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Andreas Buchbender
ICLIPRNA-binding proteinRNA splicingRNAComputational biology
2Publications
1H-index
2Citations
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#1Andreas BuchbenderH-Index: 1
#2Holger MutterH-Index: 1
Last. Julian KönigH-Index: 20
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Abstract Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) is a state-of-the-art technology to map the RNA interaction sites of an RNA-binding protein (RBP) across the transcriptome. Here, we present the new iCLIP2 protocol that allows to obtain high-quality iCLIP libraries in a fast and efficient manner. The new protocol comprises separate adapter ligations, two cDNA amplification steps and bead-based size selection. The full procedure can be completed within four...
2 CitationsSource
#1Hyun-Seo Kang (TUM: Technische Universität München)H-Index: 4
#2Carolina Sánchez-Rico (TUM: Technische Universität München)H-Index: 2
Last. Andreas BuchbenderH-Index: 1
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The recognition of cis-regulatory RNA motifs in human transcripts by RNA binding proteins (RBPs) is essential for gene regulation. The molecular features that determine RBP specificity are often poorly understood. Here, we combined NMR structural biology with high-throughput iCLIP approaches to identify a regulatory mechanism for U2AF2 RNA recognition. We found that the intrinsically disordered linker region connecting the two RNA recognition motif (RRM) domains of U2AF2 mediates autoinhibitory ...
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