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Lawrence J. DeLucas
University of Alabama at Birmingham
130Publications
24H-index
1,989Citations
Publications 130
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#2Shaunivan LabiukH-Index: 8
Last.Stephen G. AllerH-Index: 12
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We demonstrate a scalable method for the separation of the bacterial periplasm from the cytoplasm. This method is used to purify periplasmic protein for the purpose of biophysical characterization, and measure substrate transfer between periplasmic and cytoplasmic compartments. By carefully limiting the time that the periplasm is separated from the cytoplasm, the experimenter can extract the protein of interest and assay each compartment individually for substrate without carry-over contaminatio...
#2Lawrence J. DeLucasH-Index: 24
Last.Stephen G. AllerH-Index: 12
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#2Lawrence J. DeLucasH-Index: 24
Last.Stephen G. AllerH-Index: 12
view all 0 authors...
#1Christopher D. Radka (UAB: University of Alabama at Birmingham)H-Index: 1
#2Dongquan Chen (UAB: University of Alabama at Birmingham)
Last.Stephen G. Aller (UAB: University of Alabama at Birmingham)H-Index: 12
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Biological chelating molecules called siderophores are used to sequester iron and maintain its ferric state. Bacterial substrate-binding proteins (SBPs) bind iron–siderophore complexes and deliver these complexes to ATP-binding cassette (ABC) transporters for import into the cytoplasm, where the iron can be transferred from the siderophore to catalytic enzymes. In Yersinia pestis, the causative agent of plague, the Yersinia iron-uptake (Yiu) ABC transporter has been shown to improve iron acquisi...
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