Activity of Some Plant Extracts Against Multi-Drug Resistant Human Pathogens

Published on Jan 1, 2009in Iranian Journal of Pharmaceutical Research1.183
· DOI :10.22037/ijpr.2010.825
Mustafa Oskay10
Estimated H-index: 10
(Celal Bayar University),
Dilek Oskay2
Estimated H-index: 2
Fatih Kalyoncu11
Estimated H-index: 11
Plants used for traditional medicine contain a wide range of substances which can be used to treat various infectious diseases. Hence, antibacterial activities of ethanolic extracts of 19 plant species were studied against multi-drug resistant clinical isolates using agar well diffusion method. Extracts of Liquidambar orientalis , Vitis vinifera , Rosmarinus officinalis , Punica granatum , Cornus sanguinea , Euphorbia peplus , Ecballium elaterium , Inula viscosa and Liquidambar orientalis showed broad-spectrum antibacterial activity with inhibition zones ranging from 8 to 26 mm. The most resistant organisms were Escherichia coli ( E. coli ) (Ampicillin-, amoxycillin- and sulfamethoxazole-resistant), Stenotrophomonas maltophilia ( S. maltophilia ) (Amoxycillin- and nalidixic acid-resistant) and Klebsiella pneumoniae ( K. pneumoniae ) (Ampicillin-, amoxycillin- and aztreonam-resistant), and the most susceptible species were Staphylococcus aureus ( S. aureus ) (Penicillin G- and oxacillin-resistant), Streptococcus pyogenes ( S. pyogenes ) (Penicillin G-, erythromycin- and clindamycin-resistant) and Pseudomonas aeruginosa ( P. aeruginosa ) (Sulfamethoxazole- and novobiocin-resistant), respectively. Minimum Inhibitory Concentrations (MIC) of crude extracts were determined for the seven highly active plants showing activity against methicillin resistant S. aureus (MRSA), E. coli , P. aeruginosa , S. pneumoniae and the reference bacteria ( E. coli ATCC 11229 and Kocuria rhizophila ATCC 9341 NA). MICs of active extracts ranged from 8 to 14.2 mg/mL against one or other test bacteria.
  • References (13)
  • Citations (30)
📖 Papers frequently viewed together
3 Authors (Mustafa Oskay, ..., Fatih Kalyoncu)
11.2k Citations
110 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Mustafa Oskay (Celal Bayar University)H-Index: 10
#2Dilek Sari (Celal Bayar University)H-Index: 3
AbstractEthanol extracts of 19 Turkish medicinal plants, used in the traditional system of medicine, were investigated for their antimicrobial activity against 14 pathogenic bacterial species and a yeast, Candida albicans., using the agar well diffusion method. Anticandidal activity was detected in 10 plant extracts. Extracts of Eucalyptus camuldulensis. (leaves), Rosmarinus officinalis. (leaves), Ecballium elaterium. (leaves, fruits; 2:1, v/v), Liquidambar orientalis. (leaves), Cornus sanguinea...
42 CitationsSource
#1Ergin Uzun (Istanbul University)H-Index: 1
#2Günay Sariyar (Istanbul University)H-Index: 9
Last. Sevda Pırıldar (Istanbul University)H-Index: 4
view all 7 authors...
Abstract Traditional medicine in North–West of Turkey (Sakarya province) were studied during a 2 months field study by interviewing local informants from several villages. Plant species used to treat infections were tested for antimicrobial activity. Information was collected for 46 plant species from 30 families and for 5 animal species. Twenty four of the plant species were cultivated. Most used families were Asteraceae, Cucurbitaceae, Lamiaceae and Rosaceae and the most used plants were Artem...
129 CitationsSource
80 CitationsSource
Tribulus terrestris is used as a urinary anti-infective in folk medicine. To validate this use, the in vitro antibacterial activity of methanolic extracts of different parts (fruits, stems plus leaves and roots) of T. terrestris L. growing in Iran was evaluated against four reference bacteria by broth dilution assay and agar diffusion assay. The MIC value of the methanolic extracts of fruits and stems plus leaves against all bacteria was 2 mg/mL and the MIC value of roots against S. aureus, E. f...
50 Citations
#1Özlem Turgay (Kahramanmaraş Sütçü İmam University)H-Index: 2
The antibacterial activities of ethyl acetate, methanol, chloroform, and acetone extracts of four plant species were studied. The dried extracts of the whole plant of Artemisia absinthium (Compositae/Asteraceae) and Urtica dioica (Urticaceae), flowering plants of Fumaria officinalis (Papaveraceae/Fumariaceae) and the leaves of Rosmarinus officinalis (Labiatae/Lamiaceae) were tested in vitro against 12 bacterial species and strains by the agar diffusion method. Bacillus brevis FMC 3, Bacillus meg...
