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Grégoire Cullot
French Institute of Health and Medical Research
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#1Grégoire Cullot (French Institute of Health and Medical Research)H-Index: 1
#2Julian Boutin (French Institute of Health and Medical Research)H-Index: 1
Last.Aurélie BedelH-Index: 5
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CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital erythropoietic porphyria. We demonstrate that homology-directed repair is rare compared with NHEJ pathway leading to on-target indels and causing unwanted dysfunctional protein. Moreover, we describe unexpected chromosomal truncations resulting from only one Cas9 nuclease-induced DSB in cell lines and primary cells by a p53-de...
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