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Yage Zhang
Ministry of Education
Conditional gene knockoutAlleleZebrafishBiologyCRISPR
3Publications
1H-index
3Citations
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Publications 3
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#1Bingzhou Han (Ministry of Education)H-Index: 1
#2Yage Zhang (Ministry of Education)H-Index: 1
Last. Bo Zhang (Ministry of Education)H-Index: 3
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Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function. However, contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been reported. Here we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NHEJ-mediated unidirectional targeted i...
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#1Wenyuan Li (Ministry of Education)H-Index: 1
#1Wenyuan Li (PKU: Peking University)H-Index: 1
Last. Bo Zhang (PKU: Peking University)H-Index: 36
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CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining conditional knockout (CKO) with fluorescent gene-labeling or further allele-labeling has been reported. Here, we developed a dual-cassette-donor strategy and achieved one-step and efficient generatio...
3 CitationsSource
#1Wenyuan Li (Ministry of Education)H-Index: 1
#2Yage Zhang (Ministry of Education)H-Index: 1
Last. Bo Zhang (Ministry of Education)H-Index: 3
view all 13 authors...
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