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Wei Li
Chinese Academy of Sciences
91Publications
41H-index
8,846Citations
Publications 91
Newest
Published on Feb 5, 2019in Genome Biology 13.21
Fei Teng8
Estimated H-index: 8
(Chinese Academy of Sciences),
Jing Li2
Estimated H-index: 2
(Chinese Academy of Sciences)
+ 8 AuthorsQi Zhou51
Estimated H-index: 51
(Chinese Academy of Sciences)
CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness six novel Cas12a orthologs for genome editing in human and mouse cells, some of which utilize simple protospacer adjacent motifs (PAMs) that remarkably increase the targeting range in the genomes. Mor...
1 Citations Source Cite
Published on May 6, 2019in Journal of Cell Biology 8.78
Li Y10
Estimated H-index: 10
(Chinese Academy of Sciences),
Leyun Wang5
Estimated H-index: 5
(Chinese Academy of Sciences)
+ 14 AuthorsJiabao Han1
Estimated H-index: 1
(Chinese Academy of Sciences)
Meiosis with a single round of DNA replication and two successive rounds of chromosome segregation requires specific cyclins associated with cyclin-dependent kinases (CDKs) to ensure its fidelity. But how cyclins control the distinctive meiosis is still largely unknown. In this study, we explored the role of cyclin B3 in female meiosis by generating Ccnb3 mutant mice via CRISPR/Cas9. Ccnb3 mutant oocytes characteristically arrested at metaphase I (MetI) with normal spindle assembly and lacked en...
2 Citations Source Cite
Published on Dec 1, 2018in Cell 31.40
Jiaqiang Wang13
Estimated H-index: 13
,
Leyun Wang5
Estimated H-index: 5
(Chinese Academy of Sciences)
+ 12 AuthorsJunchao Shi9
Estimated H-index: 9
(University of Nevada, Reno School of Medicine)
Summary In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET , is transiently and asymmetrically expressed in the nucleus of two- to four-cell mouse embryos. Overexpression of LincGET in one of the two-cell blastomeres biases its progeny predominantly toward the inner cell mass (ICM) fate. Mechanistically, LincGET physically binds to CARM1 and promotes the ...
3 Citations Source Cite
Published on Dec 1, 2018in Cell discovery
Fei Teng8
Estimated H-index: 8
(Chinese Academy of Sciences),
Tongtong Cui3
Estimated H-index: 3
(Chinese Academy of Sciences)
+ 7 AuthorsWei Li41
Estimated H-index: 41
(Chinese Academy of Sciences)
The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently, CRISPR-Cas12b/C2c1, a distinct type V-B system, has been characterized as a dual-RNA-guided DNA endonuclease system. Though being active in vitro, its cleavage activity at endogenous genome remains to be explored. Furthermore, the optimal cleavage temperature of the reported Cas12b orthologs is higher than 40 °C, which is unsuitable ...
4 Citations Source Cite
Published on Nov 1, 2018in Cell Stem Cell 23.29
Zhikun Li5
Estimated H-index: 5
(Chinese Academy of Sciences),
Leyun Wang5
Estimated H-index: 5
(Chinese Academy of Sciences)
+ 12 AuthorsYing Zhang25
Estimated H-index: 25
(Chinese Academy of Sciences)
Summary Unisexual reproduction is widespread among lower vertebrates, but not in mammals. Deletion of the H19 imprinted region in immature oocytes produced bimaternal mice with defective growth; however, bipaternal reproduction has not been previously achieved in mammals. We found that cultured parthenogenetic and androgenetic haploid embryonic stem cells (haESCs) display DNA hypomethylation resembling that of primordial germ cells. Through MII oocyte injection or sperm coinjection with hypometh...
5 Citations Source Cite
Published on Aug 1, 2018in Nature 41.58
Weiqi Zhang17
Estimated H-index: 17
,
Haifeng Wan11
Estimated H-index: 11
(Chinese Academy of Sciences)
+ 15 AuthorsZhiguo Chen11
Estimated H-index: 11
(Capital Medical University)
SIRT6 acts as a longevity protein in rodents1,2. However, its biological function in primates remains largely unknown. Here we generate a SIRT6-null cynomolgus monkey (Macaca fascicularis) model using a CRISPR–Cas9-based approach. SIRT6-deficient monkeys die hours after birth and exhibit severe prenatal developmental retardation. SIRT6 loss delays neuronal differentiation by transcriptionally activating the long non-coding RNA H19 (a developmental repressor), and we were able to recapitulate thi...
10 Citations Source Cite
Published on Jan 1, 2018in Cell Research 15.39
Xuepeng Wang5
Estimated H-index: 5
,
Tianda Li9
Estimated H-index: 9
+ 13 AuthorsXinxin Zhang5
Estimated H-index: 5
Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis
7 Citations Source Cite
Published on Jul 1, 2017in National Science Review 9.41
Wei Li41
Estimated H-index: 41
(Chinese Academy of Sciences),
Qi Zhou51
Estimated H-index: 51
(Chinese Academy of Sciences)
1 Citations Source Cite
Published on Apr 2, 2016in Autophagy 11.10
Fuping Wen1
Estimated H-index: 1
(Chinese Academy of Sciences),
Yueshuai Guo10
Estimated H-index: 10
(Nanjing Medical University)
+ 11 AuthorsTao Zhou12
Estimated H-index: 12
(Nanjing Medical University)
ABSTRACTMeiosis is a special type of cellular renovation that involves 2 successive cell divisions and a single round of DNA replication. Two major degradation systems, the autophagy-lysosome and the ubiquitin-proteasome, are involved in meiosis, but their roles have yet to be elucidated. Here we show that autophagy mainly affects the initiation of meiosis but not the nuclear division. Autophagy works not only by serving as a dynamic recycling system but also by eliminating some negative meiotic...
9 Citations Source Cite
Published on Jan 1, 2016in Cell 31.40
Xin Li9
Estimated H-index: 9
(Chinese Academy of Sciences),
Xiaolong Cui3
Estimated H-index: 3
(Chinese Academy of Sciences)
+ 16 AuthorsLing Shuai6
Estimated H-index: 6
(Chinese Academy of Sciences)
Mammalian interspecific hybrids provide unique advantages for mechanistic studies of speciation, gene expression regulation, and X chromosome inactivation (XCI) but are constrained by their limited natural resources. Previous artificially generated mammalian interspecific hybrid cells are usually tetraploids with unstable genomes and limited developmental abilities. Here, we report the generation of mouse-rat allodiploid embryonic stem cells (AdESCs) by fusing haploid ESCs of the two species. Th...
19 Citations Source Cite
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