A rapid and general assay for monitoring endogenous gene modification.

Volume: 649, Pages: 247 - 256
Published: Jan 1, 2010
Abstract
The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of...
Paper Details
Title
A rapid and general assay for monitoring endogenous gene modification.
Published Date
Jan 1, 2010
Volume
649
Pages
247 - 256
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