Marc W. Kirschner
Harvard University
Publications 441
#1Hak-Min Kim (UNIST: Ulsan National Institute of Science and Technology)H-Index: 11
#2Jessica A. Weber (Harvard University)H-Index: 8
Last.Seungshic YumH-Index: 10
view all 23 authors...
Unique among cnidarians, jellyfish have remarkable morphological and biochemical innovations that allow them to actively hunt in the water column and were some of the first animals to become free-swimming. The class Scyphozoa, or true jellyfish, are characterized by a predominant medusa life-stage consisting of a bell and venomous tentacles used for hunting and defense, as well as using pulsed jet propulsion for mobility. Here, we present the genome of the giant Nomura’s jellyfish (Nemopilema no...
1 CitationsSource
#1Leonid Peshkin (Harvard University)H-Index: 31
#2Myriam Boukhali (Harvard University)H-Index: 12
Last.Lev Y. Yampolsky (ETSU: East Tennessee State University)H-Index: 22
view all 5 authors...
The microcrustacean Daphnia is becoming an organism of choice for genomic and proteomic studies of the effects of environmental stressors. However, the changes in protein expression across the life cycle have not been fully characterized. We analyzed the proteomes of adult females, juveniles, asexually produced embryos, and the ephippia - resting stages containing two sexually produced diapausing freezing- and desiccation-resistant embryos. Overall, we were significantly more likely to detect pr...
#1Seungeun Oh (Harvard University)H-Index: 11
#2Changhee Lee (Harvard University)H-Index: 4
Last.Marc W. Kirschner (Harvard University)H-Index: 144
view all 10 authors...
We introduce Normalized Raman Imaging (NoRI), a Stimulated Raman Scattering (SRS) microscopy method that computationally removes the effect of tissue light scattering. NoRI provides high resolution measurements of the absolute concentration of total protein, total lipid and water from live or fixed thick tissue samples with single cell resolution, and can also be applied to other Raman bands. NoRI enables study of the protein, lipid, and water concentration variation associated with development ...
1 CitationsSource
#1Wenzhe Ma (Harvard University)H-Index: 8
#2Maorong Chen (Boston Children's Hospital)
Last.Marc W. Kirschner (Harvard University)H-Index: 144
view all 7 authors...
Abstract Dvl (Dishevelled) is one of several essential non-enzymatic components of the Wnt signaling pathway. In most current models, Dvl forms complexes with Wnt ligand receptors, Fzd and LRP5/6 at the plasma membrane, which then recruits other components of the destruction complex leading to inactivation of β-catenin degradation. Although this model is widespread, direct evidence for this process is lacking. In this study, we tagged mEGFP to C-terminus of dishevlled2 gene using CRISPR/Cas9 ind...
#1Xili Liu (Harvard University)
#2Seungeun Oh (Harvard University)H-Index: 11
Last.Marc W. Kirschner (Harvard University)H-Index: 144
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The regulation of growth is fundamental to cell size control. Lack of sufficient accuracy in the measurement of the growth rate of adherent cells through the cell cycle has thwarted the understanding of how such cell populations maintain a stable size distribution. The accuracy of Quantitative Phase Microscopy (QPM) is just shy of the accuracy needed to resolve the principle features of mammalian cell growth and perhaps to reveal new ones. Based on our analysis of the source of errors in QPM we ...
#1Leonid Peshkin (Harvard University)H-Index: 31
#2Alexander A. Lukyanov (Harvard University)H-Index: 9
Last.Marc W. Kirschner (Harvard University)H-Index: 144
view all 10 authors...
Summary We present an unprecedentedly comprehensive characterization of protein dynamics across early development in Xenopus laevis, available immediately via a convenient Web portal. This resource allows interrogation of the protein expression data in conjunction with other data modalities such as genome wide mRNA expression. This study provides detailed data for absolute levels of ∼14K unique Xenopus proteins representing homologues of ∼9K unique human genes – a rich resource for developmental...
2 CitationsSource
#1Douaa Mugahid (Harvard University)
#2Marian Kalocsay (Harvard University)H-Index: 6
Last.Marc W. Kirschner (Harvard University)H-Index: 144
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The Hippo pathway, in which changes at the cell surface and in the extracellular environment control the activity of a downstream transcription factor, known as YAP in mammalian cells and Yorkie in Drosophila, has recently taken center-stage as perhaps the most important pathway in metazoans for controlling organ size. In intact tissues YAP activity is inhibited and the organ does not overgrow. When the organ is damaged, YAP is active and necessary for growth and regeneration to occur. The exact...
#1Jing Zhang (UNC: University of North Carolina at Chapel Hill)H-Index: 66
#2Tao Wu (Harvard University)H-Index: 25
Last.Qing Zhang (UNC: University of North Carolina at Chapel Hill)H-Index: 24
view all 33 authors...
Inactivation of the von Hippel-Lindau (VHL) E3 ubiquitin ligase protein is a hallmark of clear cell renal cell carcinoma (ccRCC). Identifying how pathways affected by VHL loss contribute to ccRCC remains challenging. We used a genome-wide in vitro expression strategy to identify proteins that bind VHL when hydroxylated. Zinc fingers and homeoboxes 2 (ZHX2) was found as a VHL target, and its hydroxylation allowed VHL to regulate its protein stability. Tumor cells from ccRCC patients with VHL loss...
7 CitationsSource
#1Miriam Bracha Ginzberg (Harvard University)H-Index: 8
#2Nancy ChangH-Index: 3
Last.Marc W. Kirschner (Harvard University)H-Index: 144
view all 6 authors...
Animal cells come in many different sizes. In humans, for example, egg cells are thousands of times larger than sperm cells. Yet cells of any given type are often strikingly similar in size. The cells that line the surface of organs including the skin and kidneys are especially uniform; in fact a loss of size uniformity in certain tumors is a sign of malignancy. What kind of regulation could enable separate cells within a tissue to have the same size? One possibility is that each type of cell is...
19 CitationsSource
#1James Briggs (Harvard University)H-Index: 10
#2Caleb Weinreb (Harvard University)H-Index: 10
Last.Allon M. Klein (Harvard University)H-Index: 32
view all 7 authors...
INTRODUCTION Metazoan development represents a big jump in complexity compared with unicellular life in two aspects: cell-type differentiation and cell spatial organization. In vertebrate embryos, many distinct cell types appear within just a single day of life after fertilization. Studying the developmental dynamics of all embryonic cell types is complicated by factors such as the speed of early development, complex cellular spatial organization, and scarcity of raw material for conventional an...
102 CitationsSource