Match!

Apoptosis-Inducing Activity of Marine Sponge Haliclona sp. Extracts Collected from Kosrae in Nonsmall Cell Lung Cancer A549 Cells.

Published on Jan 1, 2015in Evidence-based Complementary and Alternative Medicine1.984
路 DOI :10.1155/2015/717959
Woori Bae2
Estimated H-index: 2
,
Hyun Kyung Lim4
Estimated H-index: 4
+ 5 AuthorsJoohee Jung9
Estimated H-index: 9
(Duksung Women's University)
Abstract
Although various anticancer drugs have been developed for the treatment of nonsmall cell lung cancer, chemotherapeutic efficacy is still limited. Natural products such as phytochemicals have been screened as novel alternative materials, but alternative funds such as marine bioresources remain largely untapped. Of these resources, marine sponges have undergone the most scrutiny for their biological activities, including antiinflammatory, antiviral, and anticancer properties. However, the biological mechanisms of the activities of these marine sponges are still unclear. We investigated the anticancer activity of marine sponges collected from Kosrae in Micronesia and examined their mechanisms of action using nonsmall cell lung cancer A549 cells as a model system. Of 20 specimens, the Haliclona sp. (KO1304-328) showed both dose- and time-dependent cytotoxicity. Further, methanol extracts of Haliclona sp. significantly inhibited cell proliferation and cell viability. A549 cells treated with Haliclona sp. demonstrated induced expression of c-Jun N-terminal kinase (JNK), p53, p21, caspase-8, and caspase-3. The percentage of apoptotic cells significantly increased in A549 cultures treated with Haliclona sp. These results indicate that Haliclona sp. induces apoptosis via the JNK-p53 pathway and caspase-8, suggesting that this marine sponge is a good resource for the development of drugs for treatment of nonsmall cell lung cancer.
  • References (23)
  • Citations (7)
馃摉 Papers frequently viewed together
11 Citations
11 Citations
10 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References23
Newest
#1Malte KriegsH-Index: 15
#2Kristin GurtnerH-Index: 9
Last. Ekkehard DikomeyH-Index: 32
view all 16 authors...
Abstract Purpose How EGF receptor (EGFR) inhibition induces cellular radiosensitization and with that increase in tumor control is still a matter of discussion. Since EGFR predominantly regulates cell cycle and proliferation, we studied whether a G1-arrest caused by EGFR inhibition may contribute to these effects. Materials and methods We analyzed human non-small cell lung cancer (NSCLC) cell lines either wild type (wt) or mutated in p53 (A549, H460, vs. H1299, H3122) and HCT116 cells (p21 wt an...
23 CitationsSource
#1A. Hoppers (GSU: Georgia State University)H-Index: 1
#2J. Stoudenmire (GSU: Georgia State University)H-Index: 1
Last. Nicole B. Lopanik (GSU: Georgia State University)H-Index: 10
view all 4 authors...
Aims Sessile marine invertebrates engage in a diverse array of beneficial interactions with bacterial symbionts. One feature of some of these relationships is the presence of bioactive natural products that can defend the holobiont from predation, competition or disease. In this study, we investigated the antimicrobial activity and microbial community of a common temperate sponge from coastal North Carolina. Methods and Results The sponge was identified as a member of the genus Haliclona, a prol...
7 CitationsSource
#1Cassiana Macagnan Viau (Amazon.com)H-Index: 1
Last. Jenifer Saffi (Amazon.com)H-Index: 28
view all 4 authors...
Background The 3尾, 6尾, 16尾-trihydroxylup-20(29)-ene (TTHL) is a pentacyclic triterpene obtained from the medicinal plant Combretum leprosum Mart. In folk medicine, this plant is popularly known as mofumbo, cipoaba or mufumbo, and is used to treat several diseases associated with inflammation and pain.
