A new logic for DNA engineering using recombination in Escherichia coli
Abstract
A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is transferable between E. coli strains. Several target molecules were manipulated, including high copy plasmids, a large episome and the E. coli chromosome. Sequential steps of homologous or site-specific recombination were used to demonstrate a new logic for engineering DNA, unlimited by the...
Paper Details
Title
A new logic for DNA engineering using recombination in Escherichia coli
Published Date
Oct 1, 1998
Journal
Volume
20
Issue
2
Pages
123 - 128
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