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Journal of Structural Biology
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3.75
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3985
Papers 3981
1 page of 399 pages (3,981 results)
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#1Fabian EisensteinH-Index: 2
#2Radostin Danev (UTokyo: University of Tokyo)H-Index: 1
Last.Martin PilhoferH-Index: 18
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Abstract The power of cryo-electron tomography (cryoET) lies in its capability to characterize macromolecules in their cellular context. Structure determination by cryoET, however, is time-consuming compared to single particle approaches. A recent study reported significant acceleration of data acquisition by a fast-incremental single-exposure (FISE) tilt series scheme. Here we improved the method and evaluated its efficiency and performance. We show that (1) FISE combined with the latest genera...
1 CitationsSource
#1Kornelia M. Mikula (UH: University of Helsinki)H-Index: 3
#2R. Kolodziejczyk (UH: University of Helsinki)H-Index: 4
Last.Adrian Goldman (University of Leeds)H-Index: 35
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Abstract The gram-negative bacterium Moraxella catarrhalis infects humans exclusively, causing various respiratory tract diseases, including acute otitis media in children, septicaemia or meningitis in adults, and pneumonia in the elderly. To do so, M. catarrhalis expresses virulence factors facilitating its entry and survival in the host. Among them are the ubiquitous surface proteins (Usps): A1, A2, and A2H, which all belong to the trimeric autotransporter adhesin family. They bind extracellul...
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#1Valentina Loconte (UNIPD: University of Padua)H-Index: 2
#2I. Menozzi (University of Parma)H-Index: 2
Last.Rodolfo Berni (University of Parma)H-Index: 26
view all 7 authors...
Abstract The inherent amyloidogenic potential of wild type transthyretin (TTR) is enhanced by a large number of point mutations, which destabilize the TTR tetramer, thereby promoting its disassembly and pathological aggregation responsible for TTR-related amyloidosis. TTR stabilizers are able to interact with the thyroxine-binding sites of TTR, stabilizing its tetrameric native state and inhibiting amyloidogenesis. Herein, we report on in vitro, ex vivo, and X-ray analyses to assess the TTR stru...
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#1Bartosz Marzec (Edin.: University of Edinburgh)H-Index: 7
#2J.M. Walker (Edin.: University of Edinburgh)H-Index: 2
Last.Fabio Nudelman (Edin.: University of Edinburgh)H-Index: 22
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Abstract Coccolithophores are marine phytoplankton that are among the most prolific calcifiers widespread in Earth’s oceans, playing a crucial role in the carbon cycle and in the transport of organic matter to the deep sea. These organisms produce highly complex mineralized scales that are composed of hierarchical assemblies of nano-crystals of calcium carbonate in the form of calcite. Coccolith formation in vivo occurs within compartmentalized mineralisation vesicles derived from the Golgi body...
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#1Anna DubankovaH-Index: 6
#2Vladimira HorovaH-Index: 1
Last.Evzen BouraH-Index: 23
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Abstract RNA-dependent RNA polymerase 3Dpol is a key enzyme for the replication of picornaviruses. The viral genome is translated into a single polyprotein that is subsequently proteolytically processed into matured products. The 3Dpol enzyme arises from a stable 3CD precursor that has high proteolytic activity but no polymerase activity. Upon cleavage of the precursor the newly established N-terminus of 3Dpol is liberated and inserts itself into a pocket on the surface of the 3Dpol enzyme. The ...
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#1Wangyu Liu (SCUT: South China University of Technology)H-Index: 8
#2Ling Zhang (SCUT: South China University of Technology)H-Index: 1
Last.Weigui Xie (SCUT: South China University of Technology)
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Abstract The accurate reconstruction model of plant microstructure is important for obtaining the mechanical properties of plant tissues. In this paper, a virtual segmentation technique is proposed to optimize Delaunay triangulation. Based on the optimized Delaunay triangulation, an Optimized Distance Weighted Tessellation (ODWT) algorithm is developed. Two different structures, namely carrot and retting maize vascular bundles, were reconstructed via the ODWT algorithm. The accuracy of ODWT is e...
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#1Pavel Tchelidze (European University)
#2H. Kaplan (URCA: University of Reims Champagne-Ardenne)H-Index: 6
Last.Marc Thiry (University of Liège)H-Index: 32
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Abstract Upstream binding transcription factor (UBTF) is a co-regulator of RNA polymerase I by constituting an initiation complex on rRNA genes. UBTF plays a role in rDNA bending and its maintenance in “open” state. It exists as two splicing variants, UBTF1 and UBTF2, which cannot be discerned with antibodies raised against UBTF. We investigated the ultrastructural localization of each variant in cells synthesizing GFP-tagged UBTF1 or UBTF2 by using anti-GFP antibodies and pre-embedding nanogold...
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#1Tristan Wagner (University of Paris)
#2Alexandra Boyko (MSU: Moscow State University)H-Index: 3
Last.Marco Bellinzoni (University of Paris)
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Abstract Mycobacterial KGD, the thiamine diphosphate (ThDP)-dependent E1o component of the 2-oxoglutarate dehydrogenase complex (OGDHC), is known to undergo significant conformational changes during catalysis with two distinct conformational states, previously named as the early and late state. In this work, we employ two phosphonate analogues of 2-oxoglutarate (OG), i.e. succinyl phosphonate (SP) and phosphono ethyl succinyl phosphonate (PESP), as tools to isolate the first catalytic steps and ...
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#1Lise Dauban (University of Toulouse)H-Index: 1
#2Alain Kamgoue (University of Toulouse)H-Index: 3
Last.Olivier Gadal (University of Toulouse)H-Index: 25
view all 7 authors...
Abstract Spatial organisation of chromosomes is a determinant of genome stability and is required for proper mitotic segregation. However, visualization of individual chromatids in living cells and quantification of their geometry, remains technically challenging. Here, we used live cell imaging to quantitate the three-dimensional conformation of yeast Saccharomyces cerevisiae ribosomal DNA (rDNA). rDNA is confined within the nucleolus and is composed of about 200 copies representing about 10% o...
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#1Fabian C. Herrmann (WWU: University of Münster)H-Index: 3
#2V Spiegler (WWU: University of Münster)H-Index: 4
Abstract Assessing the internal morphology of Caenorhabditis elegans by a topographical technique like atomic force microscopy (AFM) is a challenging process. As a prerequisite for a successful image acquisition, direct contact between the structure of interest and the AFM probe needs to be established. To gain this insight into the morphology of cuticle and intestine in C. elegans before and after treatment with a tannin-enriched hydro-ethanolic extract from Combretum mucronatum, we developed a...
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