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Nature Structural & Molecular Biology
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12.11
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Papers 5727
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#1Seychelle M. Vos (MIT: Massachusetts Institute of Technology)
#2Lucas Farnung (MPG: Max Planck Society)H-Index: 6
Last. Patrick Cramer (MPG: Max Planck Society)H-Index: 63
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Transcription by RNA polymerase II (Pol II) is carried out by an elongation complex. We previously reported an activated porcine Pol II elongation complex, EC*, encompassing the human elongation factors DSIF, PAF1 complex (PAF) and SPT6. Here we report the cryo-EM structure of the complete EC* that contains RTF1, a dissociable PAF subunit critical for chromatin transcription. The RTF1 Plus3 domain associates with Pol II subunit RPB12 and the phosphorylated C-terminal region of DSIF subunit SPT5....
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#1Christine Röder (HHU: University of Düsseldorf)H-Index: 1
#2Tatsiana Kupreichyk (HHU: University of Düsseldorf)H-Index: 1
Last. Gunnar F. Schröder (HHU: University of Düsseldorf)H-Index: 31
view all 9 authors...
Amyloid deposits consisting of fibrillar islet amyloid polypeptide (IAPP) in pancreatic islets are associated with beta-cell loss and have been implicated in type 2 diabetes (T2D). Here, we applied cryo-EM to reconstruct densities of three dominant IAPP fibril polymorphs, formed in vitro from synthetic human IAPP. An atomic model of the main polymorph, built from a density map of 4.2-A resolution, reveals two S-shaped, intertwined protofilaments. The segment 21-NNFGAIL-27, essential for IAPP amy...
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#1Tao Wu (Harvard University)
#2Hojong Yoon (Harvard University)H-Index: 4
Last. Eric S. Fischer (Harvard University)H-Index: 16
view all 6 authors...
Controlled perturbation of protein activity is essential to study protein function in cells and living organisms. Small molecules that hijack the cellular protein ubiquitination machinery to selectively degrade proteins of interest, so-called degraders, have recently emerged as alternatives to selective chemical inhibitors, both as therapeutic modalities and as powerful research tools. These systems offer unprecedented temporal and spatial control over protein function. Here, we review recent de...
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#1Xiaochun Xiong (UofU: University of Utah)H-Index: 1
#2John Gerbrandt Tasman Menting (University of Melbourne)
Last. Simon J. Fisher (UofU: University of Utah)H-Index: 2
view all 19 authors...
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#1Jong Woo Bae (SNU: Seoul National University)
#2S. Chul Kwon (SNU: Seoul National University)H-Index: 7
Last. Jong-Seo Kim (SNU: Seoul National University)H-Index: 15
view all 5 authors...
RNA-binding sites (RBSs) can be identified by liquid chromatography and tandem mass spectrometry analyses of the protein–RNA conjugates created by crosslinking, but RBS mapping remains highly challenging due to the complexity of the formed RNA adducts. Here, we introduce RBS-ID, a method that uses hydrofluoride to fully cleave RNA into mono-nucleosides, thereby minimizing the search space to drastically enhance coverage and to reach single amino acid resolution. Moreover, the simple mono-nucleos...
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#1Li-Qiang Wang (WHU: Wuhan University)H-Index: 1
#2Kun Zhao (CAS: Chinese Academy of Sciences)H-Index: 27
Last. Jie Chen (WHU: Wuhan University)H-Index: 24
view all 15 authors...
Prion diseases are caused by the misfolding of prion protein (PrP). Misfolded PrP forms protease-resistant aggregates in vivo (PrPSc) that are able to template the conversion of the native form of the protein (PrPC), a property shared by in vitro–produced PrP fibrils. Here we produced amyloid fibrils in vitro from recombinant, full-length human PrPC (residues 23–231) and determined their structure using cryo-EM, building a model for the fibril core comprising residues 170−229. The PrP fibril con...
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#1Vittorio SartorelliH-Index: 49
#2Shannon M. Lauberth (UCSD: University of California, San Diego)H-Index: 6
Noncoding RNAs (ncRNAs) direct a remarkable number of diverse functions in development and disease through their regulation of transcription, RNA processing and translation. Leading the charge in the RNA revolution is a class of ncRNAs that are synthesized at active enhancers, called enhancer RNAs (eRNAs). Here, we review recent insights into the biogenesis of eRNAs and the mechanisms underlying their multifaceted functions and consider how these findings could inform future investigations into ...
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#1J. Cheng (LMU: Ludwig Maximilian University of Munich)H-Index: 7
#2Nikola Kellner (Heidelberg University)H-Index: 3
Last. Roland Beckmann (LMU: Ludwig Maximilian University of Munich)H-Index: 48
view all 5 authors...
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#1Xiaochun Xiong (UofU: University of Utah)H-Index: 1
#2John Gerbrandt Tasman Menting (University of Melbourne)
Last. Simon J. Fisher (UofU: University of Utah)H-Index: 2
view all 19 authors...
Human insulin and its current therapeutic analogs all show propensity, albeit varyingly, to self-associate into dimers and hexamers, which delays their onset of action and makes blood glucose management difficult for people with diabetes. Recently, we described a monomeric, insulin-like peptide in cone-snail venom with moderate human insulin-like bioactivity. Here, with insights from structural biology studies, we report the development of mini-Ins—a human des-octapeptide insulin analog—as a str...
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#1Xiangdong Zheng (Columbia University)
#2Ziao Fu (Columbia University)H-Index: 4
Last. Zhenning Ren (BCM: Baylor College of Medicine)H-Index: 2
view all 16 authors...
Cyclic nucleotide–gated (CNG) channels convert cyclic nucleotide (CN) binding and unbinding into electrical signals in sensory receptors and neurons. The molecular conformational changes underpinning ligand activation are largely undefined. We report both closed- and open-state atomic cryo-EM structures of a full-length Caenorhabditis elegans cyclic GMP−activated channel TAX-4, reconstituted in lipid nanodiscs. These structures, together with computational and functional analyses and a mutant ch...
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