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  • References (213)
  • Citations (167)
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References213
Newest
Published on Aug 1, 1975in Archives of Biochemistry and Biophysics 3.56
Alexander McPherson4
Estimated H-index: 4
(Penn State Milton S. Hershey Medical Center),
Roderick Spencer1
Estimated H-index: 1
(MIT: Massachusetts Institute of Technology)
Abstract The plant seed protein canavalin has been crystallized alter trypsinization by using vapor diffusion to effect isoelectric precipitation. The space groups and cell dimensions are R3 with a = 81.3 A , γ = 111 ° and having extremely high R 32 pseudo symmetry, P6 3 with a = 126 A and c = 51 A , C222 1 , with a = 136 A , b = 152 A , and c = 131 A . X-ray data combined with electron microscopy suggests the molecule to be composed of six identical subunits. each of 18,500 daltons, arranged in...
Published on Oct 1, 1974in Nature 43.07
Carolyn Cohen47
Estimated H-index: 47
(Brandeis University),
Nancy M. Tooney5
Estimated H-index: 5
MOST fibrous proteins do not crystallise. An artful way to promote protein crystallisation is to modify the structure by proteolytic attack. For example, papain cleavage of γ-globulin yields the homogeneous Fc fragment which readily crystallises1. Following a similar approach, we have crystallised fibrinogen.
Published on Oct 1, 1974in Nature 43.07
G. Zaloga1
Estimated H-index: 1
(SBU: Stony Brook University),
R. Sarma1
Estimated H-index: 1
(SBU: Stony Brook University)
A CONSIDERABLE problem hampering the analysis of protein crystal structures has been the damage caused to the crystals by the X-ray beam. Another limiting factor has been the resolution in the diffraction pattern. To increase the useful life of a protein crystal in the X-ray beam1, single crystal monochromators and low temperatures have been used. We now report that styrene and other vinyl monomers can decrease the radiation damage of the crystal in the X-ray beam by a factor of as much as 10, a...
Published on Sep 1, 1974in Journal of Molecular Biology 5.07
A. C. T. North2
Estimated H-index: 2
,
Gerald Stubbs24
Estimated H-index: 24
Abstract X-ray crystallographic studies of hemerythrin from Golfingia gouldii show that the molecules pack in a tetragonal cell with two sets of molecules in an apparently face-centred array seen in projection along the 4-fold axis, but displaced relatively to each other by approximately c 10 in the axial direction. Both sets of molecules lie on 4-fold rotation axes, so that the subunits of each octameric molecule are related in pairs by a molecular 4-fold rotation axis. The two subunits of each...
Published on Sep 1, 1974in Journal of Molecular Biology 5.07
John L. Wang8
Estimated H-index: 8
(Rockefeller University),
Joseph W. Becker5
Estimated H-index: 5
(Rockefeller University)
+ 1 AuthorsGerald M. Edelman130
Estimated H-index: 130
(Rockefeller University)
Abstract A lectin from the fava bean ( Vicia faba ) has been purified and crystallized in a form suitable for high-resolution crystallographic structure analysis. This protein binds glucose- and mannose-like saccharides, and it is mitogenic for lymphocytes. The fava lectin crystallizes in the orthorhombic space group. P 2 1 2 1 2 1 with unit cell dimensions a = 90.0, b = 89.3, and c = 67.4 A . The mass of protein in the asymmetric unit is 53,000 daltons, corresponding to the molecular weight of ...
Published on Aug 2, 1974in Science 41.04
Sung-Hou Kim83
Estimated H-index: 83
(Duke University),
F. L. Suddath12
Estimated H-index: 12
(MIT: Massachusetts Institute of Technology)
+ 5 AuthorsAlexander Rich100
Estimated H-index: 100
(MIT: Massachusetts Institute of Technology)
The 3-angstrom electron density map of crystalline yeast phenylalanine transfer RNA has provided us with a complete three-dimensional model which defines the positions of all of the nucleotide residues in the moleclule. The overall features of the molecule are virtually the same as those seen at a resolution of 4 angstroms except that many additional details of tertiary structure are now visualized. Ten types of hydrogen bonding are identified which define the specificity of tertiary interaction...
