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Cellular sources and inducers of cytokines present in acute wound fluid
Published on May 1, 2011in Wound Repair and Regeneration 2.95
· DOI :10.1111/j.1524-475X.2011.00668.x
Øystein Grimstad5
Estimated H-index: 5
(Norwegian University of Science and Technology),
Øystein Sandanger9
Estimated H-index: 9
(Norwegian University of Science and Technology)
+ 4 AuthorsTerje Espevik65
Estimated H-index: 65
(Norwegian University of Science and Technology)
Abstract
Acute wounds contain many biological active molecules, including several cytokines and growth factors. However, the cellular sources of each molecule, as well as the stimuli inducing them, are poorly characterized. We quantified the levels of 27 cytokines, chemokines, and growth factors in acute wound fluid in a luminex-based assay. The acute wound fluid contained particularly high levels of IL-6 and IL-8, as well as elevated levels of MCP-1, IL-1RA, PDGF, IP-10, IFN-γ, and TNF-α. Surprisingly, the amounts of IL-1β and IL-10 were relatively low. To characterize the cellular sources of these molecules, we analyzed supernatants from monocytes, neutrophils, keratinocytes, fibroblasts, and endothelial cells stimulated with pro- and anti inflammatory cytokines, and different Toll-like receptor (TLR) ligands. The different cell types showed overlapping but distinct patterns of production of signal molecules, as well as sensitivity to ligands. Among pro-inflammatory cytokines, IL-1β was the most potent inducer of signal molecule production. Furthermore, keratinocytes and endothelial cells were in particular responsive to the Toll-like receptor-3 ligand poly I:C. New interactions between cytokines and growth factors were revealed, which may have important roles in wound healing, including IL-1β-induced IFN-γ and IL-10-induced VEGF.
  • References (33)
  • Cited By (41)
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References33
Published on Apr 30, 2009
Christine Goldberg1
Estimated H-index: 1
,
Gordon Hall Gerould1
Estimated H-index: 1
The Grateful Dead. By Gordon Hall Gerould.. (Urbana and Chicago: University of Illinois Press, 2000; originally published in London by David Nutt for the Folklore Society, 1908. Pp. Xxxii + 177; introduction by Norm Cohen, chart, preface, index. $35.00 cloth, $15.00 paperback) "The Grateful Dead" is an intriguing title, but it does not tell the reader what to expect in this book. This title suggests folk beliefs and legends of ghosts and revenants, while this book is about a motif in certain fol...
9 Citations
Published on Feb 1, 2010in British Journal of Dermatology 6.13
Julius Renne10
Estimated H-index: 10
(Hannover Medical School),
V. Schäfer1
Estimated H-index: 1
(Hannover Medical School)
+ 1 AuthorsMiriam Wittmann24
Estimated H-index: 24
(Hannover Medical School)
Summary Background Chronic inflammatory skin diseases such as atopic dermatitis and psoriasis are characterized by the infiltration of lymphocytes into the epidermal compartment. Several studies point to an active role of skin epithelial cells in the pathophysiology of such diseases. Objectives In this study we addressed the regulatory function of primary human keratinocytes in the interaction with autologous T cells and monocytes. Methods We used a human coculture model with keratinocytes grown...
50 Citations Source Cite
Published on Feb 15, 1997in Journal of Immunology 4.54
Keizo Kohno21
Estimated H-index: 21
,
J Kataoka1
Estimated H-index: 1
+ 5 AuthorsMasashi Kurimoto69
Estimated H-index: 69
We have previously reported the cloning of a novel cytokine, IFN-gamma-inducing factor (IGIF), which shared some biologic activities with IL-12. In this study, we analyzed the effects of murine IGIF on the activation of T cells, and compared the effects with those of IL-12. IGIF alone had no effect on the activation of T cell lines or Th1 clones, while IGIF increased the IFN-gamma production by antigen-stimulated T cell lines, but had no effect on IL-4 or IL-10 production. As reported with IL-12...
