(Ludwig Institute for Cancer Research), Yaping Liu (UC: University of Cincinnati)+ 5 AuthorsBing Ren82
Estimated H-index: 82
(UCSD: University of California, San Diego)
We report a molecular assay, Methyl-HiC, that can simultaneously capture the chromosome conformation and DNA methylome in a cell. Methyl-HiC reveals coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus, and delineates heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. It enables simultaneous characterization of cell-type-specific chromatin organization and epigenome in complex tissues.
(UTSW: University of Texas Southwestern Medical Center), Erik S. Welf14
Estimated H-index: 14
(UTSW: University of Texas Southwestern Medical Center)+ 4 AuthorsGaudenz Danuser59
Estimated H-index: 59
(UTSW: University of Texas Southwestern Medical Center)
Rapid developments in live-cell three-dimensional (3D) microscopy enable imaging of cell morphology and signaling with unprecedented detail. However, tools to systematically measure and visualize the intricate relationships between intracellular signaling, cytoskeletal organization and downstream cell morphological outputs do not exist. Here, we introduce u-shape3D, a computer graphics and machine-learning pipeline to probe molecular mechanisms underlying 3D cell morphogenesis and to test the in...