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Analysis of the proteomic profile of chronic pressure ulcers

Published on May 1, 2012in Wound Repair and Regeneration 2.95
· DOI :10.1111/j.1524-475X.2012.00791.x
Laura E. Edsberg13
Estimated H-index: 13
(Daemen College),
Jennifer T. Wyffels8
Estimated H-index: 8
(Daemen College)
+ 1 AuthorsKristin M. Fries6
Estimated H-index: 6
(Daemen College)
Abstract
Analysis of the proteomic profile of pressure ulcers over time is a critical step in the identification of biomarkers of healing or nonhealing in pressure ulcers. The wound fluid from 32 subjects with 42 pressure ulcers was evaluated over 6 weeks at 15 time points. Samples specific to both the interior and the periphery of the wound bed were collected. Antibody screening arrays, isobaric tags for relative and absolute quantitation with mass spectrometry and multiplexed microarrays were used to characterize wound fluid and results were correlated with clinical outcome. Twenty-one proteins were found to distinguish between healed and chronic wounds and 19 proteins were differentially expressed between the interior and periphery of wounds. Four proteins, pyruvate kinase isozymes M1/M2, profilin-1, Ig lambda-1 chain C regions, and Ig gamma-1 chain C region, were present in lower levels for periphery samples when compared to interior samples and six proteins, keratin, type II cytoskeletal 6A (KRT6A), keratin, type I cytoskeletal 14, S100 calcium binding proteins A7, alpha-1-antitrypsin precursor, hemoglobin subunit alpha, and hemoglobin subunit beta, were present in higher levels in periphery samples when compared with interior samples. S100 calcium binding protein A6, S100 calcium binding protein A7, and soluble receptor for advanced glycation end-products had higher levels in the periphery of chronic wounds vs. the interior in planar arrays. A significant temporal trend was noted for monokine induced by gamma interferon (MIG), synonomous with chemokine (C-X-C motif) ligand 9 (CXCL9), which increased as wounds healed and remained nearly constant for ulcers that were not approaching closure.
  • References (40)
  • Citations (27)
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References40
Newest
Published on Oct 1, 2011in Advances in Skin & Wound Care 1.38
Jennifer T. Wyffels8
Estimated H-index: 8
,
Laura E. Edsberg13
Estimated H-index: 13
OBJECTIVE: To describe the temporal relationship between the quantity of granulation tissue in a chronic pressure ulcer (PrU) and its clinical outcome. DESIGN: Study participants were seen on days 0, 1, 2, 3, 4, 7, 8, 9, 10, 11, 14, 21, 28, 35, and 42. On each visit, the wounds were digitally photographed with a 3-cm2 calibration target. Images were analyzed using VeV MD (version 1.1.14; VERG Inc, Winnipeg, Manitoba, Canada) and Adobe Photoshop CS3 Extended (version 10.0.1; Adobe Systems Inc, Sa...
8 Citations Source Cite
Published on Mar 1, 2011in Wound Repair and Regeneration 2.95
Gregory S. Schultz61
Estimated H-index: 61
(University of Florida),
Jeffrey M. Davidson57
Estimated H-index: 57
(Vanderbilt University Medical Center)
+ 2 AuthorsIra M. Herman43
Estimated H-index: 43
(Tufts University)
Here, we define dynamic reciprocity (DR) as an ongoing, bidirectional interaction amongst cells and their surrounding microenvironment. In the review, we posit that DR is especially meaningful during wound healing as the DR-driven biochemical, biophysical and cellular responses to injury play pivotal roles in regulating tissue regenerative responses. Such cell-extracellular matrix interactions not only guide and regulate cellular morphology, but cellular differentiation, migration, proliferation...