Published on Aug 1, 1974in Journal of Molecular Biology 5.07
Christine Schubert Wright6
Estimated H-index: 6
(Princeton University),
C. Keith1
Estimated H-index: 1
(Princeton University)
+ 2 AuthorsM.M. Burger1
Estimated H-index: 1
(University of Basel)
Abstract Wheat germ agglutinin crystallizes in two monoclinic space groups, P 2 1 and C 2, under identical crystallization conditions. Unit cell dimensions are a = 73.8 A , b = 51.2 A , c = 90.8 A , γ = 90 ° for P2 1 ; a = 51.31 A , b = 73.35 A , c = 91.45 A , β = 97.75 ° for C 2, both with eight subunit molecules in the unit cell. The C 2 crystals were chosen as suitable for investigating the three-dimensional structure to high resolution, because of their smaller asymmetric unit (containing th...
Published on Aug 1, 1974in Nature 43.07
Jon D Robertus6
Estimated H-index: 6
,
Jane E. Ladner16
Estimated H-index: 16
(Medical Research Council)
+ 4 AuthorsAaron Klug7
Estimated H-index: 7
(Medical Research Council)
The structure of a tRNA has been determined by isomorphous replacement. Some of the interactions which maintain the tertiary structure are of a novel type. Our model differs significantly from one which has recently been proposed.
Published on Aug 1, 1974in Journal of Molecular Biology 5.07
Bi-Cheng Wang47
Estimated H-index: 47
,
M. Sax21
Estimated H-index: 21
Abstract A Bence-Jones protein, Rhe, isolated from the urine of a myeloma patient, has been crystallized. Immunological tests indicate that Rhe contains the constant region of a lambda-type light chain. Gel filtration and sodium dodecyl sulfate gel electrophoresis show that Rhe consists of two subunits, each about half the size of the light chain. Preliminary X-ray crystallographic results and density measurements show that Rhe possesses crystallographic 2-fold symmetry, indicating that the two ...
Published on Jul 25, 1974in Journal of Biological Chemistry
Gerard J. Bunick3
Estimated H-index: 3
,
George P. McKenna1
Estimated H-index: 1
+ 1 AuthorsDonald Voet5
Estimated H-index: 5
Abstract Single crystals of the yeast enzyme inorganic pyrophosphatase that are suitable for x-ray analysis have been grown. The space group of the crystals is P21. Their unit cell dimensions are: a = 70.4, b = 95.4, c = 52.2 A, and β = 99.8°. There is 1 dimeric enzyme molecule with a molecular weight of approximately 68,000 in the asymmetric unit of the unit cell. The crystal is approximately 57% protein by weight.
Cited By167
Newest
Published on Dec 1, 2018in Heliyon
Takayuki Odahara5
Estimated H-index: 5
(AIST: National Institute of Advanced Industrial Science and Technology),
Koji Odahara1
Estimated H-index: 1
Abstract Salt/polyethylene glycol (PEG) mixtures are employed as precipitants for biological macromolecules. The dependence of precipitation curves (PCs) on salt species was investigated for integral membrane protein/detergent particles. By relating this dependence to properties of ions dissociated from added salts, the following roles and effects of various ions were clarified. In the presence of ions whose interaction with water is stronger than water–water interaction, the coordination of sol...
Published on Jan 1, 2018
Claudia Fasolato (University of Perugia)
An interesting approach to the study of matter is represented by spectroscopy, that infers information about materials and biosystems by driving their interaction with controlled probes, as beams of light, electrons and neutrons. In this chapter, the basic physics of Raman and Surface Enhanced Raman Scattering (SERS) will be discussed. Furthermore, we will present a review of the scientific literature concerning the themes that are relevant for the Thesis, and in particular the fabrication of na...
Published on Jan 1, 2017in Methods in Enzymology 1.86
Jacob H. Artz4
Estimated H-index: 4
(WSU: Washington State University),
Oleg A. Zadvornyy8
Estimated H-index: 8
(WSU: Washington State University)
+ 2 AuthorsJohn W. Peters48
Estimated H-index: 48
(WSU: Washington State University)
Abstract The crystallization of FeS cluster-containing proteins has been challenging due to their oxygen sensitivity, and yet these enzymes are involved in many critical catalytic reactions. The last few years have seen a wealth of innovative experiments designed to elucidate not just structural but mechanistic insights into FeS cluster enzymes. Here, we focus on the crystallization of hydrogenases, which catalyze the reversible reduction of protons to hydrogen, and nitrogenases, which reduce di...