476 Citations
Published on Dec 15, 1997in Journal of Immunology 4.54
H L Dickensheets1
Estimated H-index: 1
,
R P Donnelly1
Estimated H-index: 1
The Th2-type cytokines IL-4 and IL-13 induce expression of a distinct subset of genes in human monocytes. These include Fc epsilonRII (CD23), 15-lipoxygenase, IL-1 receptor antagonist (IL-1ra), and type I and type II IL-1 receptors (IL-1R). IFN-gamma has been shown to inhibit induction of CD23 and 15-lipoxygenase in monocytes; however, the effects of IFN-gamma on type I and type II IL-1R gene expression have not been defined. We examined the effects of IFN-gamma on both basal and IL-4/IL-13-indu...
62 Citations
Published on Jun 28, 2006in Wound Repair and Regeneration 2.95
Gaetano Di Vita14
Estimated H-index: 14
,
Rosalia Patti14
Estimated H-index: 14
+ 7 AuthorsEnrico Cillari23
Estimated H-index: 23
Knowing the dynamics of growth factor and cytokine secretion within the site of a surgical operation is important, as they play a crucial role in the pathophysiology of wound healing and are a target for modifying the repair response. The aim of this study was to evaluate the production of several cytokines and growth factors in the drainage wound fluid from patients undergoing incisional hernia repair: namely, interleukin (IL)-6, IL-10, IL-1α, IL-1 ra, interferon-y, vascular endothelial growth ...
31 Citations Source Cite
Published on Mar 1, 2008in International Journal of Legal Medicine 2.32
Masataka Takamiya17
Estimated H-index: 17
(Iwate Medical University),
Sachiko Fujita7
Estimated H-index: 7
(Iwate Medical University)
+ 1 AuthorsYasuhiro Aoki20
Estimated H-index: 20
(Iwate Medical University)
We performed quantification of IL 2, IL 4, IL 6, IL 8, IL 10, GM-CSF, IFN γ, and TNF α in human dermal wounds for wound age estimation. The proliferation of dermal cells and infiltration of inflammatory cells were also analyzed. Neutrophils and macrophages were detected from 2 h post-injury, and strong infiltrations were seen at 33–49 h. T and B lymphocytes also infiltrated simultaneously from 71 h. Strong proliferation of fibroblasts were shown from 246 h, and thickening of the epidermis from 7...
45 Citations Source Cite
Published on Sep 1, 1998in Annals of the New York Academy of Sciences 4.28
Lisa R. Leon14
Estimated H-index: 14
(Lovelace Respiratory Research Institute),
Wieslaw Kozak17
Estimated H-index: 17
(Lovelace Respiratory Research Institute),
Matthew J. Kluger15
Estimated H-index: 15
(Lovelace Respiratory Research Institute)
: Interleukin-10 (IL-10) inhibits the synthesis of proinflammatory cytokines known to be involved in fever, including IL-1, IL-6, and tumor necrosis factor-α. We hypothesized that IL-10 modulates lipopolysaccharide (LPS)-induced fever in mice. Body temperature was measured by biotelemetry. Swiss Webster mice injected with recombinant murine IL-10 (rmuIL-10) were resistant to fever induced by a low dose of LPS (100 μg/kg, ip) and to the hypothermic and febrile effects of a high (septic-like) dose...
41 Citations Source Cite
Mikiko Tohyama26
Estimated H-index: 26
(Ehime University),
Xiuju Dai16
Estimated H-index: 16
(Ehime University)
+ 9 AuthorsHiroshi Nagai5
Estimated H-index: 5
(Ehime University)
Abstract MIP-1α, a CC chemokine, recruits monocytes, natural killer cells, lymphocytes, and neutrophils, and plays a critical role in viral infection. Since, the lesional epidermis of herpes zoster expressed MIP-1α, we hypothesized that keratinocytes produce MIP-1α in response to virus-associated dsRNA via TLR3. To investigate this, we examined cultured human keratinocytes for MIP-1α production induced by poly(I:C), a TLR3 ligand. Poly(I:C) treatment induced MIP-1α production, interestingly, pol...
