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Chulan Kwon
University of California, San Francisco
5Publications
Publications 5
Newest
Published on 2019in Circulation Research15.86
Suraj Kannan2
Estimated H-index: 2
(JHUSOM: Johns Hopkins University School of Medicine),
Matthew Miyamoto1
Estimated H-index: 1
(JHUSOM: Johns Hopkins University School of Medicine)
+ 5 AuthorsChulan Kwon (JHUSOM: Johns Hopkins University School of Medicine)
Published on Jul 3, 2019in Journal of Visualized Experiments1.11
Emmanouil Tampakakis9
Estimated H-index: 9
(Johns Hopkins University),
Matthew Miyamoto1
Estimated H-index: 1
(Johns Hopkins University),
Chulan Kwon16
Estimated H-index: 16
(Johns Hopkins University)
Pluripotent stem cells offer great potential for understanding heart development and disease and for regenerative medicine. While recent advances in developmental cardiology have led to generating cardiac cells from pluripotent stem cells, it is unclear if the two cardiac fields - the first and second heart fields (FHF and SHF) — are induced in pluripotent stem cells systems. To address this, we generated a protocol for in vitro specification and isolation of heart field-specific cardiac progeni...
Published on Nov 6, 2018in Circulation23.05
Emmanouil Tampakakis9
Estimated H-index: 9
,
Amir Saberi4
Estimated H-index: 4
+ 1 AuthorsChulan Kwon
Introduction: Sympathetic neurons (SNs) regulate heart rate, conduction velocity, contractility and relaxation of the myocardium. Disruption of SNs in adult cardiac disease, leads to arrhythmias, myocardial dysfunction and sudden cardiac death. However, the role of SNs during cardiac development and
Published on Nov 14, 2017in Circulation23.05
Peter Andersen11
Estimated H-index: 11
,
Dennisse Jimenez-Cyrus + 6 AuthorsChulan Kwon
Over the past few decades, major advances have been made in identifying the origins of cardiac cells from developing embryos. In particular, the discovery of the first heart field (FHF) and the sec...
Published on Oct 1, 2017in Nature Protocols11.33
Gun Sik Cho3
Estimated H-index: 3
,
Emmanouil Tampakakis9
Estimated H-index: 9
+ 1 AuthorsChulan Kwon16
Estimated H-index: 16
This protocol describes how to generate mature adult-like cardiomyocytes by culturing mouse or human PSCs in vitro initially and then transferring to neonatal rats for further cell maturation.
Published on Nov 11, 2016in Circulation23.05
Emmanouil Tampakakis9
Estimated H-index: 9
(Johns Hopkins University),
Peter Andersen11
Estimated H-index: 11
(Johns Hopkins University)
+ 2 AuthorsChulan Kwon16
Estimated H-index: 16
(Johns Hopkins University)
Introduction: Congenital heart disease is the most common birth defect (1% of live born infants) and most children, who survive to adulthood, will develop severe heart failure. At present, heart transplantation is the only definite treatment for advanced heart failure patients. Heart development involves an early assignment of two distinct cellular populations, called cardiac progenitor cells (CPCs), which generate the first and the second heart field and subsequently the left and right ventricu...
Published on Jul 1, 2016in Cell Stem Cell21.46
Yohan Oh3
Estimated H-index: 3
(JHUSOM: Johns Hopkins University School of Medicine),
Gun Sik Cho1
Estimated H-index: 1
(JHUSOM: Johns Hopkins University School of Medicine)
+ 10 AuthorsRichard L. Huganir121
Estimated H-index: 121
(JHUSOM: Johns Hopkins University School of Medicine)
Summary Neurons derived from human pluripotent stem cells (hPSCs) are powerful tools for studying human neural development and diseases. Robust functional coupling of hPSC-derived neurons with target tissues in vitro is essential for modeling intercellular physiology in a dish and to further translational studies, but it has proven difficult to achieve. Here, we derive sympathetic neurons from hPSCs and show that they can form physical and functional connections with cardiac muscle cells. Using ...
Published on Nov 1, 2015in Cell Reports7.82
Hideki Uosaki14
Estimated H-index: 14
(Johns Hopkins University),
Patrick Cahan23
Estimated H-index: 23
(Harvard University)
+ 5 AuthorsChulan Kwon16
Estimated H-index: 16
(Johns Hopkins University)
Summary Decades of progress in developmental cardiology has advanced our understanding of the early aspects of heart development, including cardiomyocyte (CM) differentiation. However, control of the CM maturation that is subsequently required to generate adult myocytes remains elusive. Here, we analyzed over 200 microarray datasets from early embryonic to adult hearts and identified a large number of genes whose expression shifts gradually and continuously during maturation. We generated an atl...
Published on Apr 24, 2014in eLife7.55
Lincoln Shenje5
Estimated H-index: 5
(Johns Hopkins University),
Peter Andersen11
Estimated H-index: 11
(Johns Hopkins University)
+ 8 AuthorsDavid A. Kass116
Estimated H-index: 116
(Johns Hopkins University)
Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development, yet the intrinsic factors and environment governing these processes remain unclear. Here, we show that deletion of the ancient cell-fate regulator Numb (Nb) and its homologue Numblike (Nbl) depletes CPCs in second pharyngeal arches (PA2s) and is associated with an atrophic heart. With histological, flow cytometric and functional analyses, we find that CPCs remain undifferentia...
12
Co-Authors