Published on May 1, 2016
Hilary P. Stevenson9
Estimated H-index: 9
(University of Pittsburgh),
Guowu Lin5
Estimated H-index: 5
(University of Pittsburgh)
+ 19 AuthorsAlexander M. Makhov17
Estimated H-index: 17
(University of Pittsburgh)
The crystallization of protein samples remains the most significant challenge in structure determination by X-ray crystallography. Here, the effectiveness of transmission electron microscopy (TEM) analysis to aid in the crystallization of biological macromolecules is demonstrated. It was found that the presence of well ordered lattices with higher order Bragg spots, revealed by Fourier analysis of TEM images, is a good predictor of diffraction-quality crystals. Moreover, the use of TEM allowed (...
Published on Apr 1, 2016in Protein Expression and Purification 1.29
Takayuki Odahara5
Estimated H-index: 5
(AIST: National Institute of Advanced Industrial Science and Technology),
Koji Odahara1
Estimated H-index: 1
Mixtures of neutral salts and polyethylene glycol are used for various purposes in biological studies. Although the effects of each component of the mixtures are theoretically well investigated, comprehension of their integrated effects remains insufficient. In this work, their roles and effects as a precipitant were clarified by studying dependence of precipitation curves on salt concentration for integral membrane protein/detergent particles of different physicochemical properties. The depende...
Published on Oct 28, 2015in Micromachines 2.43
Deyong Zhu2
Estimated H-index: 2
,
Xiaohu Zhou1
Estimated H-index: 1
,
Bo Zheng1
Estimated H-index: 1
This paper reports the design and construction of a plastic-glass hybrid microfluidic platform for performing protein crystallization trials in nanoliter double emulsions. The double emulsion-based protein crystallization trials were implemented with both the vapor-diffusion method and microbatch method by controlling the diffusion of water between the inner and outer phases and by eliminating water evaporation. Double emulsions, whose inner and outer environments can be easily adjusted, can pro...
Published on Jan 1, 2015in Methods of Molecular Biology
Vincent J. Fazio7
Estimated H-index: 7
(CSIRO: Commonwealth Scientific and Industrial Research Organisation),
Thomas S. Peat22
Estimated H-index: 22
(CSIRO: Commonwealth Scientific and Industrial Research Organisation),
Janet Newman26
Estimated H-index: 26
(CSIRO: Commonwealth Scientific and Industrial Research Organisation)
Published on Oct 31, 2014in Journal of Biological Chemistry
Geoffrey K. Feld3
Estimated H-index: 3
,
Sahar El-Etr2
Estimated H-index: 2
+ 6 AuthorsAmy Rasley7
Estimated H-index: 7
Francisella tularensis is the etiological agent of tularemia, or rabbit fever. Although F. tularensis is a recognized biothreat agent with broad and expanding geographical range, its mechanism of infection and environmental persistence remain poorly understood. Previously, we identified seven F. tularensis proteins that induce a rapid encystment phenotype (REP) in the free-living amoeba, Acanthamoeba castellanii. Encystment is essential to the pathogen's long term intracellular survival in the a...
Joseph R. Luft28
Estimated H-index: 28
(Hauptman-Woodward Medical Research Institute),
Janet Newman26
Estimated H-index: 26
(CSIRO: Commonwealth Scientific and Industrial Research Organisation),
Edward H. Snell23
Estimated H-index: 23
(UB: University at Buffalo)
While crystallization historically predates crystallography, it is a critical step for the crystallographic process. The rich history of crystallization and how that history influences current practices is described. The tremendous impact of crystallization screens on the field is discussed.
Hilary P. Stevenson9
Estimated H-index: 9
(University of Pittsburgh),
Alexander M. Makhov17
Estimated H-index: 17
(University of Pittsburgh)
+ 13 AuthorsTed M. Ross22
Estimated H-index: 22
The current practice for identifying crystal hits for X-ray crystallography relies on optical microscopy techniques that are limited to detecting crystals no smaller than 5 μm. Because of these limitations, nanometer-sized protein crystals cannot be distinguished from common amorphous precipitates, and therefore go unnoticed during screening. These crystals would be ideal candidates for further optimization or for femtosecond X-ray protein nanocrystallography. The latter technique offers the pos...