
J Swies1
Estimated H-index: 1
(Jagiellonian University Medical College)
+ 5 AuthorsJerzy Gebicki15
Estimated H-index: 15
(University of Łódź)
Background and purpose: 1-methylnicotinamide (MNA) has been considered to be an inactive metabolite of nicotinamide. Here we assessed the anti-thrombotic activity of MNA in vivo. Experimental approach: Antithrombotic action of MNA was studied in normotensive rats with extracorporeal thrombus formation (thrombolysis), in renovascular hypertensive rats with intraarterial thrombus formation (arterial thrombosis) and in a venous thrombosis model in rats (venous thrombosis). Key results: MNA (3-100 m...