Genome editing in Ustilago maydis using the CRISPR–Cas system
Abstract
This communication describes the establishment of the type II bacterial CRISPR-Cas9 system to efficiently disrupt target genes in the fungal maize pathogen Ustilago maydis. A single step transformation of a self-replicating plasmid constitutively expressing the U. maydis codon-optimized cas9 gene and a suitable sgRNA under control of the U. maydis U6 snRNA promoter was sufficient to induce genome editing. On average 70% of the progeny of a...
Paper Details
Title
Genome editing in Ustilago maydis using the CRISPR–Cas system
Published Date
Apr 1, 2016
Journal
Volume
89
Pages
3 - 9
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