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Wei Li
Chinese Academy of Sciences
91Publications
41H-index
8,855Citations
Publications 91
Newest
#1Fei Teng (CAS: Chinese Academy of Sciences)H-Index: 8
#2Jing Li (CAS: Chinese Academy of Sciences)H-Index: 2
Last.Dali Han (CAS: Chinese Academy of Sciences)H-Index: 1
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CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness six novel Cas12a orthologs for genome editing in human and mouse cells, some of which utilize simple protospacer adjacent motifs (PAMs) that remarkably increase the targeting range in the genomes. Mor...
#1Muhammad Babar Khawar (CAS: Chinese Academy of Sciences)
#2Hui Gao (CAS: Chinese Academy of Sciences)
Last.Wei Li (CAS: Chinese Academy of Sciences)H-Index: 41
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During sexual reproduction, two haploid gametes fuse to form the zygote, and the acrosome is essential to this fusion process (fertilization) in animals. The acrosome is a special kind of organelle with a cap-like structure that covers the anterior portion of the head of a the spermatozoon. The acrosome is derived from the Golgi apparatus and contains digestive enzymes. With the progress of our understanding underlying of acrosome biogenesis, a number of models have been proposed to address the ...
#1Hui Gao (CAS: Chinese Academy of Sciences)
#2Muhammad Babar Khawar (CAS: Chinese Academy of Sciences)
Last.Wei Li (CAS: Chinese Academy of Sciences)H-Index: 41
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ABSTRACTSexual reproduction is the most common form of reproduction among eukaryotes, which is characterized by a series of massive cellular or tissue renovations. Recent studies have revealed nove...
#1Li Y (CAS: Chinese Academy of Sciences)H-Index: 10
#2Leyun Wang (CAS: Chinese Academy of Sciences)H-Index: 5
Last.Jiabao Han (CAS: Chinese Academy of Sciences)H-Index: 1
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Meiosis with a single round of DNA replication and two successive rounds of chromosome segregation requires specific cyclins associated with cyclin-dependent kinases (CDKs) to ensure its fidelity. But how cyclins control the distinctive meiosis is still largely unknown. In this study, we explored the role of cyclin B3 in female meiosis by generating Ccnb3 mutant mice via CRISPR/Cas9. Ccnb3 mutant oocytes characteristically arrested at metaphase I (MetI) with normal spindle assembly and lacked en...
#1Jiaqiang WangH-Index: 13
#2Leyun Wang (CAS: Chinese Academy of Sciences)H-Index: 5
Last.Junchao Shi (University of Nevada, Reno School of Medicine)H-Index: 9
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Summary In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET , is transiently and asymmetrically expressed in the nucleus of two- to four-cell mouse embryos. Overexpression of LincGET in one of the two-cell blastomeres biases its progeny predominantly toward the inner cell mass (ICM) fate. Mechanistically, LincGET physically binds to CARM1 and promotes the ...
#1Fei Teng (CAS: Chinese Academy of Sciences)H-Index: 8
#2Tongtong Cui (CAS: Chinese Academy of Sciences)H-Index: 3
Last.Wei Li (CAS: Chinese Academy of Sciences)H-Index: 41
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The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently, CRISPR-Cas12b/C2c1, a distinct type V-B system, has been characterized as a dual-RNA-guided DNA endonuclease system. Though being active in vitro, its cleavage activity at endogenous genome remains to be explored. Furthermore, the optimal cleavage temperature of the reported Cas12b orthologs is higher than 40 °C, which is unsuitable ...
#1Zhikun Li (CAS: Chinese Academy of Sciences)H-Index: 5
#2Leyun Wang (CAS: Chinese Academy of Sciences)H-Index: 5
Last.Ying Zhang (CAS: Chinese Academy of Sciences)H-Index: 25
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Summary Unisexual reproduction is widespread among lower vertebrates, but not in mammals. Deletion of the H19 imprinted region in immature oocytes produced bimaternal mice with defective growth; however, bipaternal reproduction has not been previously achieved in mammals. We found that cultured parthenogenetic and androgenetic haploid embryonic stem cells (haESCs) display DNA hypomethylation resembling that of primordial germ cells. Through MII oocyte injection or sperm coinjection with hypometh...
#1Weiqi ZhangH-Index: 17
#2Haifeng Wan (CAS: Chinese Academy of Sciences)H-Index: 11
Last.Zhiguo Chen (Capital Medical University)H-Index: 11
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SIRT6 acts as a longevity protein in rodents1,2. However, its biological function in primates remains largely unknown. Here we generate a SIRT6-null cynomolgus monkey (Macaca fascicularis) model using a CRISPR–Cas9-based approach. SIRT6-deficient monkeys die hours after birth and exhibit severe prenatal developmental retardation. SIRT6 loss delays neuronal differentiation by transcriptionally activating the long non-coding RNA H19 (a developmental repressor), and we were able to recapitulate thi...
#1Xuepeng WangH-Index: 5
#2Tianda LiH-Index: 9
Last.Xinxin ZhangH-Index: 5
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Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis
#1Wei Li (CAS: Chinese Academy of Sciences)H-Index: 41
#2Qi Zhou (CAS: Chinese Academy of Sciences)H-Index: 51
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