Development and validation of an HPLC method to determine metabolites of 5-hydroxymethylfurfural (5-HMF)

The food component 5‐hydroxymethylfurfural is supposed to have antioxidative properties and is therefore used as an acting agent in a novel anticancer infusion solution, named K aral®, and an oral supplementation. Previous studies showed that after oral and intravenous application, the substance is completely decomposed to its metabolites: 5‐hydroxymethylfuroic acid, 2,5‐furandicarboxylic acid, and N ‐(hydroxymethyl)furoyl glycine. The formation...