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BMC Biotechnology
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2.30
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Papers 1446
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Published on Dec 1, 2019in BMC Biotechnology2.30
Kayla Carey (VT: Virginia Tech), Junghyun Ryu5
Estimated H-index: 5
(VT: Virginia Tech)
+ 4 AuthorsKiho Lee6
Estimated H-index: 6
(VT: Virginia Tech)
Background The CRISPR/Cas9 system can effectively introduce site-specific modifications to the genome. The efficiency is high enough to induce targeted genome modifications during embryogenesis, thus increasing the efficiency of producing genetically modified animal models and having potential clinical applications as an assisted reproductive technology. Because most of the CRISPR/Cas9 systems introduce site-specific double-stranded breaks (DSBs) to induce site-specific modifications, a major co...
Published on 2019in BMC Biotechnology2.30
Henrique César Teixeira Veras2
Estimated H-index: 2
(UnB: University of Brasília),
Christiane Gonçalves Campos2
Estimated H-index: 2
(UFG: Universidade Federal de Goiás)
+ 3 AuthorsNádia Skorupa Parachin8
Estimated H-index: 8
(UnB: University of Brasília)
Efficient xylose fermentation still demands knowledge regarding xylose catabolism. In this study, metabolic flux analysis (MFA) and metabolomics were used to improve our understanding of xylose metabolism. Thus, a stoichiometric model was constructed to simulate the intracellular carbon flux and used to validate the metabolome data collected within xylose catabolic pathways of non-Saccharomyces xylose utilizing yeasts. A metabolic flux model was constructed using xylose fermentation data from ye...
Published on 2019in BMC Biotechnology2.30
Aiyou Huang7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences),
Songcui Wu5
Estimated H-index: 5
(CAS: Chinese Academy of Sciences)
+ 3 AuthorsGuang-CeWANG22
Estimated H-index: 22
(CAS: Chinese Academy of Sciences)
Background Increasing CO2 emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, but have been mainly focused on growth and photosynthetic physiology. To acquire a thorough knowledge of the molecular regulation mechanisms, model species with clear genetic background should be selected ...
Published on Jan 9, 2019in BMC Biotechnology2.30
CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome. We tested two gene disruption methods in T. reesei using CRISPR/Cas9 in this study. The intracellularly expressed Cas9 led to unexpected off-target gene disruption in T. reesei QM9414, favoring inserting 9- or 12-bp at...
Published on Mar 8, 2019in BMC Biotechnology2.30
Guilherme Souza Prado2
Estimated H-index: 2
(UCB: Universidade Católica de Brasília),
Pingdwende Kader Aziz Bamogo (University of Montpellier)+ 10 AuthorsChristophe Brugidou2
Estimated H-index: 2
(University of Montpellier)
Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolution strategies have been used on genes encoding these proteins to generate thousands of variants with new or improved functions. The cotton boll weevil (Anthonomus grandis) is one of the major ...
Published on Dec 1, 2019in BMC Biotechnology2.30
Michela Flego8
Estimated H-index: 8
(ISS: Istituto Superiore di Sanità),
Aldo Frau1
Estimated H-index: 1
(University of Cagliari)
+ 7 AuthorsEnzo Tramontano25
Estimated H-index: 25
(University of Cagliari)
Ebola hemorrhagic fever is caused by the Ebola filovirus (EBOV), which is one of the most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subversion of both the innate and adaptive host immune response. The multifunctional viral VP35 protein is involved in this process by exerting an antagonistic action against the early antiviral alpha/beta interferon (IFN-α/β) response, and represents a suitable target for the development of st...
Published on Jan 10, 2019in BMC Biotechnology2.30
Matthew P. A. Bellefleur1
Estimated H-index: 1
(UF: University of Florida),
Soo-Young Wanda1
Estimated H-index: 1
(UF: University of Florida),
Roy Curtiss7
Estimated H-index: 7
(UF: University of Florida)
Background Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic organisms live in environments that are often nutrient limited; therefore, the genome of these organisms encodes far fewer proteins used for efflux of chemicals when compared to members of the Enterobacteriaceae family. Understanding efflux mechanisms can lead to a greater effic...
Published on Jan 28, 2019in BMC Biotechnology2.30
Guiqi Zhang1
Estimated H-index: 1
,
Yi Liu1
Estimated H-index: 1
+ 3 AuthorsWeibing Xu1
Estimated H-index: 1
Background Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS.
Published on Dec 1, 2019in BMC Biotechnology2.30
Jian Pang1
Estimated H-index: 1
(Inner Mongolia University of Technology),
Junshu Wang (SDU: Shandong University)+ 2 AuthorsQingsheng Qi25
Estimated H-index: 25
(SDU: Shandong University)
Background In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-understand E. coli and extend its application in industry.
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