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Servé W. M. Kengen
Wageningen University and Research Centre
112Publications
34H-index
3,570Citations
Publications 114
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Abstract Metabolic engineering for increased isoprenoid production often benefits from the simultaneous expression of the two naturally available isoprenoid metabolic routes, namely the 2-methyl-D-erythritol 4-phosphate (MEP) pathway and the mevalonate (MVA) pathway. Quantification of the contribution of these pathways to the overall isoprenoid production can help to obtain a better understanding of the metabolism within a microbial cell factory. Such type of investigation can benefit from 13C m...
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Rhodobacter sphaeroides is a metabolically versatile bacterium that serves as a model for analysis of photosynthesis, hydrogen production and terpene biosynthesis. The elimination of by-products formation, such as poly-β-hydroxybutyrate (PHB), has been an important metabolic engineering target for R. sphaeroides. However, the lack of efficient markerless genome editing tools for R. sphaeroides is a bottleneck for fundamental studies and biotechnological exploitation. The Cas9 RNA-guided DNA-endo...
1 CitationsSource
#1Enrico Orsi (WUR: Wageningen University and Research Centre)H-Index: 1
#2Pauline L. Folch (WUR: Wageningen University and Research Centre)H-Index: 1
Last.Ruud A. Weusthuis (WUR: Wageningen University and Research Centre)H-Index: 24
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Rhodobacter sphaeroides is a metabolically versatile bacterium capable of producing terpenes natively. Surprisingly, terpene biosynthesis in this species has always been investigated in complex media, with unknown compounds possibly acting as carbon and nitrogen sources. Here, a defined medium was adapted for R. sphaeroides dark heterotrophic growth, and was used to investigate the conversion of different organic substrates into the reporter terpene amorphadiene. The amorphadiene synthase was cl...
2 CitationsSource
#1Mamou DialloH-Index: 1
#2Rémi Hocq (French Institute of Petroleum)H-Index: 1
Last.A.M. Lopez-ContrerasH-Index: 15
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Abstract Recent developments in CRISPR technologies have opened new possibilities for improving genome editing tools dedicated to the Clostridium genus. In this study we adapted a two-plasmid tool based on this technology to enable scarless modification of the genome of two reference strains of Clostridium beijerinckii producing an Acetone/Butanol/Ethanol (ABE) or an Isopropanol/Butanol/Ethanol (IBE) mix of solvents. In the NCIMB 8052 ABE-producing strain, inactivation of the SpoIIE sporulation ...
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#1Aleksander J. Kruis (WUR: Wageningen University and Research Centre)H-Index: 2
#2Anna C. Bohnenkamp (WUR: Wageningen University and Research Centre)H-Index: 1
Last.Ruud A. Weusthuis (WUR: Wageningen University and Research Centre)H-Index: 24
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Abstract Sustainable production of bulk chemicals is one of the major challenges in the chemical industry, particularly due to their low market prices. This includes short and medium chain esters, which are used in a wide range of applications, for example fragrance compounds, solvents, lubricants or biofuels. However, these esters are produced mainly through unsustainable, energy intensive processes. Microbial conversion of biomass-derived sugars into esters may provide a sustainable alternativ...
1 CitationsSource
Last.Peter J. SchaapH-Index: 33
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The cupin-type phosphoglucose isomerase (PfPGI) from the hyperthermophilic archaeon Pyrococcus furiosus catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate. We investigated PfPGI using protein-engineering bioinformatics tools to select functionally-important residues based on correlated mutation analyses. A pair of amino acids in the periphery of PfPGI was found to be the dominant co-evolving mutation. The position of these selected residues was found to be non-...
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#1Mamou DialloH-Index: 1
Last.A.M. Lopez ContrerasH-Index: 1
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This experiment aim was to characterize the catabolism of L-rhamnose of Clostridium beijerinckii DSM 6423 by transcriptomic analysis, generating new insights and knowledge on utilization of L-rhamnose for production of chemicals, including Isopropanol, Butanol, Ethanol (IBE) and 1,2-propandiol. These analysis on cultures grown on L-rhamnose compared to D-glucose grown cultures showed upregulation of the L-rhamnose-related clusters and genes, and lower expression of the solventogenic genes, which...
#1Aleksander J. Kruis (WUR: Wageningen University and Research Centre)H-Index: 2
#2Brigida GalloneH-Index: 4
Last.Ruud A. Weusthuis (WUR: Wageningen University and Research Centre)H-Index: 24
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Esters are essential for the flavour and aroma of fermented products, and are mainly produced by alcohol acyl transferases (AATs). A recently discovered AAT family named Eat (Ethanol acetyltransferase) contributes to ethyl acetate synthesis in yeast. However, its effect on the synthesis of other esters is unknown. In this study, the role of the Eat family in ester synthesis was compared to that of other S. cerevisiae AATs (Atf1, Atf2, Eht1, and Eeb1) in silico and in vivo. A genomic study in a c...
2 CitationsSource
A prerequisite for a successful biobased economy is the efficient conversion of biomass resources into useful products, such as biofuels and bulk and specialty chemicals. In contrast to other industrial microorganisms, natural solvent-producing clostridia utilize a wide range of sugars, including C5, C6, and deoxy-sugars, for production of long-chain alcohols (butanol and 2,3-butanediol), isopropanol, acetone, n-propanol, and organic acids. Butanol production by clostridia from first-generation ...
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#1Aleksander J. Kruis (WUR: Wageningen University and Research Centre)H-Index: 2
#2Astrid E. Mars (WUR: Wageningen University and Research Centre)H-Index: 18
Last.Ruud A. Weusthuis (WUR: Wageningen University and Research Centre)H-Index: 24
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Eat1 is a recently discovered alcohol acetyltransferase responsible for bulk ethyl acetate production in yeasts such as Wickerhamomyces anomalus and Kluyveromyces lactis. These yeasts have the potential to become efficient biobased ethyl acetate producers. However, some fundamental features of Eat1 are still not understood, which hampers the rational engineering of efficient production strains. The cellular location of Eat1 in yeast is one of these features. To reveal its location, Eat1 was fuse...
3 CitationsSource
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