16 CitationsSource
Formononetin is a novel herbal isoflavonoid isolated from Astragalus membranaceus and possesses antitumorigenic properties. In the present study, we investigated the anti-proliferative effects of formononetin on human non-small cell lung cancer (NSCLC), and further elucidated the molecular mechanism underlying the anti-tumor property. MTT assay showed that formononetin treatment significantly inhibited the proliferation of two NSCLC cell lines including A549 and NCI-H23 in a time- and dose-depen...
19 Citations
#1M. NazemiH-Index: 1
#2M. Alidoust Salimi (IAU: Islamic Azad University)H-Index: 1
Last. O. AhmadzadehH-Index: 1
view all 6 authors...
Summary In this study, antifungal and antibacterial activities of diethyl ether, methanol and aqueous extracts of Haliclona sp. were assessed (in vitro). The antibacterial activity of the extracts was determined by broth dilution methods against clinical Gram-negative bacteria: Escherichia coli , Pseudomonas aeruginosa and Gram-positive bacteria: Staphylococcus aureus aureus , Bacillus subtilis spizizenii . The antifungal activity of the extracts was determined by using a broth microdilution tes...
4 CitationsSource
#1Hyun Kyung LimH-Index: 4
#2Woori BaeH-Index: 2
Last. Joohee Jung (Duksung Women's University)H-Index: 9
view all 4 authors...
Drug development using marine bioresources is limited even though the ocean occupies about 70% of the earth and contains a large number of biological materials. From the screening test of the marine sponge extracts, we found Hyrtios sp. sponge collected from Chuuk island, Micronesia. In this study, the Hyrtios sp. extract was examined for anticancer activity against human colorectal carcinoma RKO cells that are wildtype for p53 and RKO-E6 that are p53 defective. The Hyrtios sp. extract dose-depe...
11 CitationsSource
#1Kalimuthu Senthilkumar (Pukyong National University)H-Index: 16
#2Jayachandran Venkatesan (Pukyong National University)H-Index: 29
Last. Se-Kwon Kim (Pukyong National University)H-Index: 85
view all 4 authors...
Abstract The term 鈥渁ngiogenic switch鈥 refers to a time-restricted event during tumor progression where the balance between pro- and anti-angiogenic factors, resulting in the transition from dormant avascularized hyperplasia to outgrowing vascularized tumor and eventually to malignant tumor progression. Targeting angiogenesis and its mechanistic pathways are critical target for cancer therapy. Recently, marine derived compounds, plays major role in cancer research. Several sponge derived compound...
13 CitationsSource
#1Eduardo Reina (National University of Colombia)H-Index: 2
#2Freddy Alejandro Ramos Rodr铆guez (National University of Colombia)H-Index: 11
Last. Luis Fernando Ospina (National University of Colombia)H-Index: 7
view all 5 authors...
Objectives This study aims to evaluate the effect of prostaglandins isolated from soft coral Plexaura homomalla, collected in Colombian Caribbean Sea, on in vivo and in vitro inflammation models. Methods Extracts from P.鈥塰omomalla were fractionated and sequentially chromatographed to obtain the prostaglandins: (15R)-PGA2 (1), (15R)-PGA2-Me (2), (15R)-O-Ac-PGA2 (3), (15R)-O-Ac-PGA2-Me (4) and (15R)-PGE2 (5) in addition to three semi-synthetic prostaglandins obtained by transformations of the natu...
7 CitationsSource
#1Wen-Chi Wei (AS: Academia Sinica)H-Index: 10
#2Ping-Jyun SungH-Index: 28
Last. Ning-Sun Yang (AS: Academia Sinica)H-Index: 24
view all 6 authors...
This review reports details on the natural products isolated from Taiwan soft corals during the period 2008鈥2012 focusing on their in vitro and/or in vivo anti-inflammatory activities. Chemical structures, names, and literature references are also reported. This review provides useful and specific information on potent anti-inflammatory marine metabolites for future development of immune-modulatory therapeutics.
50 CitationsSource
#1Hiroyuki Yamazaki (Tohoku Pharmaceutical University)H-Index: 12
#2Defny S. Wewengkang (Tohoku Pharmaceutical University)H-Index: 6
Last. Michio Namikoshi (Tohoku Pharmaceutical University)H-Index: 33
view all 7 authors...
