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Xiao Hu
Yale University
Somatic cellStem cellReprogrammingBiologyCell biology
11Publications
2H-index
336Citations
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Publications 15
Newest
#1Amaleah Hartman (Yale University)H-Index: 1
#2S. Maxwell Scalf (Yale University)H-Index: 1
Last. Shangqin Guo (Yale University)H-Index: 12
view all 8 authors...
Summary Yes-associated protein (YAP) is known to promote the stemness of multiple stem cell types, including pluripotent stem cells, while also antagonizing pluripotency during early embryogenesis. How YAP accomplishes these distinct functions remains unclear. Here, we report that, depending on the specific cells in which it is expressed, YAP could exhibit opposing effects on pluripotency induction from mouse somatic cells. Specifically, YAP inhibits pluripotency induction cell-autonomously but ...
1 CitationsSource
#1Xiao Hu (Yale University)H-Index: 2
#2Qiao Wu (Yale University)
Last. Shangqin Guo (Yale University)H-Index: 12
view all 7 authors...
There is wide variability in the propensity of somatic cells to reprogram into pluripotency in response to the Yamanaka factors. How to segregate these variability to enrich for cells of specific traits that reprogram efficiently remains challenging. Here we report that the variability in reprogramming propensity is associated with the activity of the MKL1/SRF transcription factor and concurs with small cell size as well as rapid cell cycle. Reprogramming progressive cells can be prospectively i...
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#1Xinyue Chen (Yale University)H-Index: 1
#2Daniel Burkhardt (Yale University)H-Index: 5
Last. Shangqin Guo (Yale University)H-Index: 12
view all 10 authors...
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#1Xinyue Chen (Yale University)H-Index: 1
#2Daniel Burkhardt (Yale University)H-Index: 5
Last. Shangqin Guo (Yale University)H-Index: 12
view all 10 authors...
Cancer is a hyper-proliferative disease. Whether the proliferative state originates from the cell-of-origin or emerges later remains difficult to resolve. By tracking de novo transformation from normal hematopoietic progenitors expressing an acute myeloid leukemia (AML) oncogene MLL-AF9, we reveal that the cell cycle rate heterogeneity among granulocyte–macrophage progenitors (GMPs) determines their probability of transformation. A fast cell cycle intrinsic to these progenitors provide permissiv...
Source
#1Xiao Hu (Yale University)H-Index: 2
#2Shangqin Guo (Yale University)H-Index: 12
Why most cells remain refractory to transcription factor (TF)-induced fate conversion remains largely mysterious, with the answers holding important instructions on how to effectively direct cell identities. In this issue of Cell Stem Cell, Babos et al. (2019) show that conflicts caused by simultaneous high transcription and high replication rates are to blame.
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#1Xiao Hu (Yale University)H-Index: 2
#2Anna E. Eastman (Yale University)H-Index: 1
Last. Shangqin Guo (Yale University)H-Index: 12
view all 3 authors...
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#1Xiao Hu (Yale University)H-Index: 2
#2Zongzhi Liu (Yale University)H-Index: 11
Last. Shangqin Guo (Yale University)H-Index: 12
view all 20 authors...
Actin cytoskeleton is well-known for providing structural/mechanical support, but whether and how it regulates chromatin and cell fate reprogramming is far less clear. Here, we report that MKL1, the key transcriptional co-activator of many actin cytoskeletal genes, regulates genomic accessibility and cell fate reprogramming. The MKL1-actin pathway weakens during somatic cell reprogramming by pluripotency transcription factors. Cells that reprogram efficiently display low endogenous MKL1 and inhi...
1 CitationsSource
#1Anna E. Eastman (Yale University)H-Index: 1
#2Xinyue Chen (Yale University)H-Index: 1
Last. Shangqin Guo (Yale University)H-Index: 12
view all 9 authors...
Periodicity is a fundamental property of biological oscillators such as the mitotic cell cycle. In this context, periodicity refers to the time interval between the same phases of two consecutive cell cycles. The length of this interval, or the cell cycle speed, varies widely depending on cell type and the pathophysiological conditions. The relevance of cell cycle speed in various biological contexts has not been well-studied, partially due to the lack of experimental approaches that capture thi...
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#1Xinyue Chen (Yale University)H-Index: 1
#2Amaleah Hartman (Yale University)H-Index: 1
Last. Shangqin Guo (Yale University)H-Index: 12
view all 7 authors...
Cancer is a hyper-proliferative clonal disease. Whether the proliferative state originates from the cell-of-origin or emerges later remains elusive. By tracking de novo transformation from normal hematopoietic progenitors expressing an acute myeloid leukemia (AML) oncogene MLL-AF9, we reveal that the cell cycle rate heterogeneity among granulocyte-macrophage progenitors (GMPs) determines their probability of transformation. An intrinsic fast cell cycle kinetics at the time of oncogene expression...
1 CitationsSource
#1Amaleah Hartman (Yale University)H-Index: 1
#2Xiao Hu (Yale University)H-Index: 2
Last. Shangqin Guo (Yale University)H-Index: 12
view all 6 authors...
While Yes-associated protein (YAP) antagonizes pluripotency during early embryogenesis, it has also been shown to promote stemness of multiple stem cell types, including pluripotent stem cells. Whether cellular context underlies these distinct functions of YAP in pluripotency remains unclear. Here, we establish that depending on the specific cells in which it is expressed, YAP exhibits opposing effects on pluripotency induction from somatic cells. Specifically, YAP inhibits pluripotency inductio...
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