219 Citations Source Cite
Published on Sep 3, 2010in Journal of Proteome Research 3.95
Sabine A. Eming36
Estimated H-index: 36
,
Manuel Koch42
Estimated H-index: 42
+ 6 AuthorsJay W. Fox52
Estimated H-index: 52
Chronic wounds associated with vascular disease, diabetes mellitus, or aging are leading causes of morbidity in western countries and represent an unresolved clinical problem. The development of innovative strategies to promote tissue repair is therefore an important task that requires a more thorough analysis of the underlying molecular pathophysiology. We propose that the understanding of the complex biological events that control tissue repair or its failure largely benefits from a broad anal...
114 Citations Source Cite
Published on Sep 1, 2010in Nutrition 3.73
Shinji Iizaka16
Estimated H-index: 16
(University of Tokyo),
Hiromi Sanada28
Estimated H-index: 28
(University of Tokyo)
+ 4 AuthorsJunko Sugama22
Estimated H-index: 22
(Kanazawa University)
Abstract Objective Protein loss from wound fluid is usually recognized as one of the factors contributing to the deterioration of the nutritional status in older patients with severe pressure ulcers. We quantified the protein loss owing to pressure ulcers and investigated associations with wound-related factors and nutritional status. Methods This cross-sectional study included 25 patients (≥60 y) from 10 institutions, with full-thickness pressure ulcers. Wound fluid was collected once after acc...
18 Citations Source Cite
Published on May 18, 2010in International Wound Journal 2.38
Jennifer T. Wyffels8
Estimated H-index: 8
(Daemen College),
Kristin M. Fries6
Estimated H-index: 6
(Daemen College)
+ 5 AuthorsLaura E. Edsberg13
Estimated H-index: 13
(Daemen College)
The incidence rate of pressure ulcers in the USA ranges from 0·4% to 38% in acute care settings and from 2·2% to 23·9% in long-term care settings, and their treatment costs are in the billions of dollars yearly. The proteome of wound fluid may contain early indicators or biomarkers associated with healing in pressure ulcers that would enable treatment regimes to be optimised for each individual. Wound fluid was collected from the interior and periphery of 19 chronic pressure ulcers at 15 time po...
13 Citations Source Cite
Published on Jan 1, 2010in Wound Repair and Regeneration 2.95
Shinji Iizaka16
Estimated H-index: 16
(University of Tokyo),
Hiromi Sanada28
Estimated H-index: 28
(University of Tokyo)
+ 6 AuthorsJunko Sugama22
Estimated H-index: 22
(Kanazawa University)
Evaluation of wound fluid characteristics for pressure ulcer (PU) assessment in clinical settings remains subjective, requiring considerable expertise. This cross-sectional study focused on nutritional markers in wound fluid as possible objective tools and investigated whether they reflect the PU status according to the healing phase, infection, and granulation, especially after adjusting for serum values. Twenty-eight patients with 32 full-thickness PUs were studied. The concentration of albumi...
20 Citations Source Cite
Published on Dec 1, 2009in Analytical Chemistry 6.04
Vivek N. Bhatia3
Estimated H-index: 3
,
David H. Perlman23
Estimated H-index: 23
+ 1 AuthorsMark E. McComb27
Estimated H-index: 27
(Boston University)
In order that biological meaning may be derived and testable hypotheses may be built from proteomics experiments, assignments of proteins identified by mass spectrometry or other techniques must be supplemented with additional notation, such as information on known protein functions, protein−protein interactions, or biological pathway associations. Collecting, organizing, and interpreting this data often requires the input of experts in the biological field of study, in addition to the time-cons...
155 Citations Source Cite
Kenneth Hsu17
Estimated H-index: 17
(University of New South Wales),
Chantrakorn Champaiboon2
Estimated H-index: 2
(University of Minnesota)
+ 5 AuthorsMark C. Herzberg35
Estimated H-index: 35
The calgranulins are a subgroup of proteins in the S100 family (calgranulin A, S100A8; calgranulin B, S100A9 and calgranulin C, S100A12) that provide protective anti-infective and anti-inflammatory functions for the mammalian host. In this review, we discuss the structure-function relationships whereby S100A8 and S100A9, and for comparison, S100A12, provide intra- and extracellular protection during the complex interplay between infection and inflammation and how the calgranulins are regulated t...
