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Yang Liu
Peking University
39Publications
10H-index
392Citations
Publications 10
Newest
#1Jianhang Yin (PKU: Peking University)H-Index: 1
#2Mengzhu Liu (PKU: Peking University)H-Index: 1
Last.Jiazhi Hu (PKU: Peking University)H-Index: 12
view all 9 authors...
Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to chromosomal translocations and large deletions by PEM-seq. ...
1 CitationsSource
#1Xiong Xiao (PKU: Peking University)
#2Weifeng Lai (PKU: Peking University)H-Index: 3
Last.HongkuiDeng (PKU: Peking University)H-Index: 52
view all 15 authors...
The limited number of human hematopoietic stem cells (HSCs) has restrained their widespread clinical application. Despite great efforts in recent years, the in vitro expansion of HSCs remains a challenge due to incomplete understanding of the signaling networks underlying HSC self-renewal. Here, we show that culturing human cord blood (CB) CD34+ cells with JNK-IN-8, an inhibitor of the JNK signaling pathway, can enhance the self-renewal of HSCs with a 3.88-fold increase in cell number. These cul...
Source
#1Zhuangzhuang Fan (PKU: Peking University)
#2Shichen Sun (PKU: Peking University)H-Index: 2
Last.Hailan Feng (PKU: Peking University)H-Index: 11
view all 9 authors...
Source
#1Yang Liu (PKU: Peking University)H-Index: 10
#2Yang Liu (PKU: Peking University)H-Index: 57
Last.Zengyichang (PKU: Peking University)H-Index: 27
view all 6 authors...
ATP synthase, a highly conserved multi-subunit enzyme complex having a common stoichiometry of α3β3γδϵab2c8-15, functions to supply ATP as the universal energy currency for cells. It comprises of the peripheral F1 sector (α3β3γδϵ) and the membrane-integrated Fo sector (ab2c8-15). In vitro structural analyses revealed that the C-terminal domain of the ϵ-subunit could adopt either an "inserted" or "non-inserted" state (with or without interacting with the α/β-subunits), with the former being viewe...
Source
#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last.Jiazhi HuH-Index: 12
view all 4 authors...
Source
#1Jianhang YinH-Index: 1
#2Mengzhu LiuH-Index: 1
Last.Jiazhi HuH-Index: 12
view all 4 authors...
Source
#1Fenghui Guan (ZJU: Zhejiang University)H-Index: 1
#2Jiayu Yu (PKU: Peking University)H-Index: 3
Last.Sheng Ye (ZJU: Zhejiang University)H-Index: 2
view all 9 authors...
1 CitationsSource
#1LiHong Wu (PKU: Peking University)H-Index: 1
#2Yang Liu (PKU: Peking University)H-Index: 10
Last.Daochun Kong (PKU: Peking University)H-Index: 13
view all 3 authors...
Chromosomal DNA replication is one of the central biological events occurring inside cells. Due to its large size, the replication of genomic DNA in eukaryotes initiates at hundreds to tens of thousands of sites called DNA origins so that the replication could be completed in a limited time. Further, eukaryotic DNA replication is sophisticatedly regulated, and this regulation guarantees that each origin fires once per S phase and each segment of DNA gets duplication also once per cell cycle. The...
7 CitationsSource
#1Jiazhi Hu (PKU: Peking University)H-Index: 12
#2Lei Sun (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Daochun Kong (PKU: Peking University)H-Index: 13
view all 15 authors...
SUMMARY When replication forks stall at damaged bases or upon nucleotide depletion, the intra-S phase checkpoint ensures they are stabilized and can restart. In intra-S checkpoint-deficient budding yeast, stalling forks collapse, and � 10% form pathogenic chicken foot structures, contributing to incomplete replication and cell death (Lopes et al., 2001; Sogo et al., 2002; Tercero and Diffley, 2001). Using fission yeast, we report that the Cds1 Chk2 effector kinase targets Dna2 on S220 to regulat...
100 CitationsSource
#1Chengyan Wang (PKU: Peking University)H-Index: 8
#2Xuming Tang (PKU: Peking University)H-Index: 1
Last.HongkuiDeng (PKU: Peking University)H-Index: 52
view all 14 authors...
Embryonic hematopoiesis is a complex process. Elucidating the mechanism regulating hematopoietic differentiation from pluripotent stem cells would allow us to establish a strategy to efficiently generate hematopoietic cells. However, the mechanism governing the generation of hematopoietic progenitors from human embryonic stem cells (hESCs) remains unknown. Here, on the basis of the emergence of CD43+ hematopoietic cells from hemogenic endothelial (HE) cells, we demonstrated that VEGF was essenti...
46 CitationsSource
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