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Journal of Immunological Methods
IF
2.19
Papers
12046
Papers 12048
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Published on Jan 1, 2019in Journal of Immunological Methods 2.19
Yang Song (Tsinghua University), Julie Nigro (University of Melbourne)+ 4 AuthorsKumar Visvanathan27
Estimated H-index: 27
(University of Melbourne)
Abstract Cytokine production by human peripheral blood mononuclear cells including monocytes, is frequently assessed by measuring secreted cytokines using e nzyme l inked i mmuno s orbent a ssay (ELISA), whereby the total concentration of one cytokine of interest is obtained without information regarding the cell type responsible for making the cytokine. Cytokines can be retained inside the cell using protein transport inhibitors. Subsequent analysis by flow cytometry not only identifies the cel...
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Published on Jan 1, 2019in Journal of Immunological Methods 2.19
Nami Iwamoto (Vaccine Research Center), Rosemarie Mason1
Estimated H-index: 1
(Vaccine Research Center)
+ 5 AuthorsMario Roederer90
Estimated H-index: 90
(Vaccine Research Center)
Abstract An effective prophylactic vaccine against human immunodeficiency virus (HIV) will likely require a potent antibody response that can neutralize the virus at the mucosal portal of entry. The elicitation of potent broadly-neutralizing anti-sera will be an iterative process, optimizing candidates that only block a fraction of potential viral strains. This effect, termed “sieving”, is evidence of a partially efficacious vaccine. Understanding the mechanisms of resistance of the breakthrough...
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Published on Dec 1, 2018in Journal of Immunological Methods 2.19
Laura M. De Plano3
Estimated H-index: 3
(University of Messina),
Enza Fazio19
Estimated H-index: 19
(University of Messina)
+ 5 AuthorsSalvatore P.P. Guglielmino10
Estimated H-index: 10
(University of Messina)
Abstract Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. In this study, from random M13 phage display libraries, we selected phage clones that specifically bind surface of Staph...
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Published on Jan 1, 2019in Journal of Immunological Methods 2.19
Cemile Sönmez3
Estimated H-index: 3
,
Figen Sezen , Selcuk Kilic (University of Health Sciences Antigua)
Abstract Background Serological methods have great importance for the detection of Treponema pallidum antibodies in syphilis diagnosis. The goal of the present study is to evaluate various commercially available screening assays in comparison with the FTA-abs test. Methods A total of 363 serum samples were enrolled in the study. Following routine testing including RPR and TPHA tests, each sample was tested by treponemal immunoassays (Chorus Syphilis Screen Recombinant, Architect Syphilis TP, Syp...
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Published on Nov 1, 2018in Journal of Immunological Methods 2.19
Laura Kofoed Hvidsten Ørstrup1
Estimated H-index: 1
(University of Copenhagen),
Rita Slaaby5
Estimated H-index: 5
(Novo Nordisk)
+ 10 AuthorsThomas Pedersen10
Estimated H-index: 10
(Novo Nordisk)
Abstract Translation across species of immunoassay results is often challenging due to the lack of cross-species reactivity of antibodies. In order to investigate the biology of insulin and IGF1 receptors, we generated new versatile monoclonal assay antibodies using the extracellular domain of the insulin/IGF1 hybrid receptor as the bait protein in the Adimab yeast antibody discovery platform and as the antigen in a rabbit monoclonal antibody platform. The resulting antibody clones were screened...
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