Crystallization of Biological Macromolecules

Published on Aug 1, 1999
Alexander McPherson53
Estimated H-index: 53
The history and character of macromolecular crystals principles of macromolecular structure and the applications of x-ray crystallography the purification and characterization of biological macromolecules some physical and energetic principles practical procedures for macromolecular crystallization important considerations in macromolecular crystallization strategies and special approaches in growing crystals impurities, defects, and crystal quality the mechanisms and kinetics of macromolecular crystal growth crystal growth in unique environments.
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  • Citations (641)
Cited By641
Published on Feb 28, 2019in Bioscience Reports2.54
Almudena Ponce-Salvatierra1
Estimated H-index: 1
(International Institute of Minnesota),
Estimated H-index: 2
(International Institute of Minnesota)
+ 4 AuthorsJanusz M. Bujnicki55
Estimated H-index: 55
(Adam Mickiewicz University in Poznań)
Ribonucleic acid (RNA) molecules are master regulators of cells. They are involved in a variety of molecular processes: they transmit genetic information, sense cellular signals and communicate responses, and even catalyze chemical reactions. As in the case of proteins, RNA function is dictated by its structure and by its ability to adopt different conformations, which in turn is encoded in the sequence. Experimental determination of high-resolution RNA structures is both laborious and difficult...
Published on Feb 25, 2019in Pure and Applied Chemistry2.35
Sara Linse55
Estimated H-index: 55
Inhibition of amyloid β peptide (Aβ) aggregation is an important goal due to the connection of this process with Alzheimer's disease. Traditionally, inhibitors were developed with an aim to retard the overall macroscopic aggregation. However, recent advances imply that approaches based on mechanistic insights may be more powerful. In such approaches, the microscopic steps underlying the aggregation process are identified, and it is established which of these step(s) lead to neurotoxicity. Inhibi...
Published on Feb 1, 2019in Journal of energy storage
Shadi Hosseini (Urmia University), Khalil Farhadi24
Estimated H-index: 24
(Urmia University),
Sepideh Banisaeid2
Estimated H-index: 2
(Urmia University)
Abstract Present work proposes a unique and simple method for synthesizing small and uniform Barium sulfate particles within a novel auxiliary solution based on the glycerol. Glycerol acts as a molecular spacer and prevents growth and agglomeration of the synthesizing particles. Barium sulfate is the isomorph of PbSO 4 and provides the centers for nucleation of PbSO 4 particles during the discharge process. Smaller particles of BaSO 4 leads to more and smaller PbSO 4 particles per volume of Nega...
Published on Jan 1, 2019
Published on Dec 10, 2018in Journal of Biomolecular Structure & Dynamics3.31
Abhishikha Srivastava5
Estimated H-index: 5
(DU: University of Delhi),
Manish Kumar20
Estimated H-index: 20
(DU: University of Delhi)
Zinc is one the most abundant catalytic cofactor and also an important structural component of a large number of metallo-proteins. Hence prediction of zinc metal binding sites in proteins can be a significant step in annotation of molecular function of a large number of proteins. Majority of existing methods for zinc-binding site predictions are based on a data-set of proteins, which has been compiled nearly a decade ago. Hence there is a need to develop zinc-binding site prediction system using...
Published on Oct 1, 2018
Aleksandar Bijelic12
Estimated H-index: 12
(University of Vienna),
Annette Rompel29
Estimated H-index: 29
(University of Vienna)
Protein crystallography is the most widely used method for determining the molecular structure of proteins and obtaining structural information on protein–ligand complexes at the atomic level. As the structure determines the functions and properties of a protein, crystallography is of immense importance for nearly all research fields related to biochemistry. However, protein crystallography suffers from some major drawbacks, whereby the unpredictability of the crystallization process represents ...
Alexander McPherson53
Estimated H-index: 53
(UCI: University of California, Irvine),
Steven B. Larson13
Estimated H-index: 13
(UCI: University of California, Irvine)
It was found that the crystals of at least a dozen different proteins could be thoroughly stained to an intense color with a panel of dyes. Many, if not most, of the stained protein crystals retained the dyes almost indefinitely when placed in large volumes of dye-free mother liquor. Dialysis experiments showed that most of the dyes that were retained in crystals also bound to the protein when free in solution; less frequently, some dyes bound only in the crystal. The experiments indicated a str...
Published on Sep 1, 2018in Acta Materialia7.29
Ryo Suzuki3
Estimated H-index: 3
(YCU: Yokohama City University),
Masaru Tachibana18
Estimated H-index: 18
(YCU: Yokohama City University)
+ 1 AuthorsKenichi Kojima7
Estimated H-index: 7
(Yokohama Soei University)
Abstract Stress-induced dislocations in glucose isomerase (GI) crystals were investigated by synchrotron X-ray topography with simple indentation method. It is observed that the indentation gives rise to the deformation associated with strain and dislocations over the entire crystal. It is characterized that three kinds of mobile dislocations with Burgers vectors of 1 2 " is="true"> 111 , " is="true"> 100 and " is="true"> 010 are at least introduced by the impact stresses due to the indentation....
Kochise Bennett9
Estimated H-index: 9
(UCI: University of California, Irvine),
Markus Kowalewski11
Estimated H-index: 11
(UCI: University of California, Irvine)
+ 1 AuthorsShaul Mukamel76
Estimated H-index: 76
(UCI: University of California, Irvine)
Ultrafast time-resolved X-ray scattering, made possible by free-electron laser sources, provides a wealth of information about electronic and nuclear dynamical processes in molecules. The technique provides stroboscopic snapshots of the time-dependent electronic charge density traditionally used in structure determination and reflects the interplay of elastic and inelastic processes, nonadiabatic dynamics, and electronic populations and coherences. The various contributions to ultrafast off-reso...
Published on Jun 20, 2018in PLOS ONE2.78
Andrew E. Bruno8
Estimated H-index: 8
(UB: University at Buffalo),
Patrick Charbonneau24
Estimated H-index: 24
(Duke University)
+ 6 AuthorsJulie Wilson23
Estimated H-index: 23
(Ebor: University of York)
The Machine Recognition of Crystallization Outcomes (MARCO) initiative has assembled roughly half a million annotated images of macromolecular crystallization experiments from various sources and setups. Here, state-of-the-art machine learning algorithms are trained and tested on different parts of this data set. We find that more than 94% of the test images can be correctly labeled, irrespective of their experimental origin. Because crystal recognition is key to high-density screening and the s...
View next paperProcessing of X-ray diffraction data collected in oscillation mode