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Rena Levin-Klein
University of Minnesota
Molecular biologyDNA methylationV(D)J recombinationGeneticsBiology
10Publications
5H-index
90Citations
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Publications 11
Newest
#1Emily K. LawH-Index: 11
#2Rena Levin-Klein (UMN: University of Minnesota)H-Index: 5
Last. Reuben S. HarrisH-Index: 59
view all 17 authors...
The APOBEC3 family of antiviral DNA cytosine deaminases is implicated as the second largest source of mutation in cancer. This mutational process may be a causal driver or inconsequential passenger to the overall tumor phenotype. We show that human APOBEC3A expression in murine colon and liver tissues increases tumorigenesis. All other APOBEC3 family members including APOBEC3B did not as strongly promote liver tumor formation. DNA sequences from APOBEC3A-expressing animals display hallmark APOBE...
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#1William L. BrownH-Index: 21
#2Emily K. LawH-Index: 11
Last. Reuben S. HarrisH-Index: 59
view all 11 authors...
The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B. One mAb, 5210-87-13, demonstrates utility in multiple applications, including ELISA, immunoblot, immunofluorescence microscopy, and immunohistoc...
1 CitationsSource
#1William L. Brown (UMN: University of Minnesota)H-Index: 21
#2Emily K. Law (HHMI: Howard Hughes Medical Institute)H-Index: 1
Last. Reuben S. Harris (HHMI: Howard Hughes Medical Institute)H-Index: 1
view all 11 authors...
The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B through a series of immunoblot, immunofluorescence microscopy, and immunohistochemistry experiments. Positive results indicate that one mAb, 5210...
1 CitationsSource
#1Yitzhak Reizel (UPenn: University of Pennsylvania)H-Index: 3
#2Ofra Sabag (HUJI: Hebrew University of Jerusalem)H-Index: 7
Last. Howard Cedar (HUJI: Hebrew University of Jerusalem)H-Index: 73
view all 15 authors...
Development in mammals is accompanied by specific de novo and demethylation events that are thought to stabilize differentiated cell phenotypes. We demonstrate that a large percentage of the tissue-specific methylation pattern is generated postnatally. Demethylation in the liver is observed in thousands of enhancer-like sequences associated with genes that undergo activation during the first few weeks of life. Using. conditional gene ablation strategy we show that the removal of these methyl gro...
12 CitationsSource
#1Nadine M. ShabanH-Index: 10
#2Ke Shi (UMN: University of Minnesota)H-Index: 16
Last. Reuben S. HarrisH-Index: 59
view all 13 authors...
Summary Human APOBEC3H and homologous single-stranded DNA cytosine deaminases are unique to mammals. These DNA-editing enzymes function in innate immunity by restricting the replication of viruses and transposons. APOBEC3H also contributes to cancer mutagenesis. Here, we address the fundamental nature of RNA in regulating human APOBEC3H activities. APOBEC3H co-purifies with RNA as an inactive protein, and RNase A treatment enables strong DNA deaminase activity. RNA-binding-defective mutants demo...
16 CitationsSource
#1Rena Levin-KleinH-Index: 5
#2Shira FraenkelH-Index: 4
Last. Yehudit BergmanH-Index: 36
view all 10 authors...
B cell development involves sequential rearrangement of the immunoglobulin chains, but fine control over the selection process remains a mystery. Here the authors show that individual alleles in pre-B cells are clonally unique and result from stochastic activation of V gene segments to induce optimal generation of a diverse repertoire.
5 CitationsSource
#1Shari Orlanski (HUJI: Hebrew University of Jerusalem)H-Index: 6
#2Verena LabiH-Index: 21
Last. Yehudit Bergman (HUJI: Hebrew University of Jerusalem)H-Index: 36
view all 11 authors...
There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the e...
36 CitationsSource
#1Rena Levin-KleinH-Index: 5
#2Yehudit BergmanH-Index: 36
The immunoglobulin light chain loci (Igκ and Igλ) are recombined via V(D)J recombination at the pre-B cell stage during B cell differentiation. The recombination process is highly regulated on multiple levels to ensure proper timing and locus specificity. In this article, we focus on a number of mechanisms which contribute to the tight control of the rearrangement, focusing on the Igκ locus, which is the primary IgL chain utilized in both mice and humans. We explain how regulation of locus acces...
1 CitationsSource
#1Rena Levin-Klein (HUJI: Hebrew University of Jerusalem)H-Index: 5
#2Yehudit Bergman (HUJI: Hebrew University of Jerusalem)H-Index: 36
While most genes in the mammalian genome are transcribed from both parental chromosomes in cells where they are expressed, approximately 10% of genes are expressed monoallelically, so that any given cell will express either the paternal or maternal allele, but not both. The antigen receptor genes in B and T cells are well studied examples of a gene family which is expressed in a monoallelic manner, in a process coined "allelic exclusion". During lymphocyte development, only one allele of each an...
14 CitationsSource
#1Rena Levin-Klein (HUJI: Hebrew University of Jerusalem)H-Index: 5
#2Andrei Kirillov (HUJI: Hebrew University of Jerusalem)H-Index: 5
Last. Yehudit Bergman (HUJI: Hebrew University of Jerusalem)H-Index: 36
view all 5 authors...
The Igκ locus undergoes a variety of different molecular processes during B cell development, including V(D)J rearrangement and somatic hypermutations, which are influenced by cis regulatory regions (RR) within the locus. The Igκ locus includes three characterized RRs termed the intronic (iEκ), 3’Eκ and Ed enhancers. We had previously noted that a region of DNA upstream of the iEκ and matrix attachment region (MAR) was necessary for demethylation of the locus in cell culture. In this study, we f...
5 CitationsSource
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