151 CitationsSource
24 CitationsSource
#1Iqbal Ahmad (AMU: Aligarh Muslim University)H-Index: 57
#2Arina Z. Beg (AMU: Aligarh Muslim University)H-Index: 3
Ethanolic extracts of 45 Indian medicinal plants traditionally used in medicine were studied for their antimicrobial activity against certain drug-resistant bacteria and a yeast Candida albicans of clinical origin. Of these, 40 plant extracts showed varied levels of antimicrobial activity against one or more test bacteria. Anticandidal activity was detected in 24 plant extracts. Overall, broad-spectrum antimicrobial activity was observed in 12 plants (L. inermis, Eucalyptus sp., H. antidysentric...
821 CitationsSource
#1Laila F. Nimri (JUST: Jordan University of Science and Technology)H-Index: 18
#2M. M. Meqdam (JUST: Jordan University of Science and Technology)H-Index: 10
Last. Ahmad S. Alkofahi (JUST: Jordan University of Science and Technology)H-Index: 21
view all 3 authors...
The antibacterial activity of ethanol extracts of 15 plant species used in the traditional medicine in Jordan and other Middle East countries were tested. Extracts of certain parts of these plants were tested in vitro against 14 pathogenic bacterial species and strains using the agar diffusion method. Results evaluated as the diameter of inhibition zone of bacterial growth showed that 25 mg/well of 12 plant extracts have antibacterial activity on one or more of the tested bacteria. Three plants ...
109 CitationsSource
#1Michael A. Pfaller (UI: University of Iowa)H-Index: 106
#2Ronald N. Jones (UI: University of Iowa)H-Index: 99
Last. Kari C. Kugler (UI: University of Iowa)H-Index: 22
view all 4 authors...
The SENTRY Program was established in January 1997 to measure the predominant pathogens and antimicrobial resistance patterns of nosocomial and community-acquired infections over a broad network of sentinel hospitals in the United States (30 sites), Canada (8 sites), South America (10 sites), and Europe (24 sites). During the first 6-month study period (January to June 1997), a total of 5,058 bloodstream infections (BSI) were reported by North American SENTRY participants (4,119 from the United ...
497 CitationsSource
Freeze dried and finely ground leaves of two plants with known antimicrobial activity, Anthocleista grandiflora and Combretum erythrophyllum were extracted with acetone, ethanol, methanol, methylenedichloride, methanol/chloroform/water and water at a 1 to 10 ratio in each case. The quantity and diversity of compounds extracted, number of inhibitors extracted, rate of extraction, toxicity in a bioassay, ease of removal of solvent and biological hazard were evaluated for each extractant. An arbitr...
732 CitationsSource
Cited By30
#2Nahla E.M. IsmaelH-Index: 1
Last. Adham A. Al-SagheerH-Index: 5
view all 5 authors...
Abstract The objective of the existing trial was to examine the effect of enrichment of tilapia (Oreochromis niloticus) diets with rosemary leaf powder (RLP) on growth, antioxidant defense parameters, immune function indicators, and disease resistance. Nile tilapia fingerlings (10.42 ± 0.04 g) were fed four diets supplemented with RLP at 0, 2.5, 5, and 10 g kg−1 diet for 60 days. At the end of the feeding trial, fish were challenged with Aeromonas hydrophila for 14 days, and the mortality rate w...
#1Laura Grauso (University of Naples Federico II)H-Index: 2
#2Gaspare Cesarano (University of Naples Federico II)H-Index: 8
Last. Virginia Lanzotti (University of Naples Federico II)H-Index: 29
view all 7 authors...
Dittrichia viscosa is a perennial small shrub belonging to the Compositae family (Asteraceae) widespread in the Mediterranean basin. This plant has been extensively used in traditional medicine since the Roman times as reported by the Roman Naturalist Gaius Plinius Secundus. Nowadays, many studies about chemical composition and biological activity of D. viscosa are available. Chemical analyses of plant extracts revealed the presence of several metabolites belonging to different classes of natura...
4 CitationsSource
#1Abdelhakim BouyahyaH-Index: 10
#2Abdeslam Et-TouysH-Index: 8
Last. Youssef BakriH-Index: 14
view all 8 authors...
