Rosanna Mattossovich
National Research Council
Publications 3
#1Giada Lo Gullo (Sapienza University of Rome)
#2Rosanna Mattossovich (National Research Council)H-Index: 1
Last.Dario Benelli (Sapienza University of Rome)H-Index: 11
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A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for translation of pretranscribed mRNAs, and now adapted to perform coupled transcription and translation. The essential element in this expression system is a strong promoter derived from the S. solfataricus 16S/23S rRNA-encoding gene, from which specific mR...
#1Sonia Del Prete (National Research Council)H-Index: 22
#2Rosa Merlo (National Research Council)H-Index: 1
Last.Giuseppe Perugino (National Research Council)H-Index: 16
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AbstractCarbonic anhydrases (CAs, EC are a superfamily of ubiquitous metalloenzymes present in all living organisms on the planet. They are classified into seven genetically distinct families and catalyse the hydration reaction of carbon dioxide to bicarbonate and protons, as well as the opposite reaction. CAs were proposed to be used for biotechnological applications, such as the post-combustion carbon capture processes. In this context, there is a great interest in searching CAs with ...
#1Rosa Merlo (National Research Council)H-Index: 1
#2Sonia Del Prete (National Research Council)H-Index: 22
Last.Giuseppe Perugino (National Research Council)H-Index: 16
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AbstractThe use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of proteins on the outer membrane of Gram-negative bacteria. These strategies have the clear advantage of unifying in a one-step the production, the purification and the in vivo immobilisation of proteins/biocatalysts onto a specific biological support. Here, we introduce the novel Anchoring-and-Self-Labelling...