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Yunxiu Zhang
Dalian Institute of Chemical Physics
2Publications
Publications 2
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#1Yanbin Feng (DICP: Dalian Institute of Chemical Physics)H-Index: 4
#2Yunxiu Zhang (DICP: Dalian Institute of Chemical Physics)
Last.Song Xue (DICP: Dalian Institute of Chemical Physics)H-Index: 18
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Triacylglycerols are considered one of the most promising feedstocks for biofuels. Phospholipid:diacylglycerol acyltransferase (PDAT), responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, has attracted much attention by catalyzing membrane lipid transformation. However, due to lack of biochemical and enzymatic studies, PDAT has not carried forward in biocatalyst application. Here, the PDAT from Saccharomyces cerevisiae was expressed in Pichia pastoris. ...
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#1Yunxiu Zhang (DICP: Dalian Institute of Chemical Physics)
#2Yanbin Feng (DICP: Dalian Institute of Chemical Physics)H-Index: 4
Last.Song Xue (DICP: Dalian Institute of Chemical Physics)H-Index: 18
view all 6 authors...
Glycerol-3-phosphate acyltransferase (GPAT) is considered as the rate-limiting enzyme of glycerolipid synthesis pathway and the core element in lysophosphatidic acid (LPA) synthesis. For understanding its catalytic mechanism, the structural biology study is expected, but is always hindered by obtaining crystals for X-ray diffraction analysis. In this study, a progressive strategy to optimize the crystal of microalgae plastidial GPAT was presented. After the expression and purification of GPAT, t...
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