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Francesca Tasca
Leiden University Medical Center
Gene targetingGenomeGenome editingPseudogeneTrans-actingDNABiologyCRISPR
2Publications
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#1Francesca TascaH-Index: 1
#2Qian WangH-Index: 1
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Gene editing permits changing specific DNA sequences within the vast genomes of human cells. Stem cells are particularly attractive targets for gene editing interventions as their self-renewal and differentiation capabilities consent studying cellular differentiation processes, screening small-molecule drugs, modeling human disorders, and testing regenerative medicines. To integrate gene editing and stem cell technologies, there is a critical need for achieving efficient delivery of the necessar...
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#1Xiaoyu Chen (LUMC: Leiden University Medical Center)H-Index: 7
#2Francesca Tasca (LUMC: Leiden University Medical Center)H-Index: 1
Last. Manuel A. F. V. Gonçalves (LUMC: Leiden University Medical Center)H-Index: 23
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Genome editing typically involves recombination between donor nucleic acids and acceptor genomic sequences subjected to double-stranded DNA breaks (DSBs) made by programmable nucleases (e.g. CRISPR-Cas9). Yet, nucleases yield off-target mutations and, most pervasively, unpredictable target allele disruptions. Remarkably, to date, the untoward phenotypic consequences of disrupting allelic and non-allelic (e.g. pseudogene) sequences have received scant scrutiny and, crucially, remain to be address...
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