30 Citations Source Cite
Published on Sep 1, 2000in Journal of Surgical Research 2.05
Hans-Oliver Rennekampff2
Estimated H-index: 2
(University of California, San Diego),
John F. Hansbrough40
Estimated H-index: 40
(University of California, San Diego)
+ 3 AuthorsJens-Michael Schröder53
Estimated H-index: 53
(University of Kiel)
Abstract Background. Wound healing is a sequential biological process that involves the integration of chemotaxis of neutrophils, mitosis and migration of keratinocytes, and remodeling of the scar, all of which are regulated by specific soluble mediators. To modulate wound healing specific mediators have to be identified and functionally characterized. Therefore we addressed this study on the polymorphonuclear leukocyte (PMN) attractant interleukin-8 (IL-8) and its function in epidermal wound he...
194 Citations Source Cite
Published on Sep 1, 1992in Journal of Investigative Dermatology 6.45
Andrea Tuschil2
Estimated H-index: 2
,
Charles Lam9
Estimated H-index: 9
+ 1 AuthorsIvan J. D. Lindley24
Estimated H-index: 24
Abstract The presence of large amounts of biologically active interleukin-8 (IL-8) in psoriatic involved skin suggests that it may contribute, in part, to the changes observed in psoriasis, including hyperproliferation of keratinocytes. To examine the effect of IL-8 on epidermal growth, we monitored cytosolic free Ca ++ transients in human keratinocytes adult skin epidermis calcium reduced level, temperature elevated (HaCat) cells and normal keratinocytes loaded with the cell permeable, acetoxym...
183 Citations Source Cite
Cited By41
Published on Mar 1, 2011in Endodontic Topics
Lari Häkkinen34
Estimated H-index: 34
,
Hannu Larjava44
Estimated H-index: 44
,
Leeni Koivisto20
Estimated H-index: 20
The aim of this review is to summarize the key biological processes involved in soft connective tissue wound healing particularly in the context of oral mucosa. In a broader sense, healing of the connective tissue is not a separate, self-driven process, but occurs in a reciprocal interaction with other tissue components and cells, including the epithelium, vasculature and inflammatory cells, and these interactions will also be discussed when relevant.
18 Citations Source Cite
Published on Jan 1, 2013
S. L. M. van Loon1
Estimated H-index: 1
,
A. I. P. M. Smits1
Estimated H-index: 1
+ 2 AuthorsC. V. C. Bouten1
Estimated H-index: 1
The gold standard for treatment of advanced heart valve disease is surgical heart valve re‐ placement. None of the currently available mechanical and bioprosthetic heart valve substi‐ tutes resembles normal heart valve function. While mechanical heart valves offer excellent durability, they require life-long anticoagulation to control thromboembolism, which inher‐ ently leads to an increased risk of hemorrhage complications. Bioprosthetic valves on the other hand, retain a more physiological blo...
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Published on Jan 1, 2011
Markus Löffler1
Estimated H-index: 1
,
Michael Schmohl4
Estimated H-index: 4
+ 1 AuthorsStefan Beckert16
Estimated H-index: 16
Wound fluid seems -at least theoreticallyeasily accessible and might open a new window to the local wound microenvironment that cannot be evaluated by the analysis of serum or plasma markers. Recently, this strategy has been supported by a first time wound fluid proteome analysis comparing acute and chronic wounds (Eming et al. 2010). Interestingly, there seem to be essential differences with respect to wound fluid protein composition when comparing acute and chronic wounds. The wound fluid prot...
