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Michael Fenech
Commonwealth Scientific and Industrial Research Organisation
DNA damageMicronucleusMicronucleus testGeneticsBiology
281Publications
54H-index
9,892Citations
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Publications 325
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#1Emma L JaunayH-Index: 1
Last. Michael FenechH-Index: 54
view all 6 authors...
The cytokinesis-block micronucleus cytome (CBMNcyt) assay is a comprehensive method to measure DNA damage, cytostasis and cytotoxicity caused by nutritional, radiation and chemical factors. A slide imaging technique has been identified as a new method to assist with the visual scoring of cells for the CBMNcyt assay. A NanoZoomer S60 Digital Pathology slide scanner was used to view WIL2-NS cells treated with hydrogen peroxide (H2O2) and measure CBMNcyt assay biomarkers using a high-definition des...
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#1Armen Nersesyan (Medical University of Vienna)H-Index: 23
#2Rafael MuradyanH-Index: 2
Last. Siegfried Knasmueller (Medical University of Vienna)H-Index: 21
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Abstract Introduction Smoking is an independent cause of cervical cancer, which is the 4th most common malignancy in women. It is currently not known if tobacco consumption causes chromosomal damage (which is a hallmark of human cancer) in cervical cells and if age and the hormonal status have an impact on tobacco induced genetic instability in the cervix. Methods We conducted a study with pre- and post-menopausal women smokers and never-smokers (25/group). Smokers consumed 30 light/medium cigar...
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#1Radhia M’kacherH-Index: 9
#2Bruno ColicchioH-Index: 8
Last. Noufissa Oudrhiri (Université Paris-Saclay)
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Dicentric chromosomes are a relevant marker of chromosomal instability. Their appearance is associated with telomere dysfunction, leading to cancer progression and a poor clinical outcome. Here, we present Telomere and Centromere staining followed by M-FISH (TC+M-FISH) for improved detection of telomere dysfunction and the identification of dicentric chromosomes in cancer patients and various genetic syndromes. Significant telomere length shortening and significantly higher frequencies of telome...
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#1Permal Deo (UniSA: University of South Australia)H-Index: 4
#2Varinderpal S. Dhillon (UniSA: University of South Australia)H-Index: 15
Last. Michael Fenech (UniSA: University of South Australia)H-Index: 54
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This study investigated the effect of dietary sugars and advanced glycation end-products (AGE) on telomere dynamics in WIL2-NS cells. Dietary sugars [glucose (Glu) and fructose (Fru); 0.1 M each] were incubated with bovine serum albumin (BSA) (10 mg/ml) at 60 +/- 1 degrees C for 6 weeks to generate AGE-BSA. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis showed total AGE levels as 87.74 +/- 4.46 nmol/mg and 84.94 +/- 4.28 nmol/mg respectively in Glu-BSA and Fru-BSA model. Cell treatm...
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#1Permal Deo (UniSA: University of South Australia)H-Index: 4
#2Caitlin L McCullough (UniSA: University of South Australia)H-Index: 1
Last. Michael Fenech (UniSA: University of South Australia)H-Index: 54
view all 7 authors...
This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either with glucose (Glu-BSA) or with fructose (Fru-BSA). Liquid chromatography-mass spectrometry (LC-MS/MS) analysis showed higher Nepsilon-carboxymethyllysine (CML; 26.76 +/- 1.09 nmol/mg...
4 CitationsSource
#1Michael FenechH-Index: 54
#2Nina Holland (University of California, Berkeley)H-Index: 45
Last. Stefano BonassiH-Index: 55
view all 10 authors...
Abstract The “Micronuclei and Disease” workshop was organized by the HUMN Project consortium and hosted by the European Environmental Mutagen and Genomics Society at their annual meeting in Rennes, France, on 23 May 2019. The program of the workshop focused on addressing the emerging evidence linking micronucleus (MN) frequency to human disease. The first objective was to review what has been published and evaluate the level and quality of evidence for the connection between MN frequency and var...
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#1Caroline Bull (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 4
#1Caroline Bull (University of Adelaide)H-Index: 4
Last. Michael Fenech (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 54
view all 3 authors...
Background: 5-aza-2’-deoxycytidine (5azadC, decitabine) is a DNA hypomethylating agent used in the treatment of myelodysplastic syndromes. Due to cytotoxic side effects dose optimization is essential. This study defines and quantifies the effects of 5azadC on chromosomal stability and telomere length, at clinically relevant dosages. Methods: Human WIL2-NS cells were maintained in complete medium containing 0, 0.2 or 1.0µM 5azadC for four days, and analysed daily for telomere length (flow cytomet...
1 CitationsSource
#1Permal Deo (UniSA: University of South Australia)H-Index: 4
#2Varinderpal S. Dhillon (UniSA: University of South Australia)H-Index: 15
Last. Michael Fenech (UniSA: University of South Australia)H-Index: 8
view all 5 authors...
: APOE e4 allele is an established risk factor for Alzheimer's disease and hypercholesterolemia. However, its association with metabolic and genetic risk factors related to glycation is not clear. We tested the hypothesis that, apart from high plasma cholesterol, APOE e4 carriers may also have higher advanced glycation end products (AGEs) and total soluble extracellular domain of RAGE (sRAGE) and that these biomarkers may be modified by the common Gly82Ser (G82S) polymorphism (rs2070600) in the ...
4 CitationsSource
: Apolipoprotein-e4 (APOE-e4) - common variant is a major genetic risk factor for cognitive decline and Alzheimer's disease (AD). An accelerated rate of biological aging could contribute to this increased risk. Glycation of serum proteins due to excessive glucose and reactive oxygen species leads to the formation of advanced glycation end products (AGEs) - a risk factor for diabetes and AD, and decline in motor functioning in elderly. Aim of present study was to investigate impact of APOE-e4 all...
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#2Claudia BolognesiH-Index: 42
Last. M. Fenech
view all 3 authors...
Micronucleus (MN) assays have become the most widely used and best validated biomarkers of DNA damage and chromosomal instability in human cells both in vitro and in vivo. To ensure consistent and reliable application of micronucleus assays within and across laboratories worldwide, it is essential to establish clear guidelines and detailed protocols and to test these in inter-laboratory validation exercises. This chapter focuses on (i) the process of the establishment of the approved OECD guidel...
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