Analysis of Telomeres and Telomerase
Abstract
This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also...
Paper Details
Title
Analysis of Telomeres and Telomerase
Published Date
Sep 1, 2003
Volume
20
Issue
1
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