Rosanna Mattossovich
National Research Council
Protein tagEnzymeCarbonic anhydraseThermostabilityBiochemistry
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Publications 5
The genome of living cells is continuously exposed to endogenous and exogenous attacks, and this is particularly amplified at high temperatures. Alkylating agents cause DNA damage, leading to mutations and cell death; for this reason, they also play a central role in chemotherapy treatments. A class of enzymes known as AGTs (alkylguanine-DNA-alkyltransferases) protects the DNA from mutations caused by alkylating agents, in particular in the recognition and repair of alkylated guanines in O6-posi...
#2Rosa MerloH-Index: 1
Last. Giuseppe PeruginoH-Index: 18
view all 8 authors...
The specific labelling of proteins in recent years has made use of self-labelling proteins, such as the SNAP-tag® and the Halotag®. These enzymes, by their nature or suitably engineered, have the ability to specifically react with their respective substrates, but covalently retaining a part of them in the catalytic site upon reaction. This led to the synthesis of substrates conjugated with, e.g., fluorophores (proposing them as alternatives to fluorescent proteins), but also with others chemical...
#1Giada Lo Gullo (Sapienza University of Rome)H-Index: 1
#2Rosanna Mattossovich (National Research Council)H-Index: 2
Last. Dario Benelli (Sapienza University of Rome)H-Index: 12
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A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for translation of pretranscribed mRNAs, and now adapted to perform coupled transcription and translation. The essential element in this expression system is a strong promoter derived from the S. solfataricus 16S/23S rRNA-encoding gene, from which specific mR...
2 CitationsSource
#1Sonia Del Prete (National Research Council)H-Index: 23
#2Rosa Merlo (National Research Council)H-Index: 1
Last. Clemente Capasso (National Research Council)H-Index: 40
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AbstractCarbonic anhydrases (CAs, EC are a superfamily of ubiquitous metalloenzymes present in all living organisms on the planet. They are classified into seven genetically distinct families and catalyse the hydration reaction of carbon dioxide to bicarbonate and protons, as well as the opposite reaction. CAs were proposed to be used for biotechnological applications, such as the post-combustion carbon capture processes. In this context, there is a great interest in searching CAs with ...
1 CitationsSource
#1Rosa Merlo (National Research Council)H-Index: 1
#2Sonia Del Prete (National Research Council)H-Index: 23
Last. Giuseppe Perugino (National Research Council)H-Index: 18
view all 8 authors...
AbstractThe use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of prote...
2 CitationsSource