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#1Richard J. Meinersmann (ARS: Agricultural Research Service)H-Index: 27
Abstract IncI2 type plasmids are medium-sized (~55–80 kb) conjugative plasmids that have been found carrying important antimicrobial resistance genes but have also been frequently found as cryptic plasmids. The DNA sequences for 147 fully sequenced IncI2 plasmids were studied by a whole-plasmid multi-locus sequence typing (wpMLST) scheme. A total of 171 loci were identified of which 52 were considered core (carried by greater than 95% of the plasmids). Most of the plasmids carrying the antimicro...
#1Estefanía Morales-Ruiz (IPN: Instituto Politécnico Nacional)
#2Astrid López-Ceballos (IPN: Instituto Politécnico Nacional)
Last.Ignacio E. Maldonado-Mendoza (IPN: Instituto Politécnico Nacional)H-Index: 20
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Abstract Bacterial transformation is a crucial step in the genetic manipulation of a bacterium. However, Gram-positive bacteria are difficult to transform and consequently many different methodologies have been developed. Here, we examined the transformation efficiencies of an electroporation protocol by varying three main factors: the composition of the electroporation buffer, the strength of the electric pulse, and the composition of the recovery media. Overall, transformation efficiency was e...
#1Yu Zhang (Sichuan University)
#2Chang-Wei Lei (Sichuan University)H-Index: 8
Last.Hongning Wang (Sichuan University)H-Index: 15
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Abstract The multiresistance gene cfr has a broad host range encompassing both Gram-positive and Gram-negative bacteria, and can be located on the chromosomes or on plasmids. In this study, a novel conjugative plasmid carrying cfr, designated as pPvSC3, was characterized in a Proteus vulgaris strain isolated from swine in China. Plasmid pPvSC3 is 284,528 bp in size and harbors 10 other antimicrobial resistance genes, making it a novel plasmid that differs from all known plasmids due to its uniqu...
#1Robert A. Moran (USYD: University of Sydney)H-Index: 5
#2M HallRuth (USYD: University of Sydney)H-Index: 51
Abstract The IncB/O multiresistance plasmid R16, recovered in 1956 and used in experimental studies of B/O plasmid features, was sequenced. The resistance genes are all within a class 1 integron closely related to In1 containing the cassette array oxa2 - aadA1 - oxa2 -orfD and sul1 in the 3′-conserved segment (3′-CS), with Tn 10 , containing tetA (B), inserted just inside the 3′-CS. The integron is part of a 25,144 bp region inserted in the plasmid backbone, bounded on only one side by a truncat...
#1Shao-Heng Bao (National University of Defense Technology)
#2Dong-Yi Zhang (Hunan University of Science and Technology)
Last.Er Meng (Hunan University of Science and Technology)
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Abstract Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techniques to produce a variety of heterologous products, including pinene. Here, we investigated the stability of genetic circuits encoding the pinene producing pathway during f...
#1Wencui Sun (Peking Union Medical College)H-Index: 1
#2Jiawen Teng (Peking Union Medical College)
Last.Guohui Bian (Peking Union Medical College)H-Index: 2
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Abstract Eukaryotic inducible overexpression systems, including Tet-On and mifepristone-inducible systems, have been widely used to study gene functions by reverse genetics. Among the transposon systems reported to date, the piggyBac transposon system is one of the most efficient in cultured mammalian cells. Here, we report a piggyBac -based double-inducible system that combined the advantages of previous systems. To create this system, the trans- and cis-elements of the Tet-On and mifepristone-...
#1François Berthold (UDS: University of Strasbourg)H-Index: 3
#2David Roujol (University of Toulouse)H-Index: 5
Last.Corinne Schmitt-Keichinger (UDS: University of Strasbourg)H-Index: 8
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Abstract Transient expression of proteins based on agro-infiltration techniques has proven very efficient and straightforward to study the intrinsic properties of proteins. The level of protein expression has been enhanced by the use of vector plasmids containing virus-derived sequences and the cloning step has been facilitated by recombination technologies. The pEAQ-HT-DEST series of vectors fulfilling these improvements are vectors of choice. However, they lack the possibility to directly and ...
#1Jin Yang Baek (SMC: Samsung Medical Center)H-Index: 15
#2Sun Young Cho (SMC: Samsung Medical Center)H-Index: 26
Last.Kwan Soo Ko (SMC: Samsung Medical Center)H-Index: 43
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Abstract Recently, Escherichia coli isolates co-producing New Delhi metallo-β-lactamase (NDM)-5 and oxacillinase (OXA)-181 were identified in a tertiary-care hospital of South Korea. Isolate CC1702–1 was collected from urine in January 2017 and isolate CC1706-1 was recovered from a transtracheal aspirate of a hospitalized patient in May 2017. Carbapenemase genes were identified by multiplex PCR and sequencing, and whole genome sequencing was performed subsequently using the PacBio RSII system. B...
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