Observations on Molecular Weight Determinations on Polyacrylamide Gel

1. A simple internal calibration technique was used to construct accurate molecular weight-mobility profiles on polyacrylamide gels with well characterized proteins, and the existence of a critical point was observed for small polypeptides. The useful size range limits were determined for several gel concentrations. Apparent molecular weights generally fell within 5 to 6% of literature values, although a few anomalous proteins, notably...