Inula viscosa (L.) (synonyme de Dittrichia viscosa (L.) Greuter) est une plante medicinale tres utilisee dans la medecine populaire pour soigner differentes pathologies. Elle appartient a la famille des Asteraceae et est largement repandue au Maroc et dans le monde. Les etudes effectuees sur cette plante ont montre qu’elle est riche en metabolites secondaires tels que les acides phenoliques, les flavonoides et les composes terpenoides. Ces composes sont doues de proprietes antibacteriennes, anti...
Axenic cultures of Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus obtained from the Medical Microbiology Laboratory of the Delta State University Teaching Hospital, Oghara, were inoculated into ten-fold serial dilutions of 10-1 to 10-7 in sterile Nutrient broth and incubated in water bath at 37°C for 18 h initially. The turbid broth cultures of 10-5, 10-6 and 10-7 dilutions were subcultured aseptically on sterile MacConkey agar, Nutrient agar and Blood agar plates and incubate...
#1Daljit Singh Arora (G.N.D.U.: Guru Nanak Dev University)H-Index: 25
#2Henna Sood (G.N.D.U.: Guru Nanak Dev University)H-Index: 5
The in vitro antimicrobial screening of Gymnema sylvestre leaves against 13 test pathogens established its broad spectrum activity with average inhibition zone ranging from 14 to 23 mm. The antimicrobial activity of the classically- optimized aqueous extract was enhanced up to 1.45 folds, when subjected to statistical optimization using Response Surface Methodology (RSM) and was thermostable. Ethyl acetate was found to be the best organic extractant with Klebsiella pneumoniae 1 (31.5 mm) and Sta...
5 CitationsSource
#1Sherif T. S. Hassan (VFU: University of Veterinary and Pharmaceutical Sciences Brno)H-Index: 7
#2Kateřina Berchová-Bímová (CULS: Czech University of Life Sciences Prague)H-Index: 4
Last. K.T.S. Hassan (Alexandria University)H-Index: 1
view all 4 authors...
Abstract The search for biologically promising compounds from natural sources against microbial diseases remains an important theme in drug discovery to overcome problems with drug-resistant strains. In this study, Cucurbitacin B (Cuc B), an active constituent of Ecballium elaterium L., has been investigated in vitro for its synergy effect with antibiotics against clinical isolates of Staphylococcus aureus ( S. aureus ) and anti-HSV-1 activity. Broth microdilution method was used to determine th...
3 CitationsSource
The aim of the present study is to examine the antibacterial, antifungal and anti-inflammatory activities of the ethanolic leaf extract of Melia azedarach . It was tested in vitro for its antibacterial and antifungal activities against E. coli (ATCC 8739), Enterococcus faecalis (Ec P07) and Bacillus subtilis (Bs) bacteria as well as against pathogenic fungi ( Alternaria alternate , Fusarium solani, Fusarium oxysporum sp. melonis, F. oxysporum f. sp. lycopersici , F. sambucinum and Botrytis ciner...
1 CitationsSource
#1Hêro F.S Akrayi (Salahaddin University)H-Index: 1
#2Rebwar M.H. Salih (Salahaddin University)H-Index: 1
Last. Pishtiwan A. Hamad (Salahaddin University)H-Index: 1
view all 3 authors...
This study investigates the antibacterial property of Rhus coriaria (Sumac) and Origanum vulgare (Jatra) aqueous extracts against Escherichia coli ATCC 25922, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa ATCC 27835. Results confirm the resistance of the bacterial isolates against more than three antibiotics. The aqueous extract of R. coriaria showed the highest activity as an inhibitor against tested bacteria, while the aqueous extract of O. vulgare shows low effect agains...
3 CitationsSource
#1Mohammad ZandiH-Index: 2
#2Fatemeh JasourH-Index: 1
Last. Mohammad Reza SanjabiH-Index: 2
view all 4 authors...
Background: Herbal extracts have recently received the greatest attention in the path of finding naturally occurring chemicals with antibacterial and therapeutic value; however, each type of herbal remedy may have its own side effects. Objectives: The aim of the current experiment was to study the antibacterial effect of myrtle, parsley, mint, henna and chamomile extracts on Escherichia coli and their effects on colony formation and survival of spermatogonial stem cells (SSCs). Materials and Met...
1 CitationsSource
This research effort seeks to use doses of thioridaxine to enhance antibiotic sensitivity in a MDR Escherichia coli strain. Five axenic (pure) strains of Escherichia coli coded EC1 to EC5 were obtained from five infected midstream urine samples among several other urine samples inoculated on sterile Cystine Lactose Electrolyte Deficient (CLED) agar with appropriate labeling in the Microbiology and Biotechnology Laboratory of Western Delta University, Oghara, Nigeria and stocked on sterile Nutrie...