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Crystallization of Biological Macromolecules

Published on Jan 15, 1999
Alexander McPherson51
Estimated H-index: 51
Abstract
The history and character of macromolecular crystals principles of macromolecular structure and the applications of x-ray crystallography the purification and characterization of biological macromolecules some physical and energetic principles practical procedures for macromolecular crystallization important considerations in macromolecular crystallization strategies and special approaches in growing crystals impurities, defects, and crystal quality the mechanisms and kinetics of macromolecular crystal growth crystal growth in unique environments.
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  • Citations (650)
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Antibody therapeutics are one of the most important classes of drugs. Antibody structures have become an integral part of predicting the behavior of potential therapeutics, either directly or as the basis of modeling. Structures of Fab:antigen complexes have even greater value. While the crystallization and structure determination of Fabs is easy relative to many other protein classes, especially membrane proteins, broad screening and optimization of crystalline hits is still necessary. Through ...
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Abstract The enzyme Urocanate Hydratase (UH) participates in the catabolic pathway of L-histidine. Trypanosoma cruzi Urocanate Hydratase (TcUH) is identified as a therapeutic molecular target in the WHO/TDR Targets Database. We report the 3D structure determination and number of features of TcUH, and compared it to other few available bacterial UH structures. Each monomer presents two domains and one NAD+ molecule. Superpositions revealed differences in the relative orientation of domains within...
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Abstract A sample of Apolipoprotein E3 used in the original structure determination by X-ray crystallography (PDB code 1NFN) was crystallized under different conditions and its structure determined by molecular replacement at 298° K. The original model (1NFN) began at amino acid 23 and ended at amino acid 164, but the amino acid segment 81 through 91 (a loop between helices) was not visible in the electron density and presumed disordered. The model reported here is essentially identical to 1NFN,...
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Electronic circular dichroism is one of the most used spectroscopic techniques for peptide and protein structural characterization. However, while valuable experimental spectra exist for α-helix, β-sheet and random coil secondary structures, previous studies showed important discrepancies for β-turns, limiting their use as a reference for structural studies. In this paper, we simulated circular dichroism spectra for the best-characterized β-turns in peptides, namely types I, II, I′ and II′. In p...
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Abstract The β subunit of bovine luteinizing hormone (LH) was crystallized and its structure solved to 3.15 ​A resolution by molecular replacement using human chorionic gonadotropin (hCG) β subunit as search model. The asymmetric unit contains two copies of the β subunit that are related by a non-crystallographic symmetry (NCS) two-fold axis, both copies of which contain proteolytic cleavages after amino acid 100. It is noteworthy that the oligosaccharide moieties covalently attached at asparagi...
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Ribonucleic acid (RNA) molecules are master regulators of cells. They are involved in a variety of molecular processes: they transmit genetic information, sense cellular signals and communicate responses, and even catalyze chemical reactions. As in the case of proteins, RNA function is dictated by its structure and by its ability to adopt different conformations, which in turn is encoded in the sequence. Experimental determination of high-resolution RNA structures is both laborious and difficult...
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Abstract Present work proposes a unique and simple method for synthesizing small and uniform Barium sulfate particles within a novel auxiliary solution based on the glycerol. Glycerol acts as a molecular spacer and prevents growth and agglomeration of the synthesizing particles. Barium sulfate is the isomorph of PbSO4 and provides the centers for nucleation of PbSO4 particles during the discharge process. Smaller particles of BaSO4 leads to more and smaller PbSO4 particles per volume of Negative...
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