Osamu Nureki
University of Tokyo
Publications 555
#1Masaru SasakiH-Index: 1
#2Hanako NishikawaH-Index: 2
Last.KenichinishiyamaH-Index: 21
view all 13 authors...
1 CitationsSource
Abstract Xenobiotic and metabolite extrusion is an important process for the proper functions of cells and their compartments, including acidic organelles. MATE (multidrug and toxic compound extrusion) is a large family of secondary active transporters involved in the transport of various compounds across cellular and organellar membranes, and is present in the three domains of life. The major substrates of the bacterial MATE transporters are cationic compounds, including clinically important an...
Recent studies have shown that stem cell memory T (TSCM) cell-like properties are important for successful adoptive immune therapy by the chimeric antigen receptor-engineered-T (CAR-T) cells. We previously reported that both human and murine activated T cells are converted into stem cell memory-like T (iTSCM) cells by co-culture with stromal OP9 cells expressing the NOTCH-ligand. However, the mechanism of NOTCH-mediated iTSCM reprogramming remains to be elucidated. Here, we report that the NOTCH...
1 CitationsSource
#1Kotaro KoiwaiH-Index: 1
#2Jun Tsukimoto (Biotechnology Institute)H-Index: 1
Last.Fumiaki YumotoH-Index: 12
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In cellulo crystallization is a developing technique to provide crystals for protein structure determination, particularly for proteins that are difficult to prepare by in vitro crystallization. This method has a key advantage: it requires neither a protein purification step nor a crystallization step. However, there is still no systematic strategy for improving the technique of in cellulo crystallization because the process occurs spontaneously. Here we report a protocol to produce and extract ...
#1Norimichi Nagano (UTokyo: University of Tokyo)
#2Megumi Honjo (UTokyo: University of Tokyo)H-Index: 5
Last.Makoto Aihara (UTokyo: University of Tokyo)H-Index: 38
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Summary Transmembrane channel-like (TMC) 1 and 2 are required for the mechanotransduction of mouse inner ear hair cells and localize to the site of mechanotransduction in mouse hair cell stereocilia. However, it remains unclear whether TMC1 and TMC2 are indeed ion channels and whether they can sense mechanical force directly. Here we express TMC1 from the green sea turtle (CmTMC1) and TMC2 from the budgerigar (MuTMC2) in insect cells, purify and reconstitute the proteins, and show that liposome-...
1 CitationsSource
#1Wataru Shihoya (UTokyo: University of Tokyo)H-Index: 4
#2Keiichi InoueH-Index: 21
Last.Osamu Nureki (UTokyo: University of Tokyo)H-Index: 56
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Heliorhodopsins (HeRs) are a family of rhodopsins that was recently discovered using functional metagenomics1. They are widely present in bacteria, archaea, algae and algal viruses2,3. Although HeRs have seven predicted transmembrane helices and an all-trans retinal chromophore as in the type-1 (microbial) rhodopsin, they display less than 15% sequence identity with type-1 and type-2 (animal) rhodopsins. HeRs also exhibit the reverse orientation in the membrane compared with the other rhodopsins...
1 CitationsSource
#1M. Hiraizumi (Mitsubishi Tanabe Pharma)H-Index: 2
#2Keitaro Yamashita (UTokyo: University of Tokyo)H-Index: 2
Last.Osamu Nureki (UTokyo: University of Tokyo)H-Index: 56
view all 4 authors...
In eukaryotic membranes, P4-ATPases mediate the translocation of phospholipids from the outer to inner leaflet and maintain lipid asymmetry, which is critical for membrane trafficking and signaling pathways. Here we report the cryo-EM structures of six distinct intermediates of the human ATP8A1-CDC50a hetero-complex, at 2.6–3.3 A resolutions, elucidating lipid translocation cycle of this P4-ATPase. ATP-dependent phosphorylation induces a large rotational movement of the actuator domain around th...
2 CitationsSource