Screening of hepatocyte proteins binding to complete S protein of hepatitis B virus by yeast-two hybrid system

To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes.We constructed bait plasmid expressing complete S protein of HBV by cloning the gene of complete S protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (alpha type) containing liver cDNA library plasmid in 2XYPDA...