103 Citations Source Cite
Published on Oct 1, 2009in British Journal of Cancer 5.92
Anna Birgersdotter6
Estimated H-index: 6
,
Karl R. N. Baumforth13
Estimated H-index: 13
+ 5 AuthorsIngemar Ernberg50
Estimated H-index: 50
Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma
15 Citations Source Cite
Published on Sep 1, 2008in American Journal of Pathology 4.07
Cecelia C. Yates16
Estimated H-index: 16
(Tuskegee University),
Diana Whaley8
Estimated H-index: 8
(University of Pittsburgh)
+ 3 AuthorsAlan Wells63
Estimated H-index: 63
(Tuskegee University)
In skin wounds, the chemokine CXCR3 receptor appears to play a key role in coordinating the switch from regeneration of the ontogenically distinct mesenchymal and epithelial compartments toward maturation. However, because CXCR3 equivalently binds four different ELR-devoid CXC chemokines (ie, PF4/CXCL4, IP-10/CXCL10, MIG/CXCL9, and IP-9/CXCL11), we sought to identify the ligand that coordinates epidermal coverage with the maturation of the underlying superficial dermis. Because CXCL11 (IP-9 or I...
39 Citations Source Cite
Cited By27
Newest
Published on Feb 1, 2019in Journal of Proteomics 3.72
Nayara Rodrigues Vieira Cavassan1
Estimated H-index: 1
(Sao Paulo State University),
Caio Cavassan de Camargo3
Estimated H-index: 3
(Sao Paulo State University)
+ 5 AuthorsLucilene Delazari dos Santos16
Estimated H-index: 16
(Sao Paulo State University)
Abstract Chronic venous ulcers affect the quality of life of patients around the world. The aims of this study were to identify the proteins expressed in chronic venous ulcer exudates, to categorize them according to their roles and to correlate them with the clinical and epidemiological aspects of the disease. The study population consisted of 37 ulcers from 28 patients, and the inflammatory exudates of these thirty-seven ulcers were subjected to tryptic digestion and mass spectrometry analysis...
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Published on Dec 2, 2018in Expert Review of Proteomics 3.49
Cristina Herrera6
Estimated H-index: 6
(University of Costa Rica),
Teresa Escalante26
Estimated H-index: 26
(University of Costa Rica)
+ 2 AuthorsJosé María Gutiérrez71
Estimated H-index: 71
(University of Costa Rica)
ABSTRACTIntroduction: Metalloproteinases play key roles in health and disease, by generating novel proteoforms with variable structure and function.Areas covered: This review focuses on the role of endogenous [a Disintegrin and Metalloproteinase (ADAMs), ADAMs with thrombospondin motifs (ADAMTS), and matrix metalloproteinases (MMPs)] and exogenous metalloproteinases in various disease conditions, and describes the application of mass spectrometry-based proteomics to detect qualitative and quanti...
1 Citations Source Cite
Published on Jun 1, 2018in Genomics, Proteomics & Bioinformatics
Erin L. Crowgey2
Estimated H-index: 2
(Alfred I. duPont Hospital for Children),
Jennifer T. Wyffels2
Estimated H-index: 2
(University of Delaware)
+ 2 AuthorsLaura E. Edsberg13
Estimated H-index: 13
(Daemen College)
Abstract Heterotopic ossification (HO) refers to the abnormal formation of bone in soft tissue. Although some of the underlying processes of HO have been described, there are currently no clinical tests using validated biomarkers for predicting HO formation. As such, the diagnosis is made radiographically after HO has formed. To identify potential and novel biomarkers for HO, we used isobaric tags for relative and absolute quantitation (iTRAQ) and high-throughput antibody arrays to produce a sem...
