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Relative effectiveness of various ions on the solubility and crystal growth of lysozyme.

Published on Jan 15, 1989in Journal of Biological Chemistry
M M Ries-Kautt1
Estimated H-index: 1
,
A F Ducruix1
Estimated H-index: 1
Abstract
Abstract Crystallization conditions for hen egg white lysozyme in the presence of various ions were determined at pH 4.5 and 18 degrees C. The corresponding solubility curves show that the main effects are due to anions in the following order: SCN- greater than NO3- greater than Cl- greater than citrate2- greater than CH3COO- approximately H2PO4- greater than SO4(2-). This is in the reverse order of the lyotropic series of Hofmeister. As a consequence, SCN- precipitates and crystallizes lysozyme at low concentration, whereas sulfate is ineffective even at high concentrations. Crystals obtained with each salt were characterized by x-ray diffraction. Lysozyme thiocyanate and nitrate crystals belong to the monoclinic system, whereas all the others have a tetragonal lattice.
  • References (1)
  • Citations (255)
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The structure of orthorhombic lysozyme has been obtained at 298 K and pH 4.5 using sodium chloride as the precipitant and in the presence of sodium phosphate at a concentration as low as 5 mM. Crystals belonging to space group P212121 (unit-cell parameters a = 30, b = 56, c = 73 A, α = β = γ = 90.00°) diffracted to a resolution higher than 1 A, and the high quality of these crystals permitted the identification of a phosphate ion bound to Arg14 and His15. The binding of this ion produces long-ra...
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Abstract Differential scanning calorimetry (DSC) has been used to investigate the role that cations of different salts (CaCl 2 , MgCl 2 , NH 4 Cl, NaCl and KCl) play on the thermodynamic stability and reversibility of the lysozyme’s folding-unfolding process. In this work, thermodynamic parameters such as enthalpy change ΔΗ, apparent heat capacity change (ΔC p ) and the melt transition temperature (T m ) were obtained in order to explore the effects of various cations on the thermodynamic stabil...
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