Tingting Gan
Peking University
Publications 2
#1Yongfeng Yang (PKU: Peking University)H-Index: 2
#2Chuanzhen Yang (PKU: Peking University)H-Index: 2
Last.Xiaofeng Zheng (PKU: Peking University)H-Index: 19
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The DNA damage response (DDR) is essential for maintaining genome integrity. Mounting evidence reveals that protein modifications play vital roles in the DDR. Here, we show that USP38 is involved in the DDR by regulating the activity of HDAC1. In response to DNA damage, USP38 interacted with HDAC1 and specifically removed the K63-linked ubiquitin chain promoting the deacetylase activity of HDAC1. As a result, HDAC1 was able to deacetylate H3K56. USP38 deletion resulted in persistent focal accumu...
#1Jianhang Yin (PKU: Peking University)H-Index: 1
#2Mengzhu Liu (PKU: Peking University)H-Index: 1
Last.Jiazhi Hu (PKU: Peking University)H-Index: 12
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Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq), to comprehensively assess both editing ability and specificity of engineered nucleases. We showed CRISPR/Cas9-generated breaks could lead to chromosomal translocations and large deletions by PEM-seq. ...
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