5 Citations Source Cite
Published on Aug 1, 2011
Schistosoma mansoni is a parasitic helminth which gains access to the host’s vascular system by penetrating and migrating through the skin in search of a blood vessel. The aim of this thesis was to determine whether during this migration the schistosome cercariae induce blood vessel growth (angiogenesis). This was examined following both a single exposure to the parasite (1x) and four exposures (4x). After 4x infections it has been shown that the skin immune response is predominantly Th2 and may...
Published on Jan 1, 2012in Experimental Dermatology 2.61
Øystein Grimstad5
Estimated H-index: 5
(Norwegian University of Science and Technology),
Brita Pukstad7
Estimated H-index: 7
(Norwegian University of Science and Technology)
+ 1 AuthorsTerje Espevik65
Estimated H-index: 65
(Norwegian University of Science and Technology)
6 Citations Source Cite
Published on Feb 1, 2016in Clinical Reviews in Allergy & Immunology 6.44
Ervin Ç. Mingomataj3
Estimated H-index: 3
,
Alketa H. Bakiri4
Estimated H-index: 4
The interleukin-10 (IL-10) is generally considered as the most important cytokine with anti-inflammatory properties and one of the key cytokines preventing inflammation-mediated tissue damage. In this respect, IL-10 producing cells play a crucial role in the outcome of infections, allergy, autoimmune reactions, tumor development, and transplant tolerance. Based on recent findings with regard to the mentioned clinical conditions, this review attempts to shed some light on the IL-10 functions, con...
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Markus W. Löffler8
Estimated H-index: 8
(University of Tübingen),
Heiko Schuster12
Estimated H-index: 12
(University of Tübingen)
+ 1 AuthorsStefan Beckert16
Estimated H-index: 16
(University of Tübingen)
Valid and reproducible sampling techniques as well as processing protocols are required for the assessment of biomarkers and mediators contained in wound exudate. Moreover, the ideal technique should be easy to use even in daily clinical routine. This is challenging since wound fluid represents an inhomogeneous mixture of different exogenous and endogenous sources. Analyzing wound fluid, however, may facilitate clinical decision making. Many techniques for obtaining wound fluid have been describ...
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Published on Aug 22, 2013in PLOS ONE 2.77
Takumi Yamane7
Estimated H-index: 7
(University of Tokyo),
Gojiro Nakagami18
Estimated H-index: 18
(University of Tokyo)
+ 6 AuthorsHiromi Sanada27
Estimated H-index: 27
(University of Tokyo)
Background Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin.
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Published on Jun 1, 2012in Burns 2.13
Matti Peura5
Estimated H-index: 5
(University of Helsinki),
Ilkka Kaartinen9
Estimated H-index: 9
+ 5 AuthorsJyrki Vuola15
Estimated H-index: 15
(Helsinki University Central Hospital)
Abstract Introduction Timely coverage of an excised burn wound with a split-thickness skin graft, and efficient epithelialization at the donor site wound are key components in the treatment of burn patients. Prompt healing is dependent on paracrine support from underlying dermal connective tissue fibroblasts. Study aim Using the skin graft donor site in pig as a model for epithelialization, our aim was to evaluate if dermal signals, derived from cultured dermal fibroblast aggregates (Finectra), ...
9 Citations Source Cite
Published on Dec 1, 2014in Journal of Reproductive Immunology 2.32
Line Haugstad Tangerås5
Estimated H-index: 5
(Norwegian University of Science and Technology),
Guro Stødle2
Estimated H-index: 2
(Norwegian University of Science and Technology)
+ 10 AuthorsMerete Fuglesang Myklebost1
Estimated H-index: 1
Abstract Toll-like receptors (TLRs) are an important part of the body's danger response system and crucial for initiating inflammation in response to cellular stress, tissue damage, and infections. Proper placental development is sensitive to inflammatory activation, and a role for TLRs in trophoblast immune activation has been suggested, but no overall examination has been performed in primary trophoblasts of early pregnancy. This study aimed to broadly examine cell surface and endosomal TLR ge...
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