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Analysis of the proteomic profile of chronic pressure ulcers
Published on May 1, 2012in Wound Repair and Regeneration 2.95
· DOI :10.1111/j.1524-475X.2012.00791.x
Laura E. Edsberg13
Estimated H-index: 13
(Daemen College),
Jennifer T. Wyffels8
Estimated H-index: 8
(Daemen College)
+ 1 AuthorsKristin M. Fries6
Estimated H-index: 6
(Daemen College)
Abstract
Analysis of the proteomic profile of pressure ulcers over time is a critical step in the identification of biomarkers of healing or nonhealing in pressure ulcers. The wound fluid from 32 subjects with 42 pressure ulcers was evaluated over 6 weeks at 15 time points. Samples specific to both the interior and the periphery of the wound bed were collected. Antibody screening arrays, isobaric tags for relative and absolute quantitation with mass spectrometry and multiplexed microarrays were used to characterize wound fluid and results were correlated with clinical outcome. Twenty-one proteins were found to distinguish between healed and chronic wounds and 19 proteins were differentially expressed between the interior and periphery of wounds. Four proteins, pyruvate kinase isozymes M1/M2, profilin-1, Ig lambda-1 chain C regions, and Ig gamma-1 chain C region, were present in lower levels for periphery samples when compared to interior samples and six proteins, keratin, type II cytoskeletal 6A (KRT6A), keratin, type I cytoskeletal 14, S100 calcium binding proteins A7, alpha-1-antitrypsin precursor, hemoglobin subunit alpha, and hemoglobin subunit beta, were present in higher levels in periphery samples when compared with interior samples. S100 calcium binding protein A6, S100 calcium binding protein A7, and soluble receptor for advanced glycation end-products had higher levels in the periphery of chronic wounds vs. the interior in planar arrays. A significant temporal trend was noted for monokine induced by gamma interferon (MIG), synonomous with chemokine (C-X-C motif) ligand 9 (CXCL9), which increased as wounds healed and remained nearly constant for ulcers that were not approaching closure.
  • References (40)
  • Cited By (27)
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References40
Published on Jan 1, 1993
van Rijswijk L1
Estimated H-index: 1
35 Citations
Published on Nov 1, 1993
Frantz R1
Estimated H-index: 1
,
Xakellis Gc1
Estimated H-index: 1
,
Arteaga M1
Estimated H-index: 1
23 Citations
Published on Jan 1, 2007in Sub-cellular biochemistry
Claus W. Heizmann72
Estimated H-index: 72
(University of Zurich),
G.E. Ackermann1
Estimated H-index: 1
(University of Zurich),
A. Galichet1
Estimated H-index: 1
(University of Zurich)
The S100 proteins are exclusively expressed in vertebrates and are the largest subgroup within the superfamily of EF-hand Ca 2+ -binding proteins
117 Citations Source Cite
Published on Mar 1, 2011in Wound Repair and Regeneration 2.95
Gregory S. Schultz56
Estimated H-index: 56
(University of Florida),
Jeffrey M. Davidson56
Estimated H-index: 56
(Vanderbilt University Medical Center)
+ 2 AuthorsIra M. Herman41
Estimated H-index: 41
(Tufts University)
Here, we define dynamic reciprocity (DR) as an ongoing, bidirectional interaction amongst cells and their surrounding microenvironment. In the review, we posit that DR is especially meaningful during wound healing as the DR-driven biochemical, biophysical and cellular responses to injury play pivotal roles in regulating tissue regenerative responses. Such cell-extracellular matrix interactions not only guide and regulate cellular morphology, but cellular differentiation, migration, proliferation...
215 Citations Source Cite
Published on Mar 15, 2003in Journal of Immunology 4.54
Carle Ryckman8
Estimated H-index: 8
(Laval University),
Karen Vandal10
Estimated H-index: 10
(Laval University)
+ 2 AuthorsPhilippe A. Tessier31
Estimated H-index: 31
(Laval University)
S100A8 and S100A9 are small calcium-binding proteins that are highly expressed in neutrophil and monocyte cytosol and are found at high levels in the extracellular milieu during inflammatory conditions. Although reports have proposed a proinflammatory role for these proteins, their extracellular activity remains controversial. In this study, we report that S100A8, S100A9, and S100A8/A9 caused neutrophil chemotaxis at concentrations of 10−12–10−9 M. S100A8, S100A9, and S100A8/A9 stimulated sheddi...
499 Citations Source Cite
Published on Apr 1, 1997in Journal of Investigative Dermatology 6.45
Matthias Goebeler47
Estimated H-index: 47
(University of Würzburg),
Teizo Yoshimura34
Estimated H-index: 34
+ 3 AuthorsReinhard Gillitzer24
Estimated H-index: 24
(University of Würzburg)
Activation of endothelium is a critical event during the initiation of inflammatory processes and is associated with the induction of cell adhesion molecules and cytokines. The latter include chemotactically active cytokines (chemokines) that promote leukocyte diapedesis from the circulation to sites of evolving inflammation. In this study we evaluated the chemokine repertoire of human endotheial cells derived from the skin (HDMFCs) and regulation of these chemokines by cytokines. HDMECs and an ...
