Julian Grünewald
Harvard University
Publications 3
#1Julian Grünewald (Harvard University)H-Index: 1
#2Ronghao Zhou (Harvard University)H-Index: 1
Last.J. Keith Joung (Harvard University)H-Index: 60
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Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-G alterations1–4. However, we recently demonstrated that they can also induce transcriptome-wide guide-RNA-independent editing of RNA bases5, and created selective curbing of unwanted RNA editing (SECURE)-BE3 variants that have reduced unwanted RNA-editing activity5. Here we describe structure-guided engineering of SECURE-ABE variants with reduced off-target RNA-editing activity and comparable on-target DNA...
#1Julian Grünewald (Harvard University)H-Index: 1
#2Ruobo Zhou (Harvard University)H-Index: 18
Last.J. K. Joung (Harvard University)
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Abstract CRISPR-guided DNA base editors enable the efficient installation of targeted single-nucleotide changes. Cytosine or adenine base editors ( CBE s or ABE s), which are fusions of cytidine or adenosine deaminases to CRISPR-Cas nickases, can efficiently induce DNA C-to-T or A-to-G alterations in DNA, respectively 1-4 . We recently demonstrated that both the widely used CBE BE3 (harboring a rat APOBEC1 cytidine deaminase) and the optimized ABEmax editor can induce tens of thousands of guide ...
#1Julian GrünewaldH-Index: 1
#2Julian Grünewald (Harvard University)
Last.J. Keith JoungH-Index: 60
view all 7 authors...
CRISPR–Cas base-editor technology enables targeted nucleotide alterations, and is being increasingly used for research and potential therapeutic applications1,2. The most widely used cytosine base editors (CBEs) induce deamination of DNA cytosines using the rat APOBEC1 enzyme, which is targeted by a linked Cas protein–guide RNA complex3,4. Previous studies of the specificity of CBEs have identified off-target DNA edits in mammalian cells5,6. Here we show that a CBE with rat APOBEC1 can cause ext...