Stanislav Y. Shvartsman
Princeton University
EmbryoGeneticsMAPK/ERK pathwayBiologyCell biology
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Publications 104
#1Celia M. SmitsH-Index: 1
#1Aleena L. Patel (Princeton University)H-Index: 1
#2Eyan Yeung (Princeton University)H-Index: 3
Last. Stanislav Y. Shvartsman (Princeton University)H-Index: 34
view all 7 authors...
Optogenetic approaches are transforming quantitative studies of cell-signaling systems. A recently developed photoswitchable mitogen-activated protein kinase kinase 1 (MEK1) enzyme (psMEK) short-circuits the highly conserved Extracellular Signal-Regulated Kinase (ERK)-signaling cascade at the most proximal step of effector kinase activation. However, since this optogenetic tool relies on phosphorylation-mimicking substitutions in the activation loop of MEK, its catalytic activity is predicted to...
#1Sayantan Dutta (Princeton University)
#2Nareg J.-V. Djabrayan (Princeton University)H-Index: 1
Last. Matej Krajnc (Princeton University)H-Index: 5
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Abstract Embryonic development starts with cleavages, a rapid sequence of reductive divisions that result in an exponential increase of cell number without changing the overall size of the embryo. In Drosophila, the final four rounds of cleavages occur at the surface of the embryo and give rise to ∼6000 nuclei under a common plasma membrane. We use live imaging to study the dynamics of this process and to characterize the emergent nuclear packing in this system. We show that the characteristic l...
#1Clinton A. Taylor (UTSW: University of Texas Southwestern Medical Center)H-Index: 3
#2Kevin W. Cormier (UTSW: University of Texas Southwestern Medical Center)H-Index: 5
Last. Melanie H. Cobb (UTSW: University of Texas Southwestern Medical Center)H-Index: 38
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The most frequent extracellular signal-regulated kinase 2 (ERK2) mutation occurring in cancers is E322K (E-K). ERK2 E-K reverses a buried charge in the ERK2 common docking (CD) site, a region that binds activators, inhibitors, and substrates. Little is known about the cellular consequences associated with this mutation, other than apparent increases in tumor resistance to pathway inhibitors. ERK2 E-K, like the mutation of the preceding aspartate (ERK2 D321N [D-N]) known as the sevenmaker mutatio...
#1Yonghyun Song (Princeton University)H-Index: 1
#2Junyoung O. Park (UCLA: University of California, Los Angeles)H-Index: 10
Last. Stanislav Y. Shvartsman (Princeton University)H-Index: 22
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Summary Eggs of oviparous animals must be prepared to develop rapidly and robustly until hatching. The balance between sugars, fats, and other macromolecules must therefore be carefully considered when loading the egg with nutrients. Clearly, packing too much or too little fuel would lead to suboptimal conditions for development. While many studies have measured the overall energy utilization of embryos, little is known of the identity of the molecular-level processes that contribute to the ener...
1 CitationsSource
#1Stanislav Y. Shvartsman (Princeton University)H-Index: 22
#2Matej Krajnc (Princeton University)H-Index: 5
Deneke et al. (2019) discover that dynamic interactions of cell cycle and actomyosin contractility systems synchronize nuclear cleavages, generating a cytoplasmic flow that results in a spatially uniform distribution of zygotic nuclei in the early Drosophila embryo. This work underscores the importance of self-organizing mechanisms before the onset of zygotic transcription.
#1Nareg J.-V. Djabrayan (Princeton University)H-Index: 1
#2Celia M. Smits (Princeton University)H-Index: 1
Last. Stanislav Y. Shvartsman (Princeton University)H-Index: 22
view all 9 authors...
Summary The thirteen nuclear cleavages that give rise to the Drosophila blastoderm are some of the fastest known cell cycles [ 1 ]. Surprisingly, the fertilized egg is provided with at most one-third of the dNTPs needed to complete the thirteen rounds of DNA replication [ 2 ]. The rest must be synthesized by the embryo, concurrent with cleavage divisions. What is the reason for the limited supply of DNA building blocks? We propose that frugal control of dNTP synthesis contributes to the well-cha...
7 CitationsSource
#1Katherine A. Rauen (UC Davis: University of California, Davis)H-Index: 34
#2Lisa SchoyerH-Index: 4
Last. Frank McCormick (UCSF: University of California, San Francisco)H-Index: 115
view all 37 authors...
#1Jasmin Imran Alsous (Princeton University)H-Index: 3
#2Paul Villoutreix (Princeton University)H-Index: 3
Last. Jörn Dunkel (MIT: Massachusetts Institute of Technology)H-Index: 25
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Optimal packings1,2 of unconnected objects have been studied for centuries3–6, but the packing principles of linked objects, such as topologically complex polymers7,8 or cell lineages9,10, are yet to be fully explored. Here, we identify and investigate a generic class of geometrically frustrated tree packing problems, arising during the initial stages of animal development when interconnected cells assemble within a convex enclosure10. Using a combination of 3D imaging, computational image analy...
3 CitationsSource
#1Yogesh Goyal (Princeton University)H-Index: 10
#2Trudi Schüpbach (Princeton University)H-Index: 31
Last. Stanislav Y. Shvartsman (Princeton University)H-Index: 22
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Abstract Receptor tyrosine kinases (RTKs) control a wide range of developmental processes, from the first stages of embryogenesis to postnatal growth and neurocognitive development in the adult. A significant share of our knowledge about RTKs comes from genetic screens in model organisms, which provided numerous examples demonstrating how specific cell fates and morphologies are abolished when RTK activation is either abrogated or significantly reduced. Aberrant activation of such pathways has a...
2 CitationsSource