101 Citations Source Cite
Published on Oct 1, 2000in Current Opinion in Chemical Biology 7.57
Steven P. Gygi137
Estimated H-index: 137
(Harvard University),
Ruedi Aebersold145
Estimated H-index: 145
Abstract Proteomics is the systematic analysis of the proteins expressed by a cell or tissue, and mass spectrometry is its essential analytical tool. In the past two years, incremental advances in standard proteome technology have increased the speed of protein identification with higher levels of automation and sensitivity. Furthermore, new approaches have provided landmark advances in determining functionally relevant properties of proteins, including their quantity and involvement within prot...
249 Citations Source Cite
Published on Jul 1, 2004in Journal of Investigative Dermatology 6.45
Richard L. Eckert37
Estimated H-index: 37
,
Ann-Marie Broome20
Estimated H-index: 20
(Case Western Reserve University)
+ 3 AuthorsKathleen C. Lee3
Estimated H-index: 3
(Case Western Reserve University)
The S100 proteins comprise a family of 21 low molecular weight (9–13 kDa) proteins that are characterized by the presence of two calcium-binding EF-hand motifs. Fourteen S100 protein genes are located within the epidermal differentiation complex on human chromosome 1q21 and 13 S100 proteins (S100A2, S100A3, S100A4, S100A6, S100A7, S100A8, S100A9, S100A10, S100A11, S100A12, S100A15, S100B, and S100P) are expressed in normal and/or diseased epidermis. S100 proteins exist in cells as anti-parallel ...
319 Citations Source Cite
Published on Dec 1, 2009in Analytical Chemistry 6.04
Vivek N. Bhatia3
Estimated H-index: 3
,
David H. Perlman22
Estimated H-index: 22
+ 1 AuthorsMark E. McComb23
Estimated H-index: 23
(Boston University)
In order that biological meaning may be derived and testable hypotheses may be built from proteomics experiments, assignments of proteins identified by mass spectrometry or other techniques must be supplemented with additional notation, such as information on known protein functions, protein−protein interactions, or biological pathway associations. Collecting, organizing, and interpreting this data often requires the input of experts in the biological field of study, in addition to the time-cons...
164 Citations Source Cite
Published on May 18, 2010in International Wound Journal 2.38
Jennifer T. Wyffels8
Estimated H-index: 8
(Daemen College),
Kristin M. Fries6
Estimated H-index: 6
(Daemen College)
+ 5 AuthorsLaura E. Edsberg13
Estimated H-index: 13
(Daemen College)
The incidence rate of pressure ulcers in the USA ranges from 0·4% to 38% in acute care settings and from 2·2% to 23·9% in long-term care settings, and their treatment costs are in the billions of dollars yearly. The proteome of wound fluid may contain early indicators or biomarkers associated with healing in pressure ulcers that would enable treatment regimes to be optimised for each individual. Wound fluid was collected from the interior and periphery of 19 chronic pressure ulcers at 15 time po...
13 Citations Source Cite
Cited By27
Published on Aug 1, 2015in Rechtsmedizin 0.64
M. Fattouh , C.T. Buschmann7
Estimated H-index: 7
(Charité)
+ 3 AuthorsMichael Tsokos30
Estimated H-index: 30
(Charité)
Hintergrund Die Expression leukozytarer und endothelialer Adhasionsmolekule aus der Gruppe der Selektin-, Integrin- und der Immunglobulinsuperfamilie in primar heilenden Hautwunden ist tierexperimentell sowie an humanem Operations- und Autopsiematerial untersucht worden. Fur die Dekubituswunde existieren bezuglich der Gewebeexpression leukozytarer und endothelialer Adhasionsmolekule bisher allerdings keine gesicherten Erkenntnisse.
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Published on Jan 1, 2014
11 CHAPTER 1-INTRODUCTION 13 EPIDEMIOLOGY OF ASTHMA 13 PATHOGENESIS OF ASTHMA 13 BIOMARKERS DEFINITIONS 16 VALUE OF BIOMARKERS 16 BEYOND BIOMARKERS THEMSELVES 18 IN VIVO AND IN VITRO STUDIES 18 PROTEOMICS 19 BIOMARKERS AND ASTHMA 23 HYPOTHESES 24 CHAPTER 2MATERIALS AND METHODS 26 BIRTH COHORT SUBGROUPS AND SAMPLES 26 ASTHMA BIOMARKER DISCOVERY (PARENT PROJECT) 27 ANTIBODY ARRAY STUDIES 27 ELISA QUANTITATION OF CANDIDATE PROTEINS 29 IN VIVO STUDIES OF EPO’S BIOLOGICAL EFFECTS 31 Bronchoconstricti...
