Michael G. Sargent
Medical Research Council
Publications 4
#1Richard MorganH-Index: 25
#2Michael G. Sargent (Medical Research Council)H-Index: 3
Expression of the RNA-helicase translation initiation factor, eIF4AII, in animal cap explants of Xenopus specifically upregulates genes expressed early in the neural plate border such as Xsna, Xslu, Pax-3 and XANF and also the cement gland marker XCG-1. eIF4AII is expressed specifically in the prospective neurectoderm from stage 11.5 and appears to have a significant role in mediating early patterning of the neurectoderm. It is induced by all known neural inducing regimes including secreted fact...
85 Citations
#1Roberto MayorH-Index: 50
#2Richard MorganH-Index: 25
Last.Michael G. SargentH-Index: 3
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The earliest sign of the prospective neural crest of Xenopus is the expression of the ectodermal component of Xsna (the Xenopus homologue of snail) in a low arc on the dorsal aspect of stage 11 embryos, which subsequently assumes the horseshoe shape characteristic of the neural folds as the convergence-extension movements shape the neural plate. A related zinc-finger gene called Slug (Xslu) is expressed specifically in this tissue (i.e. the prospective crest) when the convergence extension movem...
411 Citations
#1Roberto MayorH-Index: 50
#2Linda J. EssexH-Index: 2
Last.Michael G. SargentH-Index: 3
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Xsna, the Xenopus homologue of Drosophila snail, is expressed in both mesoderm and ectoderm. Expression occurs in all mesoderm initially but is down regulated in a tissue-specific fashion at the end of gastrulation in a way that reveals the subdivision of the mesoderm before its derivatives are overtly differentiated. Xsna is also expressed in the ectoderm of the prospective neural fold from stage 11, in a distinct band of cells surrounding the prospective neural plate, which we designate the ne...
50 Citations
#1Michael G. SargentH-Index: 3
#2Susan DaviesH-Index: 1
Last.Michael F. BennettH-Index: 2
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Summary: In several strains of Bacillus subtilis extensive breakdown of chromosomal DNA may be potentiated by osmotic lysis of protoplasts. At its most severe, in strains originating from Farmer & Rothman's thymine auxotroph, the rate of DNA breakdown was greater than 50% per hour at 40 °C. The rate of DNA breakdown in most other strains tested was approximately 5% per hour except for SP.-strains, in which the rate of DNA breakdown was only 0.3%. DNA degradation was attributed to relaxation of c...
3 CitationsSource