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BMC Biotechnology
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2.60
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1411
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Published on Jan 10, 2019in BMC Biotechnology 2.60
Matthew P. A. Bellefleur1
Estimated H-index: 1
(UF: University of Florida),
Soo-Young Wanda1
Estimated H-index: 1
(UF: University of Florida),
Roy Curtiss7
Estimated H-index: 7
(UF: University of Florida)
Background Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic organisms live in environments that are often nutrient limited; therefore, the genome of these organisms encodes far fewer proteins used for efflux of chemicals when compared to members of the Enterobacteriaceae family. Understanding efflux mechanisms can lead to a greater effic...
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Published on Jan 9, 2019in BMC Biotechnology 2.60
Background CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome.
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Published on Mar 8, 2019in BMC Biotechnology 2.60
Guilherme Souza Prado2
Estimated H-index: 2
(UCB: Universidade Católica de Brasília),
Pingdwende Kader Aziz Bamogo (University of Montpellier)+ 10 AuthorsChristophe Brugidou2
Estimated H-index: 2
(University of Montpellier)
Background Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To extend the spectrum of targeted pathogens and overtake the development of resistance, molecular evolution strategies have been used on genes encoding these proteins to generate thousands of variants with new or improved functions. The cotton boll weevil (Anthonomus grandis) is one of...
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Published on Jan 28, 2019in BMC Biotechnology 2.60
Guiqi Zhang1
Estimated H-index: 1
,
Yi Liu1
Estimated H-index: 1
+ 3 AuthorsWeibing Xu1
Estimated H-index: 1
Background Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS.
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Published on Mar 20, 2019in BMC Biotechnology 2.60
Soumaya Najah2
Estimated H-index: 2
(Université Paris-Saclay),
Corinne Saulnier1
Estimated H-index: 1
(Université Paris-Saclay)
+ 1 AuthorsStéphanie Bury-Moné1
Estimated H-index: 1
(Université Paris-Saclay)
Background The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editing is expanding, some limitations remain such as possible off-target effects and design constraints. These are compounded when performing systematic genome editing at distinct loci or when targeting repeated sequences (e.g. multicopy genes or mobile genetic elements). To overcome...
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Published on Jan 28, 2019in BMC Biotechnology 2.60
Background Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system has appeared a promising tool in site-directed mutagenesis due to its innovative utilization in different branches of biology. CRISPR-Cas9 nuclease system have supersedes all previously existed strategies and their associated pitfalls encountered with site-specific mutagenesis.
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