Jianping Yu
Sun Yat-sen University
Embryonic stem cellStem cellBiologyCell biologyCRISPR
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Publications 3
#1Bohong ChenH-Index: 2
Last. Zhou SongyangH-Index: 46
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In mammalian cells, long noncoding RNAs (lncRNAs) form complexes with proteins to execute various biological functions such as gene transcription, RNA processing and other signaling activities. However, methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interacting proteins are limited. Here, we report the development of CERTIS (CRISPR-mediated Endogenous lncRNA Tracking and Immunoprecipitation System) to visualize and isolate endogenous lncRNA, by precisely insertin...
#1Jianping YuH-Index: 1
#2Weisi Lu (SYSU: Sun Yat-sen University)H-Index: 7
Last. Junjiu HuangH-Index: 22
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: The scaffold protein Symplekin (Sympk) is involved in cytoplasmic RNA polyadenylation, transcriptional modulation, and the regulation of epithelial differentiation and proliferation via tight junctions. It is highly expressed in embryonic stem cells (ESCs), in which its role remains unknown. In this study, we found Sympk overexpression in mouse ESCs significantly increased colony formation, and Sympk deletion via CRISPR/Cas9 decreased colony formation. Sympk promoted ESC growth and its overexp...
#1Weisi Lu (SYSU: Sun Yat-sen University)H-Index: 7
#2Jianping Yu (SYSU: Sun Yat-sen University)H-Index: 1
Last. Junjiu Huang (SYSU: Sun Yat-sen University)H-Index: 22
view all 8 authors...
Background Small nucleolar RNA host gene 3 (Snhg3) is a long non-coding RNA (lncRNA) that was shown to participate in the tumorigenesis of certain cancers. However, little is known about its role in embryonic stem cells (ESCs).
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