1 Citations Source Cite
Published on Dec 1, 2017in Journal of Orthopaedic Surgery and Research 1.61
Laura E. Edsberg13
Estimated H-index: 13
(Daemen College),
Erin L. Crowgey2
Estimated H-index: 2
(Alfred I. duPont Hospital for Children)
+ 1 AuthorsJennifer T. Wyffels2
Estimated H-index: 2
(University of Delaware)
Background Heterotopic ossification (HO) is a significant problem for wounded warriors surviving high-energy blast injuries; however, currently, there is no biomarker panel capable of globally characterizing, diagnosing, and monitoring HO progression. The aim of this study was to identify biomarkers for HO using proteomic techniques and blood serum.
2 Citations Source Cite
Published on Nov 1, 2017in Journal of Biomaterials Applications 2.08
Krystal R. Fontenot6
Estimated H-index: 6
,
J. Vincent Edwards11
Estimated H-index: 11
+ 5 AuthorsDorne R. Yager24
Estimated H-index: 24
Interfacing nanocellulosic-based biosensors with chronic wound dressings for protease point of care diagnostics combines functional material properties of high specific surface area, appropriate surface charge, and hydrophilicity with biocompatibility to the wound environment. Combining a protease sensor with a dressing is consistent with the concept of an intelligent dressing, which has been a goal of wound-dressing design for more than a quarter century. We present here biosensors with a nanoc...
3 Citations Source Cite
Published on Aug 1, 2017in Clinica Chimica Acta 2.93
Maria Ilies2
Estimated H-index: 2
,
Cristina Iuga6
Estimated H-index: 6
+ 4 AuthorsElke Hammer30
Estimated H-index: 30
Abstract Pre-analytical factors have a significant impact on the integrity of blood samples used for qualitative and quantitative protein profiling. Important factors are the type of the blood collection tube and the anticoagulant used. Only a few studies have been performed to assess these variables by comparing only serum and EDTA plasma collection tubes with some target proteins or peptides. In this study, we investigated the protein profile of blood samples collected in serum, EDTA-, heparin...
4 Citations Source Cite
Published on Jul 2, 2017in Journal of Wound Care 1.67
G. Power1
Estimated H-index: 1
,
Zena Moore19
Estimated H-index: 19
,
Tom O'Connor3
Estimated H-index: 3
Objective: To assess the potential of measurements of pH, exudate composition and temperature in wounds to predict healing outcomes and to identify the methods that are employed to measure them. Method: A systematic review based on the outcomes of a search strategy of quantitative primary research published in the English language was conducted. Inclusion criteria limited studies to those involving in vivo and human participants with an existing or intentionally provoked wound, defined as ‘a bre...
10 Citations Source Cite
Published on Jan 1, 2017
Q. Zeng1
Estimated H-index: 1
(State University of New York System),
L.K. Macri1
Estimated H-index: 1
(State University of New York System)
+ 10 AuthorsDario Stupar2
Estimated H-index: 2
The integration of healing, cell biology, and skin tissue engineering research has been ongoing for nearly one century. In this chapter, we provide a bird’s eye view of skin anatomy and functions, wound healing processes, the challenges and solutions to wound healing. Many techniques and biomaterials have been examined for their potential utility as skin substitutes. Notwithstanding evidence that some strategies have been more successful than others, the ideal skin substitute does not exist. Exi...
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Published on Sep 1, 2016in Advanced Healthcare Materials 5.61
Subhamoy Das7
Estimated H-index: 7
(University of Texas at Austin),
Gunjan Singh3
Estimated H-index: 3
(University of Texas at Austin)
+ 6 AuthorsAaron B. Baker20
Estimated H-index: 20
Abstract Chronic wounds represent a major healthcare and economic problem worldwide. Advanced wound dressings that incorporate bioactive compounds have great potential for improving outcomes in patients with chronic wounds but significant challenges in designing treatments that are effective in long-standing, nonhealing wounds. Here, an optimized wound healing gel was developed that delivers syndecan-4 proteoliposomes ("syndesomes") with fibroblast growth factor-2 (FGF-2) to enhance diabetic wou...
9 Citations Source Cite