Markus W. Löffler8
Estimated H-index: 8
(University of Tübingen),
Heiko Schuster12
Estimated H-index: 12
(University of Tübingen)
+ 1 AuthorsStefan Beckert16
Estimated H-index: 16
(University of Tübingen)
Valid and reproducible sampling techniques as well as processing protocols are required for the assessment of biomarkers and mediators contained in wound exudate. Moreover, the ideal technique should be easy to use even in daily clinical routine. This is challenging since wound fluid represents an inhomogeneous mixture of different exogenous and endogenous sources. Analyzing wound fluid, however, may facilitate clinical decision making. Many techniques for obtaining wound fluid have been describ...
8 Citations Source Cite
Published on Jul 1, 2015in Journal of Spinal Cord Medicine 1.88
Laura E. Edsberg13
Estimated H-index: 13
(Daemen College),
Jennifer T. Wyffels8
Estimated H-index: 8
(Daemen College)
+ 2 AuthorsPamela Houghton4
Estimated H-index: 4
(University of Western Ontario)
AbstractObjectiveTo determine whether the biochemistry of chronic pressure ulcers differs between patients with and without chronic spinal cord injury (SCI) through measurement and comparison of the concentration of wound fluid inflammatory mediators, growth factors, cytokines, acute phase proteins, and proteases.DesignSurvey.SettingTertiary spinal cord rehabilitation center and skilled nursing facilities.ParticipantsTwenty-nine subjects with SCI and nine subjects without SCI (>18 years) with at...
4 Citations Source Cite
Published on Nov 1, 2013in Advances in wound care
Tomasz Arodz3
Estimated H-index: 3
,
Danail Bonchev15
Estimated H-index: 15
,
Robert F. Diegelmann45
Estimated H-index: 45
Objective: The wound healing process is well-understood on the cellular and tissue level; however, its complex molecular mechanisms are not yet uncovered in their entirety. Viewing wounds as perturbed molecular networks provides the tools for analyzing and optimizing the healing process. It helps to answer specific questions that lead to better understanding of the complexity of the process. What are the molecular pathways involved in wound healing? How do these pathways interact with each other...
10 Citations Source Cite
Published on Jan 1, 2015in Matrix Biology 8.14
Arti V. Shinde6
Estimated H-index: 6
(Albany Medical College),
Rhiannon M. Kelsh4
Estimated H-index: 4
(Albany Medical College)
+ 3 AuthorsPaula J. McKeown-Longo21
Estimated H-index: 21
(Albany Medical College)
Abstract Prompt deposition of fibronectin-rich extracellular matrix is a critical feature of normal development and the host-response to injury. Fibronectin isoforms that include the EDA and EDB domains are prominent in these fibronectin matrices. We now report using human dermal fibroblast cultures that the EDA domain of fibronectin or EDA-derived peptides modeled after the C–C′ loop promote stress fiber formation and myosin-light chain phosphorylation. These changes are accompanied by an incre...
18 Citations Source Cite
Laura Mayol16
Estimated H-index: 16
,
Daniela De Stefano15
Estimated H-index: 15
+ 9 AuthorsAngela Gallo1
Estimated H-index: 1
In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical–chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoela...
18 Citations Source Cite
Published on Jan 1, 2013in Ulcers
Hannah Trøstrup5
Estimated H-index: 5
,
Thomas Bjarnsholt49
Estimated H-index: 49
+ 2 AuthorsClaus Moser36
Estimated H-index: 36
Chronic wounds are a growing socioeconomic problem in the western world. Knowledge on recalcitrant wounds relies on in vitro studies or clinical observations, and there is emerging evidence on the clinical impact of bacterial biofilm on skin healing. Chronic wounds are locked in the inflammatory state of wound healing, and there are multiple explanations for this arrest with the theory of exaggerated proteolysis as the most commonly accepted. Previously, there has not been enough focus on the di...
14 Citations Source Cite
Published on Jan 1, 2013in Journal of Translational Medicine 4.20
Brett A. Chromy23
Estimated H-index: 23
(University of California, Davis),
Angela Eldridge7
Estimated H-index: 7
(University of California, Davis)
+ 6 AuthorsPaul A. Luciw45
Estimated H-index: 45
(University of California, Davis)
Background: The ability to forecast whether a wound will heal after closure without further debridement(s), would provide substantial benefits to patients with severe extremity trauma. Methods: Wound effluent is a readily available material which can be collected without disturbing healthy tissue. For analysis of potential host response biomarkers, forty four serial combat wound effluent samples from 19 patients with either healing or failing traumatic- and other combat-related wounds were exami...
13 Citations Source Cite
View next paperDifferential proteomic analysis distinguishes tissue repair biomarker signatures in wound exudates obtained from normal healing and chronic wounds.