The extract of an Indonesian marine sponge Haliclona sp. showed potent cytotoxicity against human solid cancer cell lines, MCF-7 (breast), LNCap (prostate), Caco-2 (colon) and HCT-15 (colon) cells. Study on nuclear morphological changes and flow cytometric analysis suggested that the component(s) in the extract would induce an apoptosis to these cancer cells. Bioassay-guided isolation yielded two pentacyclic alkaloids, papuamine (1) and haliclonadiamine (2), which inhibited cell proliferation of...
14 CitationsSource
Cited By7
Newest
#1Woro Anindito Sri Tunjung (UGM: Gadjah Mada University)H-Index: 1
#2Puspa Restu Sayekti (UGM: Gadjah Mada University)
Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T4...
Source
#1Sweta AgrawalH-Index: 1
#2Sachin ChauguleH-Index: 1
Last. Madhavi IndapH-Index: 1
view all 5 authors...
Background The marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. The present study was focused on evaluating the anti-angiogenic and anti- proliferative activities of methanolic extract of Euchelus asper (EAME).
1 CitationsSource
#1Hyun Kyung Lim (Duksung Women's University)H-Index: 4
#2Kyoung Mee Kim (Duksung Women's University)H-Index: 3
Last. Joohee Jung (Duksung Women's University)H-Index: 9
view all 5 authors...
Docetaxel (DTX) is an effective commercial anticancer agent for chemotherapy in non鈥搒mall cell lung cancer (NSCLC), breast cancer, gastric cancer, and prostate cancer, but its adverse effects including edema, neurotoxicity, and hair loss limit its application. To improve the chemotherapeutic efficacy of DTX and reduce adverse effects, combination therapy is one of the alternative methods. So chrysin, which has various biological activities including anticancer effects, was considered. In vitro, ...
4 CitationsSource
#1Saiman ZhangH-Index: 1
#2Huilong OuH-Index: 1
Last. Ming ChenH-Index: 5
view all 7 authors...
We report the development of sponge Haliclona sp. spicules, referred to as SHS, and its topical application in skin delivery of hydrophilic biomacromolecules, a series of fluorescein isothiocyanate-dextrans (FDs). SHS are silicious oxeas which are sharp-edged and rod-shaped (鈭120 渭m in length and 鈭7 渭m in diameter). SHS can physically disrupt skin in a dose-dependent manner and retain within the skin over at least 72 h, which allows sustained skin penetration of hydrophilic biomacromolecules. Th...
2 CitationsSource
#1Feifei WuH-Index: 2
#2Li Zhou (ZJNU: Zhejiang Chinese Medical University)H-Index: 3
Last. Thomas Efferth (University of Mainz)H-Index: 67
view all 13 authors...
With the highest cancer incidence rate, lung cancer, especially non-small cell lung cancer (NSCLC), is the leading cause of cancer death in the world. Tea (leaves of Camellia sinensis) has been widely used as a traditional beverage beneficial to human health, including anti-NSCLC activity. Theabrownin (TB) is one major kind of tea pigment responsible for the beneficial effects of tea liquor. However, its effect on NSCLC is unknown. The aim of the present study was to evaluate anti-proliferative ...
9 CitationsSource
#1Zahid Manzoor (Jeju National University)H-Index: 6
#2Irshad Ali (Jeju National University)H-Index: 4
Last. Young-Sang Koh (Jeju National University)H-Index: 18
view all 4 authors...
Marine algae exhibit broad spectrum anti-bacterial and anti-inflammatory activities. Acrosorium polyneurum (A. polyneurum) is a marine red alga and belongs to the family Delesseriaceae. The present research evaluates the anti-inflammatory effects of A. polyneurum extract (APE) on pro-inflammatory cytokine production. APE demonstrated substantial inhibitory effects on production of pro-inflammatory cytokine in bone marrow-derived macrophages (BMDMs). APE pre-treatment in the lipopolysaccharide (L...
Source
Source