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Biotechnology Journal
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3.54
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2539
Papers 2610
1 page of 261 pages (2,610 results)
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#1Francis McEachern (McGill University)
#2Edward J. Harvey (McGill University)H-Index: 22
Last. Geraldine Merle (École Polytechnique de Montréal)
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Infections are a huge economic liability to the health care system, although real time detection could allow early treatment protocols to avoid some of this cost and patient morbidity and mortality. Pseudomonas aeruginosa (PA) is a drug-resistant gram-negative bacterium found ubiquitously in clinical settings, accounting for up to 27% of hospital acquired infections. PA secretes a vast array of molecules, ranging from secondary metabolites to quorum sensing molecules, of which many can be exploi...
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#1Eric S. Fan (NTHU: National Tsing Hua University)
#2Ken W. Lu (NTHU: National Tsing Hua University)H-Index: 1
Last. Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
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Photosynthetic generation of reducing power makes cyanobacteria an attractive host for biochemical reduction compared to cell-free and heterotrophic systems, which require burning of additional resources for the supply of reducing equivalent. Here, using xylitol synthesis as an example, we demonstrated efficient uptake and reduction of xylose photoautotrophically in Synechococcus elongatus PCC7942 upon introduction of an effective xylose transporter from Escherichia coli (Ec-XylE) and the NADPH-...
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#1Naoya Nochino (Osaka University)
#2Yoshihiro Toya (Osaka University)H-Index: 12
Last. Hiroshi Shimizu (Osaka University)H-Index: 37
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Oxygen supply is one of the major factors determining the metabolic state in microorganisms, and it affects the productivity of various compounds during bioproduction. In Escherichia coli, the expression levels of numerous metabolic genes are regulated by transcription factors in response to changes in environmental oxygen conditions. Even at a single concentration of dissolved oxygen, the amount of available oxygen can change depending on the oxygen transfer coefficient. However, it is not know...
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#1Marion Peyressatre (University of Montpellier)H-Index: 1
#2Arthur Laure (University of Montpellier)
Last. May C. Morris (University of Montpellier)H-Index: 28
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CDK5 plays a major role in neuronal functions, and is hyperactivated in neurodegenerative pathologies as well as in glioblastoma and neuroblastoma. Although this kinase constitutes an established biomarker and pharmacological target, there are few means of probing its activity in cell extracts or in living cells. To this aim we engineered a fluorescent peptide reporter of CDK5 kinase activity, derived from a library of CDK5-specific substrates, and established its ability to respond to recombina...
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#1Irisappan Ganesh (POSTECH: Pohang University of Science and Technology)H-Index: 6
#1Irisappan Ganesh (POSTECH: Pohang University of Science and Technology)
Last. Jeong Wook Lee (POSTECH: Pohang University of Science and Technology)
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Gas molecules are ubiquitous in the environment and are used as nutrient and energy sources for living organisms. Many organisms, therefore, have developed gas-sensing systems to respond efficiently to changes in the atmospheric environment. In microorganisms and plants, two-component systems (TCSs) and transcription factors (TFs) are two primary mechanisms to sense gas molecules. In this review, we discuss gas-sensing transcriptional regulators, TCSs and TFs, focusing on protein structures, mec...
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#1Yingjun FanH-Index: 1
Last. Haiying HangH-Index: 19
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#1He-Lin Xu (WMU: Wenzhou Medical College)H-Index: 8
#2Wai-Geng Yang (WMU: Wenzhou Medical College)H-Index: 1
Last. Ying-Zheng Zhao (WMU: Wenzhou Medical College)H-Index: 21
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Toad bone not only rich in cartilage-like matrix components including collagen II and glycosaminoglycans but also presents low immunogenicity because of its much less developed evolution. It was inferred that decellularized matrix of toad cartilage (dBECM) may provide the more profitable osteoinductive microenvironment for mesenchymal stem cells (MSCs) to promote the repair of critical-size bone defects. Herein, a hollow bone-inspired tube was firstly prepared by using the bone matrix component,...
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#1Danila Boytsov (Johannes Kepler University of Linz)H-Index: 1
#2Christof Hannesschlaeger (Johannes Kepler University of Linz)H-Index: 3
Last. Peter Pohl (Johannes Kepler University of Linz)H-Index: 34
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Measurements of the unitary hydraulic conductivity of membrane channels, pf , may be hampered by difficulties in producing sufficient quantities of purified and reconstituted proteins. Low yield expression, the purely empiric choice of detergents, as well as protein aggregation and misfolding during reconstitution may result in an average of less than one reconstituted channel per large unilamellar vesicle. This limits their applicability for pf measurements, independent of whether light scatter...
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#1Ana C. A. RoqueH-Index: 21
#2Ana Sofia PinaH-Index: 7
Last. Marcel Ottens (TU Delft: Delft University of Technology)H-Index: 20
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While packed bed chromatography, known as conventional chromatography, has been serving the biopharmaceutical industry for decades as the bioseparation method of choice, alternative approaches are likely to take an increasing leading role in the next few years. The high number of new biological drugs under development, and the need to make biopharmaceuticals widely accessible, has been driving the academia and industry in the quest of anything but conventional chromatography approaches. In this ...
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