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#2Joanna KlimH-Index: 2
Last. Urszula ZielenkiewiczH-Index: 12
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Abstract Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids of different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry a diverse number of genes, allowing the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. The present study characterizes the plasmidome generated from an arsenic-resistant strain named ZS207, classified as Acinet...
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#1Eve Elken (UT: University of Tartu)
#2Eeva Heinaru (UT: University of Tartu)H-Index: 13
Last. Ain Heinaru (UT: University of Tartu)H-Index: 19
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Several years ago, a laboratory-constructed plasmid with a single-component phenol monooxygenase gene (pheBA operon) flanked by two IS elements was released to a phenol-polluted area. During the following years, we found in the test area widely distributed pheBA operon-containing bacteria. The new pheBA(+) strains belong predominantly to the Pseudomonas fluorescens group, and they did not arise via selection of the released PHE plasmid. On the contrary, the formation of several different types o...
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#1Marta Rozwandowicz (UU: Utrecht University)H-Index: 2
#2Joost Hordijk (UU: Utrecht University)H-Index: 12
Last. Michael S. M. BrouwerH-Index: 11
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Plasmid incompatibility is the inability of two plasmids to be stably maintained in one cell, resulting in loss of one of the plasmids in daughter cells. Dislodgement is a phenotypically distinct form of incompatibility, described as an imperfect reproduction, manifesting in rapid exclusion of a resident plasmid after superinfection. The relationship between plasmids of the phenotypic incompatibility groups IncB/O and IncZ is unclear. Their inability to co-exist was initially referred to as disl...
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#1Patricia ApuraH-Index: 3
#2Margarida SaramagoH-Index: 7
Last. Susana DominguesH-Index: 10
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Abstract Pseudomonas putida is a highly attractive production system for industrial needs; however, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for different purposes. Due to the small RNAs modular composition, design facilities and ability to tune gene expression, they constitute a powerful tool in genetic and metabolic...
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#1Masahiro Suzuki (Fujita Health University)H-Index: 10
#2Yohei Doi (Fujita Health University)H-Index: 50
Last. Yoshichika Arakawa (Nagoya University)H-Index: 55
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Abstract Objectives Systematic comparison of multiple plasmids remains challenging. We aimed to develop a new method for phylogenetic analysis of plasmids, open reading frame (ORF)-based binarized structure network analysis of plasmids (OSNAp). Methods With the OSNAp, the genetic structures of plasmids in a given plasmid group are expressed as binary sequences based on the presence or absence of ORFs regardless of their positions or directions. As a proof-of-concept, ORFs were collected from 101...
1 CitationsSource
#1Jananee Jaishankar (IITD: Indian Institute of Technology Delhi)H-Index: 1
#1Jananee JaishankarH-Index: 1
Last. Preeti SrivastavaH-Index: 6
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Abstract The Gram-negative bacterium Escherichia coli has been the work horse for recombinant protein production since the past several years. However, most of the gene expression systems used either require expensive inducers or exhibit low strength. In the present study, we have generated a strong promoter by repeated rounds of random mutagenesis in a stationary phase promoter isolated from Gordonia sp. IITR100. The promoter activity increased 16-fold as compared to the wild-type promoter. The...
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#1Xingyu Kang (HAU: Huazhong Agricultural University)
#2Chunqiu Li (HAU: Huazhong Agricultural University)
Last. Yi Luo (HAU: Huazhong Agricultural University)
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Abstract In this study, a cryptic plasmid from Aeromonas hydrophila (pAhX22) was cloned and characterized. pAhX22 was 2523 bp long, had a GC content of 59.9%, and contained two putative open reading frames (ORFs). orf1 and orf2 encoded putative proteins of 458 amino acids and 88 amino acids, respectively; these putative proteins might be involved in plasmid replication. An Escherichia coli–A. hydrophila shuttle vector, pAEsv-1 (4587 bp, KanR), was constructed using in-fusion cloning, combining p...
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#2Alice E. Beddis (Imperial College London)H-Index: 1
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Abstract Plasmids transfer at highly variable rates that spread over 10 orders of magnitude. While rates have been measured for decades and it is known that the rates are affected bysome biotic and abiotic factors, it is unclear how and to what extent these factors determine the rates of transfer. We performed a meta-analysis of 1224 published transfer rates from 33 papers (filtered to 612 transfer rates) to assess this variation. Over three quarters of the variation can be predicted, with plasm...
1 CitationsSource
#1Sarah A. Revitt-Mills (DI: Discovery Institute)H-Index: 2
#2Carmen Lao (DI: Discovery Institute)
Last. Vicki Adams (DI: Discovery Institute)H-Index: 9
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Abstract Many of the disease-causing toxins of the pathogenic bacterium Clostridium perfringens are harboured on large, highly stable, conjugative plasmids. Previous work has established the requirement of a ParMRC-like partitioning system for plasmid maintenance, but little is known about other mechanisms used to ensure stable plasmid inheritance. The archetypal 47 kb Tcp plasmid, pCW3, encodes a gene, resP, whose putative product has sequence similarity to members of the serine recombinase fam...
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#1Claire de Curraize (USYD: University of Sydney)H-Index: 1
#2Eliane SieborH-Index: 14
Last. M HallRuth (USYD: University of Sydney)H-Index: 53
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Abstract Several groups of integrative mobilizable elements (IMEs) have been found at the 3′-end of the chromosomal trmE gene, all harbouring a class 1 integron. Here, a new IME, Salmonella Genomic Island 0 (SGI0), was found in trmE in a French clinical Proteus mirabilis strain. SGI0 was characterized by the lack of class 1 integron. SGI0 is closely related to the backbones of SGI1 (97.59% nucleotide identity) and SGI2 (97.72% nucleotide identity). SGI0 harboured also the AcaCD binding sites and...
2 CitationsSource
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