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Nassapat Boonvitthya
Chulalongkorn University
6Publications
2H-index
44Citations
Publications 6
Newest
#1Chanika OuephanitH-Index: 2
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Most common industrial xylanases are produced from filamentous fungi. In this study, the codon-optimized xynA gene encoding xylanase A from the fungus Penicilium citrinum was successfully synthesized and expressed in the yeast Pichia pastoris. The levels of secreted enzyme activity under the control of glyceraldehyde-3-phosphate dehydrogenase (PGAP) and alcohol oxidase 1 (PAOX1) promoters were compared. The Pc Xyn11A was produced as a soluble protein and the total xylanase activity under the con...
#1Chanika Ouephanit (Chula: Chulalongkorn University)H-Index: 2
Last.Warawut Chulalaksananukul (Chula: Chulalongkorn University)H-Index: 11
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Abstract Filamentous fungi are the most common industrial xylanase producers. In this study, the xynA gene encoding xylanase A of Penicilium citrinum was successfully synthesized and expressed in Yarrowia lipolytica under the control of the strong constitutive TEF promoter. Native and preproLIP2 secretion signals were used for comparison of the expression and secretion level. The recombinant xylanase was produced as a soluble protein, and the total activity production reached 11 and 52 times hig...
#1Phawadee Buathong (Chula: Chulalongkorn University)H-Index: 1
Last.Warawut Chulalaksananukul (Chula: Chulalongkorn University)H-Index: 11
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Abstract 1,12-Dodecanedioic acid (DDA), a primary compound and an intermediate precursor for various chemical products, is normally produced by chemical synthesis, which presents potential disadvantages. Instead, the biosynthesis of 1,12-DDA by recombinant (r) microorganisms may offer a viable production route. Cytochrome P450 (CYP) can terminally oxidize fatty acids to ω-hydroxy-fatty acids and further to dicarboxylic acids (ω-oxidation). The wild type (r) CYP52A17 LL and its engineered L261S/L...
The suitability of crude glycerol (75% (v/v) glycerol content) from a biodiesel production plant, and soybean meal and rice bran hydrolysates as replacements in the culture medium for commercially available glycerol, peptone and yeast extract, respectively, to form a low cost culture medium for the growth of, and recombinant endoglucanase II production by, Pichia pastoris was evaluated. The use of 1% (v/v) crude glycerol as a carbon source, and a mixture of 0.175% (v/v) soybean meal and 0.15% (v...
#1Nassapat Boonvitthya (Chula: Chulalongkorn University)H-Index: 2
#2Jittra Piapukiew (Chula: Chulalongkorn University)H-Index: 7
Last.Warawut Chulalaksananukul (Chula: Chulalongkorn University)H-Index: 11
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Jatropha curcas L. (Physic nut) is a drought-resistant shrub belonged to the family Euphorbiaceae. The oil from most J. curcas varieties has a low viscosity that makes it an attractive alternative energy source for biofuel production. Evaluating the degree of genetic diversity between populations and the development of genome wide markers are important for the long-term improvement of cultured varieties in any given habitat. The genetic diversity of 58 samples from 13 J. curcas populations, and ...
#1Nassapat Boonvitthya (Chula: Chulalongkorn University)H-Index: 2
#2Sophie Bozonnet (University of Toulouse)H-Index: 8
Last.Warawut Chulalaksananukul (Chula: Chulalongkorn University)H-Index: 11
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The sequences encoding the genes for endoglucanase II and cellobiohydrolase II from the fungus Trichoderma reesei QM9414 were successfully cloned and expressed in Yarrowia lipolytica under the control of the POX2 or TEF promoters, and using either the native or preproLip2 secretion signals. The expression level of both recombinant enzymes was compared with that obtained using Pichia pastoris, under the control of the AOX1 promoter to evaluate the utility of Y. lipolytica as a host strain for rec...
#1Nassapat Boonvitthya (Chula: Chulalongkorn University)H-Index: 2
#2Phatrapan Tanapong (Chula: Chulalongkorn University)H-Index: 1
Last.Warawut Chulalaksananukul (Chula: Chulalongkorn University)H-Index: 11
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The glucan 1,3-beta-glucosidase A gene (exgA) from Aspergillus oryzae and fused to the Saccharomyces cerevisiae signal peptide (α-factor) was expressed under the control of either a constitutive (GAP) or an inducible (AOX1) promoter in Pichia pastoris. A 1.4-fold higher extracellular enzyme activity (2 U/ml) was obtained using the AOX1 inducible expression system than with the GAP constitutive promoter (1.4 U/ml). The purified recombinant ExgA enzyme, with a yield of 10 mg protein/l culture supe...
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