Incomplete Factorial Search for Conditions Leading to High Quality Crystals of Escherichia coli Cytidine Deaminase Complexed to a Transition State Analog Inhibitor
Abstract
We have used an incomplete factorial design (Carter, C. W., and Carter, C. W., Jr. (1979) J. Biol. Chem. 254, 12219-12223) to find conditions for growing high quality crystals of Escherichia coli cytidine deaminase (EC 3.5.4.5). Crystals grow at pH 6.0 in hanging or sitting drops with either 1.6 M ammonium sulfate or 2.4-2.5 M sodium phosphate as precipitant. Both conditions produce crystals with identical morphologies and unit cell constants....
Paper Details
Title
Incomplete Factorial Search for Conditions Leading to High Quality Crystals of Escherichia coli Cytidine Deaminase Complexed to a Transition State Analog Inhibitor
Published Date
Apr 1, 1989
Volume
264
Issue
12
Pages
6737 - 6740
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