Marianthe Hamilton-Wessler
University of Southern California
Publications 15
#1Martin EllmererH-Index: 3
Last.Richard N. BergmanH-Index: 96
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Physiological hyperinsulinemia provokes hemodynamic actions and augments access of macromolecules to insulin-sensitive tissues. We investigated whether induction of insulin resistance by a hypercaloric high-fat diet has an effect on the extracellular distribution of macromolecules to insulin-sensitive tissues. Male mongrel dogs were randomly selected into two groups: seven dogs were fed an isocaloric control diet (∼3,900 kcal, 35% from fat), and six dogs were fed a hypercaloric high-fat diet (∼5...
37 CitationsSource
#1Martin EllmererH-Index: 19
#2Stella P. KimH-Index: 18
Last.Richard N. BergmanH-Index: 96
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Pharmacological doses of insulin increase limb blood flow and enhance tissue recruitment for small solutes such as glucose. We investigated whether elevating insulin within the physiological range (68 ± 6 vs. 425 ± 27 pmol/l) can influence tissue recruitment of [ 14 C]inulin, an inert diffusionary marker of molecular weight similar to that of insulin itself. During hyperinsulinemic-euglycemic clamps, transport parameters and distribution volumes of [ 14 C]inulin were determined in conscious dogs...
13 CitationsSource
#1Martin Ellmerer (SC: University of Southern California)H-Index: 3
#2Marianthe Hamilton-Wessler (SC: University of Southern California)H-Index: 11
Last.Richard N. Bergman (SC: University of Southern California)H-Index: 96
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We compared metabolic effects as well as plasma and interstital fluid kinetics of fatty acid-acylated insulin, LysB29(Ne-ω-carboxynonadecanoyl)-des(B30) human insulin (O346), with previously determined kinetics of native insulin and insulin detemir. Euglycemic clamps with iv injection of O346 (90 pmol/kg) or saline control were performed in 10 male mongrel dogs under inhalent anesthesia. The t1/2 for the clearance of O346 from plasma was 375.7 ± 26.7 min; the t1/2 for the appearance of O346 in i...
3 CitationsSource
Rapid oscillations of visceral lipolysis have been reported. To examine the putative role of the CNS in oscillatory lipolysis, we tested the effects of β3-blockade on pulsatile release of FFAs. Arterial blood samples were drawn at 1-minute intervals for 120 minutes from fasted, conscious dogs (n = 7) during the infusion of saline or bupranolol (1.5 μg/kg/min), a high-affinity β3-blocker. FFA and glycerol time series were analyzed and deconvolution analysis was applied to estimate the rate of FFA...
63 CitationsSource
#1Melvin K. DeaH-Index: 9
Last.Richard N. BergmanH-Index: 96
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NN304 [Lys B29 -tetradecanoyl des(B30) human insulin] is a potentially therapeutic insulin analog designed to exhibit protracted glucose-lowering action. In dogs with infusion rates similar to insulin itself, NN304 exhibits similar glucose uptake ( R d ) stimulation with delayed onset of action. This compartmental modeling study was to determine if NN304 action could be accounted for by the ∼2% unbound NN304 concentration. NN304 (or human insulin) ( n = 6 each) was infused at 10.2 pmol · min −1 ...
67 CitationsSource
#2Marilyn AderH-Index: 28
Last.Richard N. BergmanH-Index: 96
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A defect in transcapillary transport of insulin in skeletal muscle and adipose tissue has been proposed to play a role in the insulin resistance that leads to type 2 diabetes, yet the mechanism of insulin transfer across the capillary endothelium from plasma to interstitium continues to be debated. This study examined in vivo the interstitial appearance of insulin in hindlimb using the fatty acid acylated insulin analog Lys B29 -tetradecanoyl des-(B30) human insulin, or NN304, as a marker for in...
43 CitationsSource
#1Richard N. Bergman (SC: University of Southern California)H-Index: 96
#2Gregg W. Van Citters (SC: University of Southern California)H-Index: 8
Last.Martin Ellmerer (SC: University of Southern California)H-Index: 3
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Abstract Insulin resistance is associated with a plethora of chronic illnesses, including Type 2 diabetes, dyslipidemia, clotting dysfunction, and colon cancer. The relationship between obesity and insulin resistance is well established, and an increase in obesity in Western countries is implicated in increased incidence of diabetes and other diseases. Central, or visceral, adiposity has been particularly associated with insulin resistance; however, the mechanisms responsible for this associatio...
178 CitationsSource
#1Steven D. MittelmanH-Index: 30
#2G W Van CittersH-Index: 2
Last.Richard N. BergmanH-Index: 96
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Central adiposity is highly correlated with insulin resistance, which is an important risk factor for type 2 diabetes and other chronic diseases. However, in normal individuals, central adiposity can be tolerated for many years without development of impaired glucose tolerance or diabetes. Here we examine longitudinally the mechanisms by which glucose tolerance can be maintained in the face of substantial insulin resistance. Normal dogs were fed a diet enriched with moderate amounts of fat (2 g ...
168 CitationsSource
#1Marianthe Hamilton-Wessler (SC: University of Southern California)H-Index: 11
#2Marilyn Ader (SC: University of Southern California)H-Index: 28
Last.Richard N. Bergman (SC: University of Southern California)H-Index: 96
view all 7 authors...
Aims/hypothesis. The provision of stable, reproducible basal insulin is crucial to diabetes management. This study in dogs examined the metabolic effects and interstitial fluid (ISF) profiles of fatty acid acylated insulin, LysB29-tetradecanoyl, des-(B30) human insulin (NN304). Methods. Euglycaemic clamps were carried out under inhalant anaesthesia during equimolar intravenous infusions (3.6 pmol · min–1· kg–1 for 480 min) of human insulin or NN304 (n = 8 per group). Results. Steady-state total ...
70 CitationsSource
#1Arne Melander (Malmö University)H-Index: 51
#2J. Olsson (Malmö University)H-Index: 1
Last.M. Cummings (Western General Hospital)H-Index: